Inactivation of DNA by β-propiolactone
β-propiolactone (BPL) is an alkylating agent which reacts with many nucleophilic reagents including nucleic acids and proteins. BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as...
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| Veröffentlicht in: | Biologicals 1995-09, Vol.23 (3), p.207-211 |
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| container_title | Biologicals |
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| creator | Perrin, Pierre Morgeaux, Sylvie |
| description | β-propiolactone (BPL) is an alkylating agent which reacts with many nucleophilic reagents including nucleic acids and proteins. BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as well as between the DNA strands in the double helix. Consequently, BPL is widely used for the inactivation of viruses (DNA and RNA viruses). Moreover, it alters the capability of residual/contaminating cell DNA to be used as template by various polymerases. Thus, BPL reduces the risks associated with residual/contaminating cell DNA in biologicals. |
| doi_str_mv | 10.1006/biol.1995.0034 |
| format | Article |
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BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as well as between the DNA strands in the double helix. Consequently, BPL is widely used for the inactivation of viruses (DNA and RNA viruses). Moreover, it alters the capability of residual/contaminating cell DNA to be used as template by various polymerases. Thus, BPL reduces the risks associated with residual/contaminating cell DNA in biologicals.</description><identifier>ISSN: 1045-1056</identifier><identifier>EISSN: 1095-8320</identifier><identifier>DOI: 10.1006/biol.1995.0034</identifier><identifier>PMID: 8527119</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Alkylating Agents - pharmacology ; Alteration ; Animals ; Biological activity ; BPL ; Cell Line ; DNA ; DNA - drug effects ; DNA, Viral - drug effects ; Nucleic Acid Conformation - drug effects ; Propiolactone - pharmacology ; Rabies virus - genetics ; Structure</subject><ispartof>Biologicals, 1995-09, Vol.23 (3), p.207-211</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-d7b3addc550f457ac15bc1de16d0db4544a487e12a7fc8eeb0ac0749974b01323</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/biol.1995.0034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8527119$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perrin, Pierre</creatorcontrib><creatorcontrib>Morgeaux, Sylvie</creatorcontrib><title>Inactivation of DNA by β-propiolactone</title><title>Biologicals</title><addtitle>Biologicals</addtitle><description>β-propiolactone (BPL) is an alkylating agent which reacts with many nucleophilic reagents including nucleic acids and proteins. BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as well as between the DNA strands in the double helix. Consequently, BPL is widely used for the inactivation of viruses (DNA and RNA viruses). Moreover, it alters the capability of residual/contaminating cell DNA to be used as template by various polymerases. Thus, BPL reduces the risks associated with residual/contaminating cell DNA in biologicals.</description><subject>Alkylating Agents - pharmacology</subject><subject>Alteration</subject><subject>Animals</subject><subject>Biological activity</subject><subject>BPL</subject><subject>Cell Line</subject><subject>DNA</subject><subject>DNA - drug effects</subject><subject>DNA, Viral - drug effects</subject><subject>Nucleic Acid Conformation - drug effects</subject><subject>Propiolactone - pharmacology</subject><subject>Rabies virus - genetics</subject><subject>Structure</subject><issn>1045-1056</issn><issn>1095-8320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EKqWwsiFlginhLrHjZKzKV6UKFpgtx75IRmlc4rRS_xY_hN9EolZsTGfpff2c7mHsGiFBgPy-cr5JsCxFApDxEzZFKEVcZCmcjm8uYgSRn7OLED4BELnkEzYpRCoRyym7W7ba9G6ne-fbyNfRw-s8qvbRz3e86fxmgA-xb-mSndW6CXR1nDP28fT4vniJV2_Py8V8FZtMQh9bWWXaWiME1FxIbVBUBi1hbsFWXHCueSEJUy1rUxBVoA1IXpaSV4BZms3Y7YE7bP_aUujV2gVDTaNb8tugUORpLrkcismhaDofQke12nRurbu9QlCjGTWaUaMZNZoZPtwcydtqTfavflQx5MUhp-G8naNOBeOoNWRdR6ZX1rv_0L_amnGg</recordid><startdate>19950901</startdate><enddate>19950901</enddate><creator>Perrin, Pierre</creator><creator>Morgeaux, Sylvie</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19950901</creationdate><title>Inactivation of DNA by β-propiolactone</title><author>Perrin, Pierre ; Morgeaux, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-d7b3addc550f457ac15bc1de16d0db4544a487e12a7fc8eeb0ac0749974b01323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Alkylating Agents - pharmacology</topic><topic>Alteration</topic><topic>Animals</topic><topic>Biological activity</topic><topic>BPL</topic><topic>Cell Line</topic><topic>DNA</topic><topic>DNA - drug effects</topic><topic>DNA, Viral - drug effects</topic><topic>Nucleic Acid Conformation - drug effects</topic><topic>Propiolactone - pharmacology</topic><topic>Rabies virus - genetics</topic><topic>Structure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perrin, Pierre</creatorcontrib><creatorcontrib>Morgeaux, Sylvie</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Biologicals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perrin, Pierre</au><au>Morgeaux, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inactivation of DNA by β-propiolactone</atitle><jtitle>Biologicals</jtitle><addtitle>Biologicals</addtitle><date>1995-09-01</date><risdate>1995</risdate><volume>23</volume><issue>3</issue><spage>207</spage><epage>211</epage><pages>207-211</pages><issn>1045-1056</issn><eissn>1095-8320</eissn><abstract>β-propiolactone (BPL) is an alkylating agent which reacts with many nucleophilic reagents including nucleic acids and proteins. BPL modifies the structure of nucleic acids after reaction mainly with purine residues (notably guanine). It induces nicks in DNA, cross-linking between DNA and proteins as well as between the DNA strands in the double helix. Consequently, BPL is widely used for the inactivation of viruses (DNA and RNA viruses). Moreover, it alters the capability of residual/contaminating cell DNA to be used as template by various polymerases. Thus, BPL reduces the risks associated with residual/contaminating cell DNA in biologicals.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8527119</pmid><doi>10.1006/biol.1995.0034</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1045-1056 |
| ispartof | Biologicals, 1995-09, Vol.23 (3), p.207-211 |
| issn | 1045-1056 1095-8320 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15626747 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkylating Agents - pharmacology Alteration Animals Biological activity BPL Cell Line DNA DNA - drug effects DNA, Viral - drug effects Nucleic Acid Conformation - drug effects Propiolactone - pharmacology Rabies virus - genetics Structure |
| title | Inactivation of DNA by β-propiolactone |
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