Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate
A colorimetric method for determination of lignin peroxidase activity has been developed which is based on the demethylation of methylene blue. The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme...
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| Veröffentlicht in: | Biotechnology techniques 1996, Vol.10 (4), p.273-276 |
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| container_end_page | 276 |
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| container_issue | 4 |
| container_start_page | 273 |
| container_title | Biotechnology techniques |
| container_volume | 10 |
| creator | Magalhaes, D.B Carvalho, M.E.A. de Bon, E Neto, J.S.A Kling, S.H |
| description | A colorimetric method for determination of lignin peroxidase activity has been developed which is based on the demethylation of methylene blue. The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme quantification as its sensitivity is close to the veratryl alcohol assay. |
| doi_str_mv | 10.1007/BF00184028 |
| format | Article |
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The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme quantification as its sensitivity is close to the veratryl alcohol assay.</description><identifier>ISSN: 0951-208X</identifier><identifier>EISSN: 1573-6784</identifier><identifier>DOI: 10.1007/BF00184028</identifier><identifier>CODEN: BTECE6</identifier><language>eng</language><publisher>London: Chapman and Hall</publisher><subject>assays ; Biological and medical sciences ; Biotechnology ; colorimetry ; enzyme activity ; Enzyme engineering ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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The use of this dye shows the practical advantage of using a wavelength in the visible region of spectrum. The method can be efficiently used for the enzyme quantification as its sensitivity is close to the veratryl alcohol assay.</description><subject>assays</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>colorimetry</subject><subject>enzyme activity</subject><subject>Enzyme engineering</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Methods. Procedures. 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| fulltext | fulltext |
| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1996, Vol.10 (4), p.273-276 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15621202 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | assays Biological and medical sciences Biotechnology colorimetry enzyme activity Enzyme engineering Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies methylene blue Miscellaneous peroxidases Phanerochaete chrysosporium |
| title | Colorimetric assay for lignin peroxidase activity determination using methylene blue as substrate |
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