Axenic mass cultivation of the free-living soil amoeba, Acanthamoeba castellanii in a laboratory fermentor

Axenic mass cultivation of Acanthamoeba castellanii in laboratory fermentors (141) yielded after 20 days approximately 3 g cells (wet weight). After a short lag phase amoebal cell numbers increased exponentially to a maximum of 3.5 x 10(5) cells per ml until cell death occurred after 20 days. Optica...

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Veröffentlicht in:	Antonie van Leeuwenhoek 1996-05, Vol.69 (4), p.317-322
Hauptverfasser:	WEEKERS, P. H. H, WIJEN, J. P. H, LOMANS, B. P, VOGELS, G. D
Format:	Artikel
Sprache:	eng
Schlagworte:	Acanthamoeba - growth & development Acanthamoeba - metabolism Acanthamoeba castellanii Ammonia - metabolism Ammonia - pharmacology Animals Biological and medical sciences Biotechnology Fermentation Fundamental and applied biological sciences. Psychology Glucose - metabolism Hydrogen-Ion Concentration Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology Oxygen - metabolism Soil
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