Methods for the quantification of Frankia cell biomass
Six methods for the estimation of microbial biomass were compared for determination of Frankia cell concentrations. Six strains of Frankia were cultivated in stationary culture, harvested by centrifugation, washed with a saline buffer and diluted to five standardized concentrations. These cell suspe...
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| Veröffentlicht in: | Plant and soil 1989-08, Vol.118 (1), p.199-204 |
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| container_title | Plant and soil |
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| creator | Nittayajarn, A. (Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand)) Baker, D.D |
| description | Six methods for the estimation of microbial biomass were compared for determination of Frankia cell concentrations. Six strains of Frankia were cultivated in stationary culture, harvested by centrifugation, washed with a saline buffer and diluted to five standardized concentrations. These cell suspensions were then used to assess reliability of each of the biomass determination methods. The destructive total protein determination methods were the most sensitive and reliable. Two non-destructive methods, packed cell volume and turbidity measurement, were also accurate, and because of their simplicity hold advantage for routine growth measurements and inoculum dilutions. Dry weight determinations were inconsistent for the small cell masses used in this study. An ELISA procedure demonstrated reliability but little sensitivity. |
| doi_str_mv | 10.1007/BF02232807 |
| format | Article |
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(Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand)) ; Baker, D.D</creator><creatorcontrib>Nittayajarn, A. (Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand)) ; Baker, D.D</creatorcontrib><description>Six methods for the estimation of microbial biomass were compared for determination of Frankia cell concentrations. Six strains of Frankia were cultivated in stationary culture, harvested by centrifugation, washed with a saline buffer and diluted to five standardized concentrations. These cell suspensions were then used to assess reliability of each of the biomass determination methods. The destructive total protein determination methods were the most sensitive and reliable. Two non-destructive methods, packed cell volume and turbidity measurement, were also accurate, and because of their simplicity hold advantage for routine growth measurements and inoculum dilutions. Dry weight determinations were inconsistent for the small cell masses used in this study. An ELISA procedure demonstrated reliability but little sensitivity.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/BF02232807</identifier><identifier>CODEN: PLSOA2</identifier><language>eng</language><publisher>Dordrecht: Kluwer Academic Publishers</publisher><subject>Agronomy. Soil science and plant productions ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; BIOMASA ; BIOMASS ; BIOMASSE ; Centrifugation ; Chemical suspensions ; Economic plant physiology ; Enzyme linked immunosorbent assay ; FRANKIA ; Fundamental and applied biological sciences. Psychology ; Hematocrit ; Homogenization ; Microbiology ; MICROORGANISME ; MICROORGANISMOS ; MICROORGANISMS ; Plants ; Quantification ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; Turbidity</subject><ispartof>Plant and soil, 1989-08, Vol.118 (1), p.199-204</ispartof><rights>1989 Kluwer Academic Publishers</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42938235$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42938235$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,799,23909,23910,25118,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6628858$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Nittayajarn, A. (Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand))</creatorcontrib><creatorcontrib>Baker, D.D</creatorcontrib><title>Methods for the quantification of Frankia cell biomass</title><title>Plant and soil</title><description>Six methods for the estimation of microbial biomass were compared for determination of Frankia cell concentrations. Six strains of Frankia were cultivated in stationary culture, harvested by centrifugation, washed with a saline buffer and diluted to five standardized concentrations. These cell suspensions were then used to assess reliability of each of the biomass determination methods. The destructive total protein determination methods were the most sensitive and reliable. Two non-destructive methods, packed cell volume and turbidity measurement, were also accurate, and because of their simplicity hold advantage for routine growth measurements and inoculum dilutions. Dry weight determinations were inconsistent for the small cell masses used in this study. An ELISA procedure demonstrated reliability but little sensitivity.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>BIOMASA</subject><subject>BIOMASS</subject><subject>BIOMASSE</subject><subject>Centrifugation</subject><subject>Chemical suspensions</subject><subject>Economic plant physiology</subject><subject>Enzyme linked immunosorbent assay</subject><subject>FRANKIA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hematocrit</subject><subject>Homogenization</subject><subject>Microbiology</subject><subject>MICROORGANISME</subject><subject>MICROORGANISMOS</subject><subject>MICROORGANISMS</subject><subject>Plants</subject><subject>Quantification</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>Turbidity</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNo9js1Lw0AQRxdRsFYvHgUhB_EWnf3eHLVYFaJeFLyF6WbXbk2zbTY9-N-b2uJpGN7j8SPknMINBdC391NgjDMD-oCMqNQ8l8DVIRkBcJaDLj6PyUlKC9j-VI2IenH9PNYp87HL-rnL1hts--CDxT7ENos-m3bYfgfMrGuabBbiElM6JUcem-TO9ndMPqYP75OnvHx7fJ7clblnUvR57YysEbwFJ0DR2lArNLV2JhSCVNxzp5zyuqhNrb2nQlhmjOVg3UxqRD4m17vuqovrjUt9tQxpOwRbFzepolIxCdIM4tVexGSx8cNmG1K16sISu59KqaH7p13utEXqY_ePBSu4YVwO_GLHPcYKv7oh8VqaAkTBC_4Lw5Vmpg</recordid><startdate>19890801</startdate><enddate>19890801</enddate><creator>Nittayajarn, A. 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Soil science and plant productions</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>BIOMASA</topic><topic>BIOMASS</topic><topic>BIOMASSE</topic><topic>Centrifugation</topic><topic>Chemical suspensions</topic><topic>Economic plant physiology</topic><topic>Enzyme linked immunosorbent assay</topic><topic>FRANKIA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hematocrit</topic><topic>Homogenization</topic><topic>Microbiology</topic><topic>MICROORGANISME</topic><topic>MICROORGANISMOS</topic><topic>MICROORGANISMS</topic><topic>Plants</topic><topic>Quantification</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>Turbidity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nittayajarn, A. (Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand))</creatorcontrib><creatorcontrib>Baker, D.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nittayajarn, A. (Biological Nitrogen Fixation Resource Centre for Southeast Asia, Bangkok (Thailand))</au><au>Baker, D.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methods for the quantification of Frankia cell biomass</atitle><jtitle>Plant and soil</jtitle><date>1989-08-01</date><risdate>1989</risdate><volume>118</volume><issue>1</issue><spage>199</spage><epage>204</epage><pages>199-204</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><coden>PLSOA2</coden><abstract>Six methods for the estimation of microbial biomass were compared for determination of Frankia cell concentrations. Six strains of Frankia were cultivated in stationary culture, harvested by centrifugation, washed with a saline buffer and diluted to five standardized concentrations. These cell suspensions were then used to assess reliability of each of the biomass determination methods. The destructive total protein determination methods were the most sensitive and reliable. Two non-destructive methods, packed cell volume and turbidity measurement, were also accurate, and because of their simplicity hold advantage for routine growth measurements and inoculum dilutions. Dry weight determinations were inconsistent for the small cell masses used in this study. An ELISA procedure demonstrated reliability but little sensitivity.</abstract><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><doi>10.1007/BF02232807</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0032-079X |
| ispartof | Plant and soil, 1989-08, Vol.118 (1), p.199-204 |
| issn | 0032-079X 1573-5036 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15625058 |
| source | Jstor Complete Legacy; SpringerLink Journals - AutoHoldings |
| subjects | Agronomy. Soil science and plant productions Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences BIOMASA BIOMASS BIOMASSE Centrifugation Chemical suspensions Economic plant physiology Enzyme linked immunosorbent assay FRANKIA Fundamental and applied biological sciences. Psychology Hematocrit Homogenization Microbiology MICROORGANISME MICROORGANISMOS MICROORGANISMS Plants Quantification Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Turbidity |
| title | Methods for the quantification of Frankia cell biomass |
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