Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus
Persistent infection of the chestnut blight fungus Cryphonectria parasitica with the prototypic hypovirus CHV1-713 results in attenuation of fungal virulence (hypovirulence) and reduced accumulation of the GTP-binding (G) protein alpha subunit CPG-1. Transgenic cosuppression of CPG-1 accumulation in...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-07, Vol.93 (15), p.7996-8000 |
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| creator | Chen, B. (University of Maryland, College Park, MD.) Gao, S Choi, G.H Nuss, D.L |
| description | Persistent infection of the chestnut blight fungus Cryphonectria parasitica with the prototypic hypovirus CHV1-713 results in attenuation of fungal virulence (hypovirulence) and reduced accumulation of the GTP-binding (G) protein alpha subunit CPG-1. Transgenic cosuppression of CPG-1 accumulation in the absence of virus infection also confers hypovirulence. We now report the use of mRNA differential display to examine the extent to which virus infection alters fungal gene transcript accumulation and to assess the degree to which modification of CPG-1 signal transduction contributes to this alteration. More than 400 PCR products were identified that either increased (296 products) or decreased (127 products) in abundance as a result of virus infection. Significantly, 65% of these products exhibited similar changes as a result of CPG-1 cosuppression in the absence of virus infection. We also report that both virus infection and CPG-1 cosuppression elevate cAMP levels 3- to 5-fold. Additionally, it was possible to mimic the effect of virus infection and CPG-1 cosuppression on transcript accumulation for representative fungal genes by drug-induced elevation of cAMP levels. These results strengthen and extend previous indications that hypovirus infection causes a significant and persistent alteration of fungal gene expression/transcript accumulation. They further show that this alteration is primarily mediated through modification of the CPG-1 signaling pathway and suggest that, similar to mammalian Gi; alpha subunits, CPG-1 functions as negative modulator of adenylyl cyclase. Finally, these results suggest a role for G-protein-regulated cAMP accumulation in hypovirus-mediated alteration of fungal gene expression |
| doi_str_mv | 10.1073/pnas.93.15.7996 |
| format | Article |
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(University of Maryland, College Park, MD.) ; Gao, S ; Choi, G.H ; Nuss, D.L</creator><creatorcontrib>Chen, B. (University of Maryland, College Park, MD.) ; Gao, S ; Choi, G.H ; Nuss, D.L</creatorcontrib><description>Persistent infection of the chestnut blight fungus Cryphonectria parasitica with the prototypic hypovirus CHV1-713 results in attenuation of fungal virulence (hypovirulence) and reduced accumulation of the GTP-binding (G) protein alpha subunit CPG-1. Transgenic cosuppression of CPG-1 accumulation in the absence of virus infection also confers hypovirulence. We now report the use of mRNA differential display to examine the extent to which virus infection alters fungal gene transcript accumulation and to assess the degree to which modification of CPG-1 signal transduction contributes to this alteration. More than 400 PCR products were identified that either increased (296 products) or decreased (127 products) in abundance as a result of virus infection. Significantly, 65% of these products exhibited similar changes as a result of CPG-1 cosuppression in the absence of virus infection. We also report that both virus infection and CPG-1 cosuppression elevate cAMP levels 3- to 5-fold. Additionally, it was possible to mimic the effect of virus infection and CPG-1 cosuppression on transcript accumulation for representative fungal genes by drug-induced elevation of cAMP levels. These results strengthen and extend previous indications that hypovirus infection causes a significant and persistent alteration of fungal gene expression/transcript accumulation. They further show that this alteration is primarily mediated through modification of the CPG-1 signaling pathway and suggest that, similar to mammalian Gi; alpha subunits, CPG-1 functions as negative modulator of adenylyl cyclase. Finally, these results suggest a role for G-protein-regulated cAMP accumulation in hypovirus-mediated alteration of fungal gene expression</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.93.15.7996</identifier><identifier>PMID: 8755591</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>1-Methyl-3-isobutylxanthine - pharmacology ; ADENOSINE MONOPHOSPHATE ; ADENOSINMONOFOSFATO ; ARN MENSAJERO ; ARN MESSAGER ; Caffeine - pharmacology ; CRYPHONECTRIA PARASITICA ; Cyclic AMP - metabolism ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fungal genes ; Fungal Proteins - biosynthesis ; Fungi ; Genes, Fungal ; GENETICA ; GENETIQUE ; GTP-Binding Protein alpha Subunits, Gi-Go ; GTP-Binding Proteins - biosynthesis ; Heterotrimeric GTP-Binding Proteins ; hypovirus ; Infections ; Messenger RNA ; Microbiology ; NUCLEOTIDE ; NUCLEOTIDOS ; Parasitism ; PATHOGENESE ; PATOGENESIS ; PATOGENICIDAD ; Polymerase Chain Reaction ; POUVOIR PATHOGENE ; Product category rules ; Product displays ; PROTEINAS AGLUTINANTES ; PROTEINE DE LIAISON ; Proteins ; Ribonucleic acid ; RNA ; RNA Viruses - physiology ; RNA, Fungal - biosynthesis ; RNA, Messenger - biosynthesis ; Signal Transduction ; Theophylline - pharmacology ; TRANSCRIPCION ; TRANSCRIPTION ; Transcription, Genetic ; Virulence ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX ; Virus Replication ; Viruses ; Xylariales - pathogenicity ; Xylariales - physiology ; Xylariales - virology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-07, Vol.93 (15), p.7996-8000</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Jul 23, 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c537t-99c9bc07e3b92972508ecd77d0528de8c6a24c1126a758a11d3c09fae2aaf4fc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/93/15.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40151$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40151$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,725,778,782,801,883,27911,27912,53778,53780,58004,58237</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8755591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, B. (University of Maryland, College Park, MD.)</creatorcontrib><creatorcontrib>Gao, S</creatorcontrib><creatorcontrib>Choi, G.H</creatorcontrib><creatorcontrib>Nuss, D.L</creatorcontrib><title>Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Persistent infection of the chestnut blight fungus Cryphonectria parasitica with the prototypic hypovirus CHV1-713 results in attenuation of fungal virulence (hypovirulence) and reduced accumulation of the GTP-binding (G) protein alpha subunit CPG-1. Transgenic cosuppression of CPG-1 accumulation in the absence of virus infection also confers hypovirulence. We now report the use of mRNA differential display to examine the extent to which virus infection alters fungal gene transcript accumulation and to assess the degree to which modification of CPG-1 signal transduction contributes to this alteration. More than 400 PCR products were identified that either increased (296 products) or decreased (127 products) in abundance as a result of virus infection. Significantly, 65% of these products exhibited similar changes as a result of CPG-1 cosuppression in the absence of virus infection. We also report that both virus infection and CPG-1 cosuppression elevate cAMP levels 3- to 5-fold. Additionally, it was possible to mimic the effect of virus infection and CPG-1 cosuppression on transcript accumulation for representative fungal genes by drug-induced elevation of cAMP levels. These results strengthen and extend previous indications that hypovirus infection causes a significant and persistent alteration of fungal gene expression/transcript accumulation. They further show that this alteration is primarily mediated through modification of the CPG-1 signaling pathway and suggest that, similar to mammalian Gi; alpha subunits, CPG-1 functions as negative modulator of adenylyl cyclase. Finally, these results suggest a role for G-protein-regulated cAMP accumulation in hypovirus-mediated alteration of fungal gene expression</description><subject>1-Methyl-3-isobutylxanthine - pharmacology</subject><subject>ADENOSINE MONOPHOSPHATE</subject><subject>ADENOSINMONOFOSFATO</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Caffeine - pharmacology</subject><subject>CRYPHONECTRIA PARASITICA</subject><subject>Cyclic AMP - metabolism</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fungal genes</subject><subject>Fungal Proteins - biosynthesis</subject><subject>Fungi</subject><subject>Genes, Fungal</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>GTP-Binding Protein alpha Subunits, Gi-Go</subject><subject>GTP-Binding Proteins - biosynthesis</subject><subject>Heterotrimeric GTP-Binding Proteins</subject><subject>hypovirus</subject><subject>Infections</subject><subject>Messenger RNA</subject><subject>Microbiology</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>Parasitism</subject><subject>PATHOGENESE</subject><subject>PATOGENESIS</subject><subject>PATOGENICIDAD</subject><subject>Polymerase Chain Reaction</subject><subject>POUVOIR PATHOGENE</subject><subject>Product category rules</subject><subject>Product displays</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE DE LIAISON</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA Viruses - physiology</subject><subject>RNA, Fungal - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Signal Transduction</subject><subject>Theophylline - pharmacology</subject><subject>TRANSCRIPCION</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><subject>Virulence</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><subject>Virus Replication</subject><subject>Viruses</subject><subject>Xylariales - pathogenicity</subject><subject>Xylariales - physiology</subject><subject>Xylariales - virology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9rFDEYhwdR6lo9C4ISPOhptvkzmSTgpZRahYqC9hyymXems8wmY5JZuh_Db2yGXbfqQU85_J7nTd7wK4rnBC8JFuxsdCYuFVsSvhRK1Q-KBcGKlHWl8MNigTEVpaxo9bh4EuMaY6y4xCfFiRScc0UWxY_LuwQu9ltAZkgQTOq9Q75F7eQ6M6AOHKAUjIs29GNCxtppMw17zLgGwQDbo3RVjsEn6F0ZoJspaJA9__QFZQiGiFY7ZNC2D9MAzkJpUr57yrbr0O1u9HMSnxaPWjNEeHY4T4ub95ffLj6U15-vPl6cX5eWM5FKpaxaWSyArRRVgnIswTZCNJhT2YC0taGVJYTWRnBpCGmYxao1QI1pq9ay0-Ldfu44rTbQWHB5zUGPod-YsNPe9PrPxPW3uvNbzaSsWdbfHPTgv08Qk9700cIwGAd-ilpIKiih-L8g4XXFMaMZfP0XuPZTcPkPNMWECc6wzNDZHrLBxxigPT6YYD03Qs-N0IrluXpuRDZe_r7nkT9UIOdvD_ks_krvB-h2GnIz7lImX_2TzMCLPbCOyYcjUWHCyX3YGq9NF_qob76qmkrFFfsJ5ATf8Q</recordid><startdate>19960723</startdate><enddate>19960723</enddate><creator>Chen, B. (University of Maryland, College Park, MD.)</creator><creator>Gao, S</creator><creator>Choi, G.H</creator><creator>Nuss, D.L</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960723</creationdate><title>Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus</title><author>Chen, B. (University of Maryland, College Park, MD.) ; Gao, S ; Choi, G.H ; Nuss, D.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c537t-99c9bc07e3b92972508ecd77d0528de8c6a24c1126a758a11d3c09fae2aaf4fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>1-Methyl-3-isobutylxanthine - pharmacology</topic><topic>ADENOSINE MONOPHOSPHATE</topic><topic>ADENOSINMONOFOSFATO</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Caffeine - pharmacology</topic><topic>CRYPHONECTRIA PARASITICA</topic><topic>Cyclic AMP - metabolism</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fungal genes</topic><topic>Fungal Proteins - biosynthesis</topic><topic>Fungi</topic><topic>Genes, Fungal</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>GTP-Binding Protein alpha Subunits, Gi-Go</topic><topic>GTP-Binding Proteins - biosynthesis</topic><topic>Heterotrimeric GTP-Binding Proteins</topic><topic>hypovirus</topic><topic>Infections</topic><topic>Messenger RNA</topic><topic>Microbiology</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>Parasitism</topic><topic>PATHOGENESE</topic><topic>PATOGENESIS</topic><topic>PATOGENICIDAD</topic><topic>Polymerase Chain Reaction</topic><topic>POUVOIR PATHOGENE</topic><topic>Product category rules</topic><topic>Product displays</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINE DE LIAISON</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA Viruses - physiology</topic><topic>RNA, Fungal - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Signal Transduction</topic><topic>Theophylline - pharmacology</topic><topic>TRANSCRIPCION</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic</topic><topic>Virulence</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><topic>Virus Replication</topic><topic>Viruses</topic><topic>Xylariales - pathogenicity</topic><topic>Xylariales - physiology</topic><topic>Xylariales - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, B. (University of Maryland, College Park, MD.)</creatorcontrib><creatorcontrib>Gao, S</creatorcontrib><creatorcontrib>Choi, G.H</creatorcontrib><creatorcontrib>Nuss, D.L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, B. (University of Maryland, College Park, MD.)</au><au>Gao, S</au><au>Choi, G.H</au><au>Nuss, D.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1996-07-23</date><risdate>1996</risdate><volume>93</volume><issue>15</issue><spage>7996</spage><epage>8000</epage><pages>7996-8000</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>Persistent infection of the chestnut blight fungus Cryphonectria parasitica with the prototypic hypovirus CHV1-713 results in attenuation of fungal virulence (hypovirulence) and reduced accumulation of the GTP-binding (G) protein alpha subunit CPG-1. Transgenic cosuppression of CPG-1 accumulation in the absence of virus infection also confers hypovirulence. We now report the use of mRNA differential display to examine the extent to which virus infection alters fungal gene transcript accumulation and to assess the degree to which modification of CPG-1 signal transduction contributes to this alteration. More than 400 PCR products were identified that either increased (296 products) or decreased (127 products) in abundance as a result of virus infection. Significantly, 65% of these products exhibited similar changes as a result of CPG-1 cosuppression in the absence of virus infection. We also report that both virus infection and CPG-1 cosuppression elevate cAMP levels 3- to 5-fold. Additionally, it was possible to mimic the effect of virus infection and CPG-1 cosuppression on transcript accumulation for representative fungal genes by drug-induced elevation of cAMP levels. These results strengthen and extend previous indications that hypovirus infection causes a significant and persistent alteration of fungal gene expression/transcript accumulation. They further show that this alteration is primarily mediated through modification of the CPG-1 signaling pathway and suggest that, similar to mammalian Gi; alpha subunits, CPG-1 functions as negative modulator of adenylyl cyclase. Finally, these results suggest a role for G-protein-regulated cAMP accumulation in hypovirus-mediated alteration of fungal gene expression</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8755591</pmid><doi>10.1073/pnas.93.15.7996</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-07, Vol.93 (15), p.7996-8000 |
| issn | 0027-8424 1091-6490 |
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| source | MEDLINE; Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 1-Methyl-3-isobutylxanthine - pharmacology ADENOSINE MONOPHOSPHATE ADENOSINMONOFOSFATO ARN MENSAJERO ARN MESSAGER Caffeine - pharmacology CRYPHONECTRIA PARASITICA Cyclic AMP - metabolism EXPRESION GENICA EXPRESSION DES GENES Fungal genes Fungal Proteins - biosynthesis Fungi Genes, Fungal GENETICA GENETIQUE GTP-Binding Protein alpha Subunits, Gi-Go GTP-Binding Proteins - biosynthesis Heterotrimeric GTP-Binding Proteins hypovirus Infections Messenger RNA Microbiology NUCLEOTIDE NUCLEOTIDOS Parasitism PATHOGENESE PATOGENESIS PATOGENICIDAD Polymerase Chain Reaction POUVOIR PATHOGENE Product category rules Product displays PROTEINAS AGLUTINANTES PROTEINE DE LIAISON Proteins Ribonucleic acid RNA RNA Viruses - physiology RNA, Fungal - biosynthesis RNA, Messenger - biosynthesis Signal Transduction Theophylline - pharmacology TRANSCRIPCION TRANSCRIPTION Transcription, Genetic Virulence VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus Replication Viruses Xylariales - pathogenicity Xylariales - physiology Xylariales - virology |
| title | Extensive alteration of fungal gene transcript accumulation and elevation of G-protein-regulated cAMP levels by a virulence-attenuating hypovirus |
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