Quantification of proliferative and suppressive responses of human T lymphocytes following ConA stimulation

The mitogenic response of human T lymphocytes to graded doses of concanavalin A (ConA) has been measured by means of an MTT tetrazolium dye metabolic assay. Three groups of healthy subjects, representing children, younger adults and elderly persons, were investigated. It was shown that a typical bel...

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Veröffentlicht in:	Journal of immunological methods 1990-02, Vol.126 (2), p.263-271
Hauptverfasser:	Nikš, M., Otto, M., Bušová, B., Štefanović, J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging - immunology Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte - immunology Biological and medical sciences CD8 Antigens Cell Division - drug effects Cell Survival - drug effects Colorimetry - methods Concanavalin A - pharmacology Concanavalin A dose-response Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Kinetics Lymphocyte Activation - drug effects Lymphocyte Activation - immunology Male Mathematical model Models, Biological Molecular immunology Proliferation Suppression T lymphocyte T-Lymphocytes - drug effects T-Lymphocytes - immunology T-Lymphocytes, Regulatory - drug effects T-Lymphocytes, Regulatory - immunology Techniques Tetrazolium Salts Thiazoles
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