Knockout of AtDjB1, a J‐domain protein from Arabidopsis thaliana, alters plant responses to osmotic stress and abscisic acid
AtDjB1 is a member of the Arabidopsis thaliana J‐protein family. AtDjB1 is targeted to the mitochondria and plays a crucial role in A. thaliana heat and oxidative stress resistance. Herein, the role of AtDjB1 in adapting to saline and drought stress was studied in A. thaliana. AtDjB1 expression was...
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| Veröffentlicht in: | Physiologia plantarum 2014-10, Vol.152 (2), p.286-300 |
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| creator | Wang, Xingxing Jia, Ning Zhao, Chunlan Fang, Yulu Lv, Tingting Zhou, Wei Sun, Yongzhen Li, Bing |
| description | AtDjB1 is a member of the Arabidopsis thaliana J‐protein family. AtDjB1 is targeted to the mitochondria and plays a crucial role in A. thaliana heat and oxidative stress resistance. Herein, the role of AtDjB1 in adapting to saline and drought stress was studied in A. thaliana. AtDjB1 expression was induced through salinity, dehydration and abscisic acid (ABA) in young seedlings. Reverse genetic analyses indicate that AtDjB1 is a negative regulator in plant osmotic stress tolerance. Further, AtDjB1 knockout mutant plants (atj1‐1) exhibited greater ABA sensitivity compared with the wild‐type (WT) plants and the mutant lines with a rescued AtDjB1 gene. AtDjB1 gene knockout also altered the expression of several ABA‐responsive genes, which suggests that AtDjB1 is involved in osmotic stress tolerance through its effects on ABA signaling pathways. Moreover, atj1‐1 plants exhibited higher glucose levels and greater glucose sensitivity in the post‐germination development stage. Applying glucose promoted an ABA response in seedlings, and the promotion was more evident in atj1‐1 than WT seedlings. Taken together, higher glucose levels in atj1‐1 plants are likely responsible for the greater ABA sensitivity and increased osmotic stress tolerance. |
| doi_str_mv | 10.1111/ppl.12169 |
| format | Article |
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AtDjB1 is targeted to the mitochondria and plays a crucial role in A. thaliana heat and oxidative stress resistance. Herein, the role of AtDjB1 in adapting to saline and drought stress was studied in A. thaliana. AtDjB1 expression was induced through salinity, dehydration and abscisic acid (ABA) in young seedlings. Reverse genetic analyses indicate that AtDjB1 is a negative regulator in plant osmotic stress tolerance. Further, AtDjB1 knockout mutant plants (atj1‐1) exhibited greater ABA sensitivity compared with the wild‐type (WT) plants and the mutant lines with a rescued AtDjB1 gene. AtDjB1 gene knockout also altered the expression of several ABA‐responsive genes, which suggests that AtDjB1 is involved in osmotic stress tolerance through its effects on ABA signaling pathways. Moreover, atj1‐1 plants exhibited higher glucose levels and greater glucose sensitivity in the post‐germination development stage. Applying glucose promoted an ABA response in seedlings, and the promotion was more evident in atj1‐1 than WT seedlings. Taken together, higher glucose levels in atj1‐1 plants are likely responsible for the greater ABA sensitivity and increased osmotic stress tolerance.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.12169</identifier><identifier>PMID: 24521401</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Abscisic acid ; Abscisic Acid - pharmacology ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Cotyledon - drug effects ; Cotyledon - growth & development ; Droughts ; Gene Expression Regulation, Plant - drug effects ; Gene Knockout Techniques ; gene targeting ; genes ; Genes, Plant ; Germination - drug effects ; glucose ; Glucose - metabolism ; heat ; HSP40 Heat-Shock Proteins - genetics ; HSP40 Heat-Shock Proteins - metabolism ; Mannitol - pharmacology ; Medical research ; mitochondria ; Models, Biological ; mutants ; Mutation - genetics ; Osmosis ; Osmotic Pressure - drug effects ; osmotic stress ; oxidative stress ; plant response ; Protein Structure, Tertiary ; Salinity ; seedlings ; Seedlings - drug effects ; Seedlings - growth & development ; signal transduction ; Sodium Chloride - pharmacology ; stress tolerance ; Stress, Physiological - drug effects ; water stress</subject><ispartof>Physiologia plantarum, 2014-10, Vol.152 (2), p.286-300</ispartof><rights>2014 Scandinavian Plant Physiology Society</rights><rights>2014 Scandinavian Plant Physiology Society.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4859-dad6ce077c081d7da1a20279eb7ee86dfdf5181d249b67f2d3e48a09e6204eab3</citedby><cites>FETCH-LOGICAL-c4859-dad6ce077c081d7da1a20279eb7ee86dfdf5181d249b67f2d3e48a09e6204eab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fppl.12169$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fppl.12169$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24521401$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xingxing</creatorcontrib><creatorcontrib>Jia, Ning</creatorcontrib><creatorcontrib>Zhao, Chunlan</creatorcontrib><creatorcontrib>Fang, Yulu</creatorcontrib><creatorcontrib>Lv, Tingting</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Sun, Yongzhen</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><title>Knockout of AtDjB1, a J‐domain protein from Arabidopsis thaliana, alters plant responses to osmotic stress and abscisic acid</title><title>Physiologia plantarum</title><addtitle>Physiol Plantarum</addtitle><description>AtDjB1 is a member of the Arabidopsis thaliana J‐protein family. AtDjB1 is targeted to the mitochondria and plays a crucial role in A. thaliana heat and oxidative stress resistance. Herein, the role of AtDjB1 in adapting to saline and drought stress was studied in A. thaliana. AtDjB1 expression was induced through salinity, dehydration and abscisic acid (ABA) in young seedlings. Reverse genetic analyses indicate that AtDjB1 is a negative regulator in plant osmotic stress tolerance. Further, AtDjB1 knockout mutant plants (atj1‐1) exhibited greater ABA sensitivity compared with the wild‐type (WT) plants and the mutant lines with a rescued AtDjB1 gene. AtDjB1 gene knockout also altered the expression of several ABA‐responsive genes, which suggests that AtDjB1 is involved in osmotic stress tolerance through its effects on ABA signaling pathways. Moreover, atj1‐1 plants exhibited higher glucose levels and greater glucose sensitivity in the post‐germination development stage. Applying glucose promoted an ABA response in seedlings, and the promotion was more evident in atj1‐1 than WT seedlings. Taken together, higher glucose levels in atj1‐1 plants are likely responsible for the greater ABA sensitivity and increased osmotic stress tolerance.</description><subject>Abscisic acid</subject><subject>Abscisic Acid - pharmacology</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Cotyledon - drug effects</subject><subject>Cotyledon - growth & development</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Gene Knockout Techniques</subject><subject>gene targeting</subject><subject>genes</subject><subject>Genes, Plant</subject><subject>Germination - drug effects</subject><subject>glucose</subject><subject>Glucose - metabolism</subject><subject>heat</subject><subject>HSP40 Heat-Shock Proteins - genetics</subject><subject>HSP40 Heat-Shock Proteins - metabolism</subject><subject>Mannitol - pharmacology</subject><subject>Medical research</subject><subject>mitochondria</subject><subject>Models, Biological</subject><subject>mutants</subject><subject>Mutation - genetics</subject><subject>Osmosis</subject><subject>Osmotic Pressure - drug effects</subject><subject>osmotic stress</subject><subject>oxidative stress</subject><subject>plant response</subject><subject>Protein Structure, Tertiary</subject><subject>Salinity</subject><subject>seedlings</subject><subject>Seedlings - drug effects</subject><subject>Seedlings - growth & development</subject><subject>signal transduction</subject><subject>Sodium Chloride - pharmacology</subject><subject>stress tolerance</subject><subject>Stress, Physiological - drug effects</subject><subject>water stress</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10cFu1DAQBmALgehSOPACYIkLSKS1ncROjtttaYEVVIIKxMWaxA54m8TB4wh6QTwCz8iTYLptD0j4MpL9za-xhpCHnO3xdPanqd_jgsv6FlnwvK6znJXFbbJgLOdZnXO1Q-4hbhjjUnJxl-yIohS8YHxBfrwefXvu50h9R5fxcHPAn1Ogr37__GX8AG6kU_DRptoFP9BlgMYZP6FDGr9A72CE5PtoA9KphzHSYHHyI9oEPPU4-OhaijFdI4XRUGiwdZjuoHXmPrnTQY_2wVXdJWcvjt6vTrL12-OXq-U6a4uqrDMDRraWKdWyihtlgINgQtW2UdZW0nSmK3l6EUXdSNUJk9uiAlZbKVhhocl3ydNtbvrN19li1IPD1vZpYutn1LyUQkqhqirRJ__QjZ_DmKa7VExxIcuknm1VGzxisJ2eghsgXGjO9N-l6LQUfbmUZB9dJc7NYM2NvN5CAvtb8M319uL_Sfr0dH0dmW07HEb7_aYDwrmWKlel_vDmWJ-sxQH79FHoVfKPt74Dr-FzcKjP3gnGS8ZYWbGU-AcMd6-t</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Wang, Xingxing</creator><creator>Jia, Ning</creator><creator>Zhao, Chunlan</creator><creator>Fang, Yulu</creator><creator>Lv, Tingting</creator><creator>Zhou, Wei</creator><creator>Sun, Yongzhen</creator><creator>Li, Bing</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>201410</creationdate><title>Knockout of AtDjB1, a J‐domain protein from Arabidopsis thaliana, alters plant responses to osmotic stress and abscisic acid</title><author>Wang, Xingxing ; Jia, Ning ; Zhao, Chunlan ; Fang, Yulu ; Lv, Tingting ; Zhou, Wei ; Sun, Yongzhen ; Li, Bing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4859-dad6ce077c081d7da1a20279eb7ee86dfdf5181d249b67f2d3e48a09e6204eab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Abscisic acid</topic><topic>Abscisic Acid - pharmacology</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Cotyledon - drug effects</topic><topic>Cotyledon - growth & development</topic><topic>Droughts</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Gene Knockout Techniques</topic><topic>gene targeting</topic><topic>genes</topic><topic>Genes, Plant</topic><topic>Germination - drug effects</topic><topic>glucose</topic><topic>Glucose - metabolism</topic><topic>heat</topic><topic>HSP40 Heat-Shock Proteins - genetics</topic><topic>HSP40 Heat-Shock Proteins - metabolism</topic><topic>Mannitol - pharmacology</topic><topic>Medical research</topic><topic>mitochondria</topic><topic>Models, Biological</topic><topic>mutants</topic><topic>Mutation - genetics</topic><topic>Osmosis</topic><topic>Osmotic Pressure - drug effects</topic><topic>osmotic stress</topic><topic>oxidative stress</topic><topic>plant response</topic><topic>Protein Structure, Tertiary</topic><topic>Salinity</topic><topic>seedlings</topic><topic>Seedlings - drug effects</topic><topic>Seedlings - growth & development</topic><topic>signal transduction</topic><topic>Sodium Chloride - pharmacology</topic><topic>stress tolerance</topic><topic>Stress, Physiological - drug effects</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xingxing</creatorcontrib><creatorcontrib>Jia, Ning</creatorcontrib><creatorcontrib>Zhao, Chunlan</creatorcontrib><creatorcontrib>Fang, Yulu</creatorcontrib><creatorcontrib>Lv, Tingting</creatorcontrib><creatorcontrib>Zhou, Wei</creatorcontrib><creatorcontrib>Sun, Yongzhen</creatorcontrib><creatorcontrib>Li, Bing</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xingxing</au><au>Jia, Ning</au><au>Zhao, Chunlan</au><au>Fang, Yulu</au><au>Lv, Tingting</au><au>Zhou, Wei</au><au>Sun, Yongzhen</au><au>Li, Bing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Knockout of AtDjB1, a J‐domain protein from Arabidopsis thaliana, alters plant responses to osmotic stress and abscisic acid</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plantarum</addtitle><date>2014-10</date><risdate>2014</risdate><volume>152</volume><issue>2</issue><spage>286</spage><epage>300</epage><pages>286-300</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><abstract>AtDjB1 is a member of the Arabidopsis thaliana J‐protein family. AtDjB1 is targeted to the mitochondria and plays a crucial role in A. thaliana heat and oxidative stress resistance. Herein, the role of AtDjB1 in adapting to saline and drought stress was studied in A. thaliana. AtDjB1 expression was induced through salinity, dehydration and abscisic acid (ABA) in young seedlings. Reverse genetic analyses indicate that AtDjB1 is a negative regulator in plant osmotic stress tolerance. Further, AtDjB1 knockout mutant plants (atj1‐1) exhibited greater ABA sensitivity compared with the wild‐type (WT) plants and the mutant lines with a rescued AtDjB1 gene. AtDjB1 gene knockout also altered the expression of several ABA‐responsive genes, which suggests that AtDjB1 is involved in osmotic stress tolerance through its effects on ABA signaling pathways. Moreover, atj1‐1 plants exhibited higher glucose levels and greater glucose sensitivity in the post‐germination development stage. Applying glucose promoted an ABA response in seedlings, and the promotion was more evident in atj1‐1 than WT seedlings. Taken together, higher glucose levels in atj1‐1 plants are likely responsible for the greater ABA sensitivity and increased osmotic stress tolerance.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>24521401</pmid><doi>10.1111/ppl.12169</doi><tpages>15</tpages></addata></record> |
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| subjects | Abscisic acid Abscisic Acid - pharmacology Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Cotyledon - drug effects Cotyledon - growth & development Droughts Gene Expression Regulation, Plant - drug effects Gene Knockout Techniques gene targeting genes Genes, Plant Germination - drug effects glucose Glucose - metabolism heat HSP40 Heat-Shock Proteins - genetics HSP40 Heat-Shock Proteins - metabolism Mannitol - pharmacology Medical research mitochondria Models, Biological mutants Mutation - genetics Osmosis Osmotic Pressure - drug effects osmotic stress oxidative stress plant response Protein Structure, Tertiary Salinity seedlings Seedlings - drug effects Seedlings - growth & development signal transduction Sodium Chloride - pharmacology stress tolerance Stress, Physiological - drug effects water stress |
| title | Knockout of AtDjB1, a J‐domain protein from Arabidopsis thaliana, alters plant responses to osmotic stress and abscisic acid |
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