Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process
•Normal human melanocytes (NHMs) migrated to form cell clusters on Matrigel.•The melanin synthesis is significantly decreased in melanocyte clusters on Matrigel.•The PAX3 level of melanocyte clusters on Matrigel is upregulated.•The expression profile of PAX3, SOX10, and MITF in the cluster is simila...
Gespeichert in:
| Veröffentlicht in: | Journal of dermatological science 2014-10, Vol.76 (1), p.60-66 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 66 |
|---|---|
| container_issue | 1 |
| container_start_page | 60 |
| container_title | Journal of dermatological science |
| container_volume | 76 |
| creator | Choi, Hyunjung Jin, Sun Hee Han, Mi Hwa Lee, Jinyoung Ahn, Seyeon Seong, Minjeong Choi, Hyun Han, Jiyeon Cho, Eun-Gyung Lee, Tae Ryong Noh, Minsoo |
| description | •Normal human melanocytes (NHMs) migrated to form cell clusters on Matrigel.•The melanin synthesis is significantly decreased in melanocyte clusters on Matrigel.•The PAX3 level of melanocyte clusters on Matrigel is upregulated.•The expression profile of PAX3, SOX10, and MITF in the cluster is similar to that of melanoblasts.
The interactions between human epidermal melanocytes and their cellular microenvironment are important in the regulation of human melanocyte functions or in their malignant transformation into melanoma. Although the basement membrane extracellular matrix (BM-ECM) is one of major melanocyte microenvironments, the effects of BM-ECM on the human melanocyte functions are not fully explained at a molecular level.
This study was aimed to characterize the molecular and cellular interactions between normal human melanocytes (NHMs) and BM-ECM.
We investigated cell culture models of normal human melanocytes or melanoma cells on three-dimensional (3D) Matrigel to understand the roles of the basement membrane microenvironment in human melanocyte functions. Melanogenesis and melanobast biomarker expression in both primary human melanocytes and melanoma cells on 3D Matrigel were evaluated.
We found that NHMs migrated and formed reversible paired box 3 (PAX3) expressing cell clusters on three-dimensional (3D) Matrigel. The melanogenesis was significantly decreased in the PAX3 expressing cell cluster. The expression profile of PAX3, SOX10, and MITF in the melanocyte cluster on 3D Matrigel was similar to that of melanoblasts. Interestingly, PAX3 and SOX10 showed an inverse expression profile in NHMs, whereas the inverse expression pattern of PAX3 and SOX10 was disrupted in melanoma MNT1 and WM266-4 cells.
The human melanocyte culture on 3D Matrigel provides an alternative model system to study functions of human melanoblasts. In addition, this system will contribute to the elucidation of PAX3-related tumorigenic mechanisms to understand human melanoma. |
| doi_str_mv | 10.1016/j.jdermsci.2014.07.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1562441919</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0923181114001625</els_id><sourcerecordid>1562441919</sourcerecordid><originalsourceid>FETCH-LOGICAL-c517t-888d10d04b96173ccc472b28e21a00d9eb9d7db5730cc8af1714407b91b41e9b3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EokvhL1Q-ckmYcZw4viCqqlCkoiK1SL1ZjjO7OORjsRPE_nscbYsQl558mPfD8wxjZwg5AlbvurxrKQzR-VwAyhxUDlA9YxusVZGVlb5_zjagRZFhjXjCXsXYAUAppH7JTkSJota13DC6WgY78oF6O07uMFPk2ykM3PKv5_dFRr_3gWL04-4fCXf9EmcKfBr5FzsHv6OeNwc-f08j6ns--F2ws0_jfZhc8r9mL7a2j_Tm4T1l3z5e3l1cZdc3nz5fnF9nrkQ1Z3VdtwgtyEZXqArnnFSiETUJtACtpka3qm1KVYBztd2iQilBNRobiaSb4pS9Peam3p8LxdkMPq5fsiNNSzRYVkJK1KiTtDpKXZhiDLQ1--AHGw4GwayITWceEZsVsQFlEuJkPHvoWJqB2r-2R6ZJ8OEooLTpL0_BpAgaHbU-kJtNO_mnO97_F-F6P3pn-x90oNhNSxgTR4MmCgPmdj30emeUkFJFWfwBJVOmEQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1562441919</pqid></control><display><type>article</type><title>Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Choi, Hyunjung ; Jin, Sun Hee ; Han, Mi Hwa ; Lee, Jinyoung ; Ahn, Seyeon ; Seong, Minjeong ; Choi, Hyun ; Han, Jiyeon ; Cho, Eun-Gyung ; Lee, Tae Ryong ; Noh, Minsoo</creator><creatorcontrib>Choi, Hyunjung ; Jin, Sun Hee ; Han, Mi Hwa ; Lee, Jinyoung ; Ahn, Seyeon ; Seong, Minjeong ; Choi, Hyun ; Han, Jiyeon ; Cho, Eun-Gyung ; Lee, Tae Ryong ; Noh, Minsoo</creatorcontrib><description>•Normal human melanocytes (NHMs) migrated to form cell clusters on Matrigel.•The melanin synthesis is significantly decreased in melanocyte clusters on Matrigel.•The PAX3 level of melanocyte clusters on Matrigel is upregulated.•The expression profile of PAX3, SOX10, and MITF in the cluster is similar to that of melanoblasts.
The interactions between human epidermal melanocytes and their cellular microenvironment are important in the regulation of human melanocyte functions or in their malignant transformation into melanoma. Although the basement membrane extracellular matrix (BM-ECM) is one of major melanocyte microenvironments, the effects of BM-ECM on the human melanocyte functions are not fully explained at a molecular level.
This study was aimed to characterize the molecular and cellular interactions between normal human melanocytes (NHMs) and BM-ECM.
We investigated cell culture models of normal human melanocytes or melanoma cells on three-dimensional (3D) Matrigel to understand the roles of the basement membrane microenvironment in human melanocyte functions. Melanogenesis and melanobast biomarker expression in both primary human melanocytes and melanoma cells on 3D Matrigel were evaluated.
We found that NHMs migrated and formed reversible paired box 3 (PAX3) expressing cell clusters on three-dimensional (3D) Matrigel. The melanogenesis was significantly decreased in the PAX3 expressing cell cluster. The expression profile of PAX3, SOX10, and MITF in the melanocyte cluster on 3D Matrigel was similar to that of melanoblasts. Interestingly, PAX3 and SOX10 showed an inverse expression profile in NHMs, whereas the inverse expression pattern of PAX3 and SOX10 was disrupted in melanoma MNT1 and WM266-4 cells.
The human melanocyte culture on 3D Matrigel provides an alternative model system to study functions of human melanoblasts. In addition, this system will contribute to the elucidation of PAX3-related tumorigenic mechanisms to understand human melanoma.</description><identifier>ISSN: 0923-1811</identifier><identifier>EISSN: 1873-569X</identifier><identifier>DOI: 10.1016/j.jdermsci.2014.07.006</identifier><identifier>PMID: 25128984</identifier><language>eng</language><publisher>Netherlands: Elsevier Ireland Ltd</publisher><subject>Basement Membrane - metabolism ; Cell Line, Tumor ; Cell Movement ; Collagen - chemistry ; Dermatology ; Drug Combinations ; Extracellular Matrix - metabolism ; Gene Expression Profiling ; Human melanocytes ; Humans ; Laminin - chemistry ; Matrigel ; Melanins - chemistry ; Melanoblast ; Melanocyte cluster ; Melanocytes - cytology ; Melanocytes - metabolism ; Melanoma ; Melanoma - metabolism ; Microphthalmia-Associated Transcription Factor - metabolism ; Paired Box Transcription Factors - metabolism ; PAX3 ; PAX3 Transcription Factor ; Pigmentation ; Proteoglycans - chemistry ; SOXE Transcription Factors - metabolism ; Up-Regulation</subject><ispartof>Journal of dermatological science, 2014-10, Vol.76 (1), p.60-66</ispartof><rights>Japanese Society for Investigative Dermatology</rights><rights>2014 Japanese Society for Investigative Dermatology</rights><rights>Copyright © 2014 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-888d10d04b96173ccc472b28e21a00d9eb9d7db5730cc8af1714407b91b41e9b3</citedby><cites>FETCH-LOGICAL-c517t-888d10d04b96173ccc472b28e21a00d9eb9d7db5730cc8af1714407b91b41e9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0923181114001625$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25128984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Hyunjung</creatorcontrib><creatorcontrib>Jin, Sun Hee</creatorcontrib><creatorcontrib>Han, Mi Hwa</creatorcontrib><creatorcontrib>Lee, Jinyoung</creatorcontrib><creatorcontrib>Ahn, Seyeon</creatorcontrib><creatorcontrib>Seong, Minjeong</creatorcontrib><creatorcontrib>Choi, Hyun</creatorcontrib><creatorcontrib>Han, Jiyeon</creatorcontrib><creatorcontrib>Cho, Eun-Gyung</creatorcontrib><creatorcontrib>Lee, Tae Ryong</creatorcontrib><creatorcontrib>Noh, Minsoo</creatorcontrib><title>Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process</title><title>Journal of dermatological science</title><addtitle>J Dermatol Sci</addtitle><description>•Normal human melanocytes (NHMs) migrated to form cell clusters on Matrigel.•The melanin synthesis is significantly decreased in melanocyte clusters on Matrigel.•The PAX3 level of melanocyte clusters on Matrigel is upregulated.•The expression profile of PAX3, SOX10, and MITF in the cluster is similar to that of melanoblasts.
The interactions between human epidermal melanocytes and their cellular microenvironment are important in the regulation of human melanocyte functions or in their malignant transformation into melanoma. Although the basement membrane extracellular matrix (BM-ECM) is one of major melanocyte microenvironments, the effects of BM-ECM on the human melanocyte functions are not fully explained at a molecular level.
This study was aimed to characterize the molecular and cellular interactions between normal human melanocytes (NHMs) and BM-ECM.
We investigated cell culture models of normal human melanocytes or melanoma cells on three-dimensional (3D) Matrigel to understand the roles of the basement membrane microenvironment in human melanocyte functions. Melanogenesis and melanobast biomarker expression in both primary human melanocytes and melanoma cells on 3D Matrigel were evaluated.
We found that NHMs migrated and formed reversible paired box 3 (PAX3) expressing cell clusters on three-dimensional (3D) Matrigel. The melanogenesis was significantly decreased in the PAX3 expressing cell cluster. The expression profile of PAX3, SOX10, and MITF in the melanocyte cluster on 3D Matrigel was similar to that of melanoblasts. Interestingly, PAX3 and SOX10 showed an inverse expression profile in NHMs, whereas the inverse expression pattern of PAX3 and SOX10 was disrupted in melanoma MNT1 and WM266-4 cells.
The human melanocyte culture on 3D Matrigel provides an alternative model system to study functions of human melanoblasts. In addition, this system will contribute to the elucidation of PAX3-related tumorigenic mechanisms to understand human melanoma.</description><subject>Basement Membrane - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement</subject><subject>Collagen - chemistry</subject><subject>Dermatology</subject><subject>Drug Combinations</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gene Expression Profiling</subject><subject>Human melanocytes</subject><subject>Humans</subject><subject>Laminin - chemistry</subject><subject>Matrigel</subject><subject>Melanins - chemistry</subject><subject>Melanoblast</subject><subject>Melanocyte cluster</subject><subject>Melanocytes - cytology</subject><subject>Melanocytes - metabolism</subject><subject>Melanoma</subject><subject>Melanoma - metabolism</subject><subject>Microphthalmia-Associated Transcription Factor - metabolism</subject><subject>Paired Box Transcription Factors - metabolism</subject><subject>PAX3</subject><subject>PAX3 Transcription Factor</subject><subject>Pigmentation</subject><subject>Proteoglycans - chemistry</subject><subject>SOXE Transcription Factors - metabolism</subject><subject>Up-Regulation</subject><issn>0923-1811</issn><issn>1873-569X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhL1Q-ckmYcZw4viCqqlCkoiK1SL1ZjjO7OORjsRPE_nscbYsQl558mPfD8wxjZwg5AlbvurxrKQzR-VwAyhxUDlA9YxusVZGVlb5_zjagRZFhjXjCXsXYAUAppH7JTkSJota13DC6WgY78oF6O07uMFPk2ykM3PKv5_dFRr_3gWL04-4fCXf9EmcKfBr5FzsHv6OeNwc-f08j6ns--F2ws0_jfZhc8r9mL7a2j_Tm4T1l3z5e3l1cZdc3nz5fnF9nrkQ1Z3VdtwgtyEZXqArnnFSiETUJtACtpka3qm1KVYBztd2iQilBNRobiaSb4pS9Peam3p8LxdkMPq5fsiNNSzRYVkJK1KiTtDpKXZhiDLQ1--AHGw4GwayITWceEZsVsQFlEuJkPHvoWJqB2r-2R6ZJ8OEooLTpL0_BpAgaHbU-kJtNO_mnO97_F-F6P3pn-x90oNhNSxgTR4MmCgPmdj30emeUkFJFWfwBJVOmEQ</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Choi, Hyunjung</creator><creator>Jin, Sun Hee</creator><creator>Han, Mi Hwa</creator><creator>Lee, Jinyoung</creator><creator>Ahn, Seyeon</creator><creator>Seong, Minjeong</creator><creator>Choi, Hyun</creator><creator>Han, Jiyeon</creator><creator>Cho, Eun-Gyung</creator><creator>Lee, Tae Ryong</creator><creator>Noh, Minsoo</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process</title><author>Choi, Hyunjung ; Jin, Sun Hee ; Han, Mi Hwa ; Lee, Jinyoung ; Ahn, Seyeon ; Seong, Minjeong ; Choi, Hyun ; Han, Jiyeon ; Cho, Eun-Gyung ; Lee, Tae Ryong ; Noh, Minsoo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-888d10d04b96173ccc472b28e21a00d9eb9d7db5730cc8af1714407b91b41e9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Basement Membrane - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement</topic><topic>Collagen - chemistry</topic><topic>Dermatology</topic><topic>Drug Combinations</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gene Expression Profiling</topic><topic>Human melanocytes</topic><topic>Humans</topic><topic>Laminin - chemistry</topic><topic>Matrigel</topic><topic>Melanins - chemistry</topic><topic>Melanoblast</topic><topic>Melanocyte cluster</topic><topic>Melanocytes - cytology</topic><topic>Melanocytes - metabolism</topic><topic>Melanoma</topic><topic>Melanoma - metabolism</topic><topic>Microphthalmia-Associated Transcription Factor - metabolism</topic><topic>Paired Box Transcription Factors - metabolism</topic><topic>PAX3</topic><topic>PAX3 Transcription Factor</topic><topic>Pigmentation</topic><topic>Proteoglycans - chemistry</topic><topic>SOXE Transcription Factors - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Hyunjung</creatorcontrib><creatorcontrib>Jin, Sun Hee</creatorcontrib><creatorcontrib>Han, Mi Hwa</creatorcontrib><creatorcontrib>Lee, Jinyoung</creatorcontrib><creatorcontrib>Ahn, Seyeon</creatorcontrib><creatorcontrib>Seong, Minjeong</creatorcontrib><creatorcontrib>Choi, Hyun</creatorcontrib><creatorcontrib>Han, Jiyeon</creatorcontrib><creatorcontrib>Cho, Eun-Gyung</creatorcontrib><creatorcontrib>Lee, Tae Ryong</creatorcontrib><creatorcontrib>Noh, Minsoo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dermatological science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Hyunjung</au><au>Jin, Sun Hee</au><au>Han, Mi Hwa</au><au>Lee, Jinyoung</au><au>Ahn, Seyeon</au><au>Seong, Minjeong</au><au>Choi, Hyun</au><au>Han, Jiyeon</au><au>Cho, Eun-Gyung</au><au>Lee, Tae Ryong</au><au>Noh, Minsoo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process</atitle><jtitle>Journal of dermatological science</jtitle><addtitle>J Dermatol Sci</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>76</volume><issue>1</issue><spage>60</spage><epage>66</epage><pages>60-66</pages><issn>0923-1811</issn><eissn>1873-569X</eissn><abstract>•Normal human melanocytes (NHMs) migrated to form cell clusters on Matrigel.•The melanin synthesis is significantly decreased in melanocyte clusters on Matrigel.•The PAX3 level of melanocyte clusters on Matrigel is upregulated.•The expression profile of PAX3, SOX10, and MITF in the cluster is similar to that of melanoblasts.
The interactions between human epidermal melanocytes and their cellular microenvironment are important in the regulation of human melanocyte functions or in their malignant transformation into melanoma. Although the basement membrane extracellular matrix (BM-ECM) is one of major melanocyte microenvironments, the effects of BM-ECM on the human melanocyte functions are not fully explained at a molecular level.
This study was aimed to characterize the molecular and cellular interactions between normal human melanocytes (NHMs) and BM-ECM.
We investigated cell culture models of normal human melanocytes or melanoma cells on three-dimensional (3D) Matrigel to understand the roles of the basement membrane microenvironment in human melanocyte functions. Melanogenesis and melanobast biomarker expression in both primary human melanocytes and melanoma cells on 3D Matrigel were evaluated.
We found that NHMs migrated and formed reversible paired box 3 (PAX3) expressing cell clusters on three-dimensional (3D) Matrigel. The melanogenesis was significantly decreased in the PAX3 expressing cell cluster. The expression profile of PAX3, SOX10, and MITF in the melanocyte cluster on 3D Matrigel was similar to that of melanoblasts. Interestingly, PAX3 and SOX10 showed an inverse expression profile in NHMs, whereas the inverse expression pattern of PAX3 and SOX10 was disrupted in melanoma MNT1 and WM266-4 cells.
The human melanocyte culture on 3D Matrigel provides an alternative model system to study functions of human melanoblasts. In addition, this system will contribute to the elucidation of PAX3-related tumorigenic mechanisms to understand human melanoma.</abstract><cop>Netherlands</cop><pub>Elsevier Ireland Ltd</pub><pmid>25128984</pmid><doi>10.1016/j.jdermsci.2014.07.006</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-1811 |
| ispartof | Journal of dermatological science, 2014-10, Vol.76 (1), p.60-66 |
| issn | 0923-1811 1873-569X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1562441919 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Basement Membrane - metabolism Cell Line, Tumor Cell Movement Collagen - chemistry Dermatology Drug Combinations Extracellular Matrix - metabolism Gene Expression Profiling Human melanocytes Humans Laminin - chemistry Matrigel Melanins - chemistry Melanoblast Melanocyte cluster Melanocytes - cytology Melanocytes - metabolism Melanoma Melanoma - metabolism Microphthalmia-Associated Transcription Factor - metabolism Paired Box Transcription Factors - metabolism PAX3 PAX3 Transcription Factor Pigmentation Proteoglycans - chemistry SOXE Transcription Factors - metabolism Up-Regulation |
| title | Human melanocytes form a PAX3-expressing melanocyte cluster on Matrigel by the cell migration process |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T14%3A06%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20melanocytes%20form%20a%20PAX3-expressing%20melanocyte%20cluster%20on%20Matrigel%20by%20the%20cell%20migration%20process&rft.jtitle=Journal%20of%20dermatological%20science&rft.au=Choi,%20Hyunjung&rft.date=2014-10-01&rft.volume=76&rft.issue=1&rft.spage=60&rft.epage=66&rft.pages=60-66&rft.issn=0923-1811&rft.eissn=1873-569X&rft_id=info:doi/10.1016/j.jdermsci.2014.07.006&rft_dat=%3Cproquest_cross%3E1562441919%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1562441919&rft_id=info:pmid/25128984&rft_els_id=S0923181114001625&rfr_iscdi=true |