Development of a Multiplex Assay to Measure the Effects of Shipping and Storage Conditions on the Quality of RNA Used in Molecular Assays for Detection of Viral Haemorrhagic Septicemia Virus

In routine diagnostics, real-time reverse transcriptase quantitative PCR (RT-qPCR) has become a powerful method for fish health screening. Collection, transportation, and storage conditions of specimens could dramatically affect their integrity and could consequently affect RT-qPCR test results. In...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of aquatic animal health 2014-09, Vol.26 (3), p.173-180
Hauptverfasser:	Siah, A, Duesund, H, Frisch, K, Nylund, A, McKenzie, P, Saksida, S
Format:	Artikel
Sprache:	eng
Schlagworte:	ambient temperature Animals diagnostic techniques ethanol fish health Food Handling - methods gene expression genes Hemorrhagic Septicemia, Viral - diagnosis ice intraperitoneal injection kidneys messenger RNA Novirhabdovirus - genetics Novirhabdovirus - isolation & purification quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - analysis Salmo salar screening septicemia shipping storage temperature transportation viruses
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!