P sub(2)-purinoceptor-activated membrane currents and inositol tetrakisphosphate formation are blocked by suramin
The effect of suramin on the ATP-induced response in vas deferens DDT sub(1) MF-2 smooth muscle cells was studied. Stimulation of P sub(2)-purinoceptors by ATP caused a change in membrane currents, measured by using the whole-cell patch-clamp configuration, and enhanced the formation of inositol pho...
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| Veröffentlicht in: | European journal of pharmacology 1990-01, Vol.181 (1-2), p.127-131 |
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| container_end_page | 131 |
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| container_issue | 1-2 |
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| container_title | European journal of pharmacology |
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| creator | Hoiting, B Molleman, A Nelemans, A Den Hertog, A |
| description | The effect of suramin on the ATP-induced response in vas deferens DDT sub(1) MF-2 smooth muscle cells was studied. Stimulation of P sub(2)-purinoceptors by ATP caused a change in membrane currents, measured by using the whole-cell patch-clamp configuration, and enhanced the formation of inositol phosphates, as analysed by high performance liquid chromatography. The ATP-induced membrane current consisted of a triphasic response, carried by a fast inward current, followed by a transient outward current and a sustained inward current. Inositol tetrakisphosphate (InsP sub(4)) formation increased in the presence of ATP. The results indicate that suramin inhibits ATP-activated cellular processes in DDT sub(1) MF-2 vas deferens cells, most likely by acting on P sub(2)-purinoceptors. |
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Stimulation of P sub(2)-purinoceptors by ATP caused a change in membrane currents, measured by using the whole-cell patch-clamp configuration, and enhanced the formation of inositol phosphates, as analysed by high performance liquid chromatography. The ATP-induced membrane current consisted of a triphasic response, carried by a fast inward current, followed by a transient outward current and a sustained inward current. Inositol tetrakisphosphate (InsP sub(4)) formation increased in the presence of ATP. The results indicate that suramin inhibits ATP-activated cellular processes in DDT sub(1) MF-2 vas deferens cells, most likely by acting on P sub(2)-purinoceptors.</description><identifier>ISSN: 0014-2999</identifier><language>eng</language><subject>ATP ; inositol tetrakisphosphate ; smooth muscle ; suramin ; vas deferens</subject><ispartof>European journal of pharmacology, 1990-01, Vol.181 (1-2), p.127-131</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Hoiting, B</creatorcontrib><creatorcontrib>Molleman, A</creatorcontrib><creatorcontrib>Nelemans, A</creatorcontrib><creatorcontrib>Den Hertog, A</creatorcontrib><title>P sub(2)-purinoceptor-activated membrane currents and inositol tetrakisphosphate formation are blocked by suramin</title><title>European journal of pharmacology</title><description>The effect of suramin on the ATP-induced response in vas deferens DDT sub(1) MF-2 smooth muscle cells was studied. Stimulation of P sub(2)-purinoceptors by ATP caused a change in membrane currents, measured by using the whole-cell patch-clamp configuration, and enhanced the formation of inositol phosphates, as analysed by high performance liquid chromatography. The ATP-induced membrane current consisted of a triphasic response, carried by a fast inward current, followed by a transient outward current and a sustained inward current. Inositol tetrakisphosphate (InsP sub(4)) formation increased in the presence of ATP. The results indicate that suramin inhibits ATP-activated cellular processes in DDT sub(1) MF-2 vas deferens cells, most likely by acting on P sub(2)-purinoceptors.</description><subject>ATP</subject><subject>inositol tetrakisphosphate</subject><subject>smooth muscle</subject><subject>suramin</subject><subject>vas deferens</subject><issn>0014-2999</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqNjbFOBDEMRFOAxMHxD64QFCtlFxaUGoEoKehP3pxPhEviPdtB4u9JwQdQjKZ5M-_MbbwfH4YphHDhLlW_vPdzmOaNO72DtuV2uhvWJqlypNVYBoyWvtFoD4XKIlgJYhOhagpY99BJTcYZjEzwmHT95J6-gANLQUtcAYVgyRyP_Wb56R7BkurWnR8wK13_9ZW7eX35eH4bVuFTI7VdSRop5y7lprtxfpz90xju_w3-AitgTvo</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Hoiting, B</creator><creator>Molleman, A</creator><creator>Nelemans, A</creator><creator>Den Hertog, A</creator><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19900101</creationdate><title>P sub(2)-purinoceptor-activated membrane currents and inositol tetrakisphosphate formation are blocked by suramin</title><author>Hoiting, B ; Molleman, A ; Nelemans, A ; Den Hertog, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_156507193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ATP</topic><topic>inositol tetrakisphosphate</topic><topic>smooth muscle</topic><topic>suramin</topic><topic>vas deferens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoiting, B</creatorcontrib><creatorcontrib>Molleman, A</creatorcontrib><creatorcontrib>Nelemans, A</creatorcontrib><creatorcontrib>Den Hertog, A</creatorcontrib><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoiting, B</au><au>Molleman, A</au><au>Nelemans, A</au><au>Den Hertog, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>P sub(2)-purinoceptor-activated membrane currents and inositol tetrakisphosphate formation are blocked by suramin</atitle><jtitle>European journal of pharmacology</jtitle><date>1990-01-01</date><risdate>1990</risdate><volume>181</volume><issue>1-2</issue><spage>127</spage><epage>131</epage><pages>127-131</pages><issn>0014-2999</issn><abstract>The effect of suramin on the ATP-induced response in vas deferens DDT sub(1) MF-2 smooth muscle cells was studied. Stimulation of P sub(2)-purinoceptors by ATP caused a change in membrane currents, measured by using the whole-cell patch-clamp configuration, and enhanced the formation of inositol phosphates, as analysed by high performance liquid chromatography. The ATP-induced membrane current consisted of a triphasic response, carried by a fast inward current, followed by a transient outward current and a sustained inward current. Inositol tetrakisphosphate (InsP sub(4)) formation increased in the presence of ATP. The results indicate that suramin inhibits ATP-activated cellular processes in DDT sub(1) MF-2 vas deferens cells, most likely by acting on P sub(2)-purinoceptors.</abstract></addata></record> |
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| ispartof | European journal of pharmacology, 1990-01, Vol.181 (1-2), p.127-131 |
| issn | 0014-2999 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15650719 |
| source | Elsevier ScienceDirect Journals Complete |
| subjects | ATP inositol tetrakisphosphate smooth muscle suramin vas deferens |
| title | P sub(2)-purinoceptor-activated membrane currents and inositol tetrakisphosphate formation are blocked by suramin |
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