Sensitive and Rapid Detection of Campylobacter Species from Stools of Children with Diarrhea in Japan by the Loop-Mediated Isothermal Amplification Method

We detected Campylobacter spp. in 5% (20/380) of diarrheal stool samples collected at an outpatient clinic in Kyoto using a commercial loop-mediated isothermal amplification (LAMP) kit with a fluorescent detection reagent after DNA extraction. The sensitivity and specificity were 100% in comparison...

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Veröffentlicht in:	Japanese Journal of Infectious Diseases 2014, Vol.67(5), pp.374-378
Hauptverfasser:	Ushijima, Hiroshi, Nishimura, Shuichi, Thongprachum, Aksara, Shimizu-Onda, Yuko, Tran, Dinh Nguyen, Pham, Ngan Thi Kim, Takanashi, Sayaka, Dey, Shuvra Kanti, Okitsu, Shoko, Yamazaki, Wataru, Mizuguchi, Masashi, Hayakawa, Satoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Campylobacter Campylobacter - classification Campylobacter - genetics Campylobacter - isolation & purification Campylobacter Infections - diagnosis Campylobacter Infections - microbiology Child Child, Preschool Coinfection - diagnosis culture method Diarrhea - diagnosis Diarrhea - microbiology Feces - microbiology Female Humans Infant Japan LAMP Male Molecular Diagnostic Techniques - methods Norovirus - isolation & purification Nucleic Acid Amplification Techniques - methods Parechovirus - isolation & purification PCR rapid detection Rotavirus - isolation & purification Sensitivity and Specificity
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