B-cell epitope of beta toxin of Clostridium perfringens genetically conjugated to a carrier protein: Expression, purification and characterization of the chimeric protein

•The B-cell epitope of beta toxin fused with a carrier protein was analyzed.•Three stretches of amino acids were predicted as putative B-cell epitopes.•We obtained high expression of the recombinant fusion protein rLTB-Btx140–156.•The protein retained native LTB properties and exhibited antigenicity...

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Veröffentlicht in:	Protein expression and purification 2014-10, Vol.102, p.38-44
Hauptverfasser:	Bhatia, Bharti, Solanki, Amit Kumar, Kaushik, Himani, Dixit, Aparna, Garg, Lalit C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Toxins - genetics Bacterial Toxins - isolation & purification Bacterial Vaccines - genetics Bacterial Vaccines - isolation & purification Base Sequence Beta toxin Clostridium perfringens Clostridium perfringens - genetics Enterotoxins - genetics Enterotoxins - isolation & purification Epitope Epitope prediction algorithms Epitopes, B-Lymphocyte - genetics Epitopes, B-Lymphocyte - isolation & purification Escherichia coli - genetics Escherichia coli Proteins - genetics Escherichia coli Proteins - isolation & purification Fusion protein LTB Molecular Sequence Data Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Vaccine Vibrio cholerae - genetics
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