MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma
•Levels of miR-320a are frequently lower in NPC cell lines and tissues.•Overexpression of miR-320a suppresses NPC cell proliferation in vitro and in vivo.•Overexpression of miR-320a reduces migration and invasion of NPC cells.•miR-320a directly and functionally targets BMI-1.•BMI-1 is up-regulated i...
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| Veröffentlicht in: | FEBS letters 2014-10, Vol.588 (20), p.3732-3738 |
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| creator | Qi, Xiaoming Li, Jianqiang Zhou, Changbo Lv, Chunlei Tian, Min |
| description | •Levels of miR-320a are frequently lower in NPC cell lines and tissues.•Overexpression of miR-320a suppresses NPC cell proliferation in vitro and in vivo.•Overexpression of miR-320a reduces migration and invasion of NPC cells.•miR-320a directly and functionally targets BMI-1.•BMI-1 is up-regulated in NPC specimens and inversely correlates with miR-320a levels.
In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC. |
| doi_str_mv | 10.1016/j.febslet.2014.08.021 |
| format | Article |
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In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.08.021</identifier><identifier>PMID: 25171860</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>3' Untranslated Regions ; Animals ; BMI-1 ; Carcinoma - genetics ; Carcinoma - metabolism ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Humans ; Invasion ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-320a ; Nasopharyngeal carcinoma ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Neoplasm Invasiveness ; Neoplasms, Experimental - metabolism ; Neoplasms, Experimental - pathology ; Polycomb Repressive Complex 1 - genetics ; Polycomb Repressive Complex 1 - metabolism ; Proliferation ; Up-Regulation</subject><ispartof>FEBS letters, 2014-10, Vol.588 (20), p.3732-3738</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 588 (2014) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5001-c793b425c8567dc32fcc52c3e821d4015443b3d9e3ceafadd435d038d2698d433</citedby><cites>FETCH-LOGICAL-c5001-c793b425c8567dc32fcc52c3e821d4015443b3d9e3ceafadd435d038d2698d433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2014.08.021$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2014.08.021$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,1412,1428,3537,27905,27906,45555,45556,45976,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25171860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qi, Xiaoming</creatorcontrib><creatorcontrib>Li, Jianqiang</creatorcontrib><creatorcontrib>Zhou, Changbo</creatorcontrib><creatorcontrib>Lv, Chunlei</creatorcontrib><creatorcontrib>Tian, Min</creatorcontrib><title>MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Levels of miR-320a are frequently lower in NPC cell lines and tissues.•Overexpression of miR-320a suppresses NPC cell proliferation in vitro and in vivo.•Overexpression of miR-320a reduces migration and invasion of NPC cells.•miR-320a directly and functionally targets BMI-1.•BMI-1 is up-regulated in NPC specimens and inversely correlates with miR-320a levels.
In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC.</description><subject>3' Untranslated Regions</subject><subject>Animals</subject><subject>BMI-1</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Invasion</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-320a</subject><subject>Nasopharyngeal carcinoma</subject><subject>Nasopharyngeal Neoplasms - genetics</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Neoplasm Invasiveness</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Polycomb Repressive Complex 1 - genetics</subject><subject>Polycomb Repressive Complex 1 - metabolism</subject><subject>Proliferation</subject><subject>Up-Regulation</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkclu2zAQhomiQeIsj9CCxx4ilYso06fCCZwFcBIgy5mgyJFDQ6JcUnbgtw8Fub22J3Iw_8z88w1C3yjJKaHlz3VeQxUb6HNGaJETmRNGv6AJlVOe8aKUX9GEpEwmpjN-gk5jXJMUSzo7RidM0CmVJZmgzYMzoXt-nGecEY2df3eV6yM20DR4E7rG1RB07zp_iVu3Gr9Ye5ukOx2HoNrjXocV9M6v8NXDfUZTDnsdu827Dnu_At1go4Nxvmv1OTqqdRPh4vCeobebxev1XbZ8ur2_ni8zI5LNzCTXVcGEkaKcWsNZbYxghoNk1BaEiqLgFbcz4AZ0ra0tuLCES8vKmUwBP0M_xr5pid9biL1qXRy20h66bVRUlCVNJJlMUjFKE4kYA9RqE1ybrCtK1ABbrdUBthpgKyJVgp3qvh9GbKsW7N-qP3ST4G4UfLgG9v_XVd0srtjLcLnhcLQgpGR8mPVrbAWJ2c5BUNE48AasC2B6ZTv3D7efX1SoSA</recordid><startdate>20141016</startdate><enddate>20141016</enddate><creator>Qi, Xiaoming</creator><creator>Li, Jianqiang</creator><creator>Zhou, Changbo</creator><creator>Lv, Chunlei</creator><creator>Tian, Min</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141016</creationdate><title>MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma</title><author>Qi, Xiaoming ; Li, Jianqiang ; Zhou, Changbo ; Lv, Chunlei ; Tian, Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5001-c793b425c8567dc32fcc52c3e821d4015443b3d9e3ceafadd435d038d2698d433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3' Untranslated Regions</topic><topic>Animals</topic><topic>BMI-1</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Invasion</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-320a</topic><topic>Nasopharyngeal carcinoma</topic><topic>Nasopharyngeal Neoplasms - genetics</topic><topic>Nasopharyngeal Neoplasms - metabolism</topic><topic>Neoplasm Invasiveness</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Polycomb Repressive Complex 1 - genetics</topic><topic>Polycomb Repressive Complex 1 - metabolism</topic><topic>Proliferation</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qi, Xiaoming</creatorcontrib><creatorcontrib>Li, Jianqiang</creatorcontrib><creatorcontrib>Zhou, Changbo</creatorcontrib><creatorcontrib>Lv, Chunlei</creatorcontrib><creatorcontrib>Tian, Min</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qi, Xiaoming</au><au>Li, Jianqiang</au><au>Zhou, Changbo</au><au>Lv, Chunlei</au><au>Tian, Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2014-10-16</date><risdate>2014</risdate><volume>588</volume><issue>20</issue><spage>3732</spage><epage>3738</epage><pages>3732-3738</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Levels of miR-320a are frequently lower in NPC cell lines and tissues.•Overexpression of miR-320a suppresses NPC cell proliferation in vitro and in vivo.•Overexpression of miR-320a reduces migration and invasion of NPC cells.•miR-320a directly and functionally targets BMI-1.•BMI-1 is up-regulated in NPC specimens and inversely correlates with miR-320a levels.
In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft mouse model. A luciferase reporter assay revealed that miR-320a could directly bind to the 3′ UTR of BMI-1. Overexpression of BMI-1 rescued miR-320a-mediated biological function. BMI-1 expression was found to be up-regulated and inversely correlated with miR-320a expression in NPC. Collectively, our data indicate that miR-320a plays a tumor suppressor role in the development and progression of NPC and may be a novel therapeutic target against NPC.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25171860</pmid><doi>10.1016/j.febslet.2014.08.021</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3' Untranslated Regions Animals BMI-1 Carcinoma - genetics Carcinoma - metabolism Cell Line, Tumor Cell Movement Cell Proliferation Gene Expression Regulation, Neoplastic Humans Invasion Mice Mice, Inbred BALB C Mice, Nude MicroRNAs - genetics MicroRNAs - metabolism miR-320a Nasopharyngeal carcinoma Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Neoplasm Invasiveness Neoplasms, Experimental - metabolism Neoplasms, Experimental - pathology Polycomb Repressive Complex 1 - genetics Polycomb Repressive Complex 1 - metabolism Proliferation Up-Regulation |
| title | MicroRNA-320a inhibits cell proliferation, migration and invasion by targeting BMI-1 in nasopharyngeal carcinoma |
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