Identification of proteins associated with lymph node metastasis of gastric cancer
Purpose The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins. Methods Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellul...
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| Veröffentlicht in: | Journal of cancer research and clinical oncology 2014-10, Vol.140 (10), p.1739-1749 |
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| creator | Ma, Yan Li, Yan-Feng Wang, Tie Pang, Rui Xue, Ying-Wei Zhao, Shu-Peng |
| description | Purpose
The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins.
Methods
Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellular proteins were separated by two-dimensional gel electrophoresis (2-DE), analyzed by MALDI/TOF–TOF MS, and identified by a database search. Expression of 14-3-3β and profilin-1 was then immunohistochemically verified in paraffin-embedded gastric cancer tissues from 128 patients and analyzed by multivariate logistic regression models, Kaplan–Meier curves, and Cox proportional hazard models.
Results
A total of 26 differentially expressed proteins were identified, 20 of which were overexpressed and 6 of which were underexpressed. 14-3-3β and profilin-1 were upregulated in gastric cancer tissues with and without lymph node metastasis, respectively. Expression of 14-3-3β protein was associated, but profilin-1 expression was inversely associated with gastric cancer lymph node metastasis. Multivariate analysis showed that overexpression of 14-3-3β and reduced expression of profilin-1 were independent risk factors for gastric cancer lymph node metastasis, while 14-3-3β overexpression was an independent prognostic factor for gastric cancer patients.
Conclusions
The current study identified 26 differentially expressed proteins. Further studies showed that both 14-3-3β and profilin-1 protein may be useful biomarkers for prediction of gastric cancer lymph node metastasis and that expression of 14-3-3β was a prognostic marker for gastric cancer patients. |
| doi_str_mv | 10.1007/s00432-014-1679-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1561968479</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1561968479</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-3f608a369932d86a8b06a56073432be7f72e5e6222899efc3e7ff5cfb9362d093</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhYMotlZ_gBuZpZvRPGaSyVKKLygIouuQydy0KfOoSYr035thqkshcJN7zznkfghdE3xHMBb3AeOC0RyTIidcyJyeoDkZO4Sx8hTNMREkLynhM3QRwhandynoOZrRoqIVLeUcvb820EdnndHRDX022GznhwiuD5kOYTBOR2iybxc3WXvodpusHxrIOog6pOPC6Finu3cmM7o34C_RmdVtgKtjXaDPp8eP5Uu-ent-XT6scsOEiDmzHFeacSkZbSquqxpzXXIsWNqgBmEFhRI4pbSSEqxhqWVLY2vJOG2wZAt0O-WmD3_tIUTVuWCgbXUPwz4oUnIieVWIUUomqfFDCB6s2nnXaX9QBKsRpZpQqoRSjSgVTZ6bY_y-7qD5c_yySwI6CUIa9WvwajvsfZ9W_if1BzeHfsc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1561968479</pqid></control><display><type>article</type><title>Identification of proteins associated with lymph node metastasis of gastric cancer</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Ma, Yan ; Li, Yan-Feng ; Wang, Tie ; Pang, Rui ; Xue, Ying-Wei ; Zhao, Shu-Peng</creator><creatorcontrib>Ma, Yan ; Li, Yan-Feng ; Wang, Tie ; Pang, Rui ; Xue, Ying-Wei ; Zhao, Shu-Peng</creatorcontrib><description>Purpose
The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins.
Methods
Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellular proteins were separated by two-dimensional gel electrophoresis (2-DE), analyzed by MALDI/TOF–TOF MS, and identified by a database search. Expression of 14-3-3β and profilin-1 was then immunohistochemically verified in paraffin-embedded gastric cancer tissues from 128 patients and analyzed by multivariate logistic regression models, Kaplan–Meier curves, and Cox proportional hazard models.
Results
A total of 26 differentially expressed proteins were identified, 20 of which were overexpressed and 6 of which were underexpressed. 14-3-3β and profilin-1 were upregulated in gastric cancer tissues with and without lymph node metastasis, respectively. Expression of 14-3-3β protein was associated, but profilin-1 expression was inversely associated with gastric cancer lymph node metastasis. Multivariate analysis showed that overexpression of 14-3-3β and reduced expression of profilin-1 were independent risk factors for gastric cancer lymph node metastasis, while 14-3-3β overexpression was an independent prognostic factor for gastric cancer patients.
Conclusions
The current study identified 26 differentially expressed proteins. Further studies showed that both 14-3-3β and profilin-1 protein may be useful biomarkers for prediction of gastric cancer lymph node metastasis and that expression of 14-3-3β was a prognostic marker for gastric cancer patients.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-014-1679-2</identifier><identifier>PMID: 24828259</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>14-3-3 Proteins - analysis ; Adult ; Aged ; Biomarkers, Tumor - analysis ; Cancer Research ; Down-Regulation ; Electrophoresis, Gel, Two-Dimensional ; Female ; Gastrectomy ; Gene Expression Regulation, Neoplastic ; Hematology ; Humans ; Immunohistochemistry ; Internal Medicine ; Kaplan-Meier Estimate ; Lymph Nodes - chemistry ; Lymph Nodes - pathology ; Lymphatic Metastasis ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Oncology ; Original Article – Clinical Oncology ; Predictive Value of Tests ; Profilins - analysis ; Prognosis ; Proportional Hazards Models ; Prospective Studies ; Retrospective Studies ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stomach Neoplasms - chemistry ; Stomach Neoplasms - pathology ; Stomach Neoplasms - surgery ; Up-Regulation</subject><ispartof>Journal of cancer research and clinical oncology, 2014-10, Vol.140 (10), p.1739-1749</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-3f608a369932d86a8b06a56073432be7f72e5e6222899efc3e7ff5cfb9362d093</citedby><cites>FETCH-LOGICAL-c377t-3f608a369932d86a8b06a56073432be7f72e5e6222899efc3e7ff5cfb9362d093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00432-014-1679-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00432-014-1679-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24828259$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Li, Yan-Feng</creatorcontrib><creatorcontrib>Wang, Tie</creatorcontrib><creatorcontrib>Pang, Rui</creatorcontrib><creatorcontrib>Xue, Ying-Wei</creatorcontrib><creatorcontrib>Zhao, Shu-Peng</creatorcontrib><title>Identification of proteins associated with lymph node metastasis of gastric cancer</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><addtitle>J Cancer Res Clin Oncol</addtitle><description>Purpose
The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins.
Methods
Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellular proteins were separated by two-dimensional gel electrophoresis (2-DE), analyzed by MALDI/TOF–TOF MS, and identified by a database search. Expression of 14-3-3β and profilin-1 was then immunohistochemically verified in paraffin-embedded gastric cancer tissues from 128 patients and analyzed by multivariate logistic regression models, Kaplan–Meier curves, and Cox proportional hazard models.
Results
A total of 26 differentially expressed proteins were identified, 20 of which were overexpressed and 6 of which were underexpressed. 14-3-3β and profilin-1 were upregulated in gastric cancer tissues with and without lymph node metastasis, respectively. Expression of 14-3-3β protein was associated, but profilin-1 expression was inversely associated with gastric cancer lymph node metastasis. Multivariate analysis showed that overexpression of 14-3-3β and reduced expression of profilin-1 were independent risk factors for gastric cancer lymph node metastasis, while 14-3-3β overexpression was an independent prognostic factor for gastric cancer patients.
Conclusions
The current study identified 26 differentially expressed proteins. Further studies showed that both 14-3-3β and profilin-1 protein may be useful biomarkers for prediction of gastric cancer lymph node metastasis and that expression of 14-3-3β was a prognostic marker for gastric cancer patients.</description><subject>14-3-3 Proteins - analysis</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Cancer Research</subject><subject>Down-Regulation</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Gastrectomy</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Hematology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Internal Medicine</subject><subject>Kaplan-Meier Estimate</subject><subject>Lymph Nodes - chemistry</subject><subject>Lymph Nodes - pathology</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Oncology</subject><subject>Original Article – Clinical Oncology</subject><subject>Predictive Value of Tests</subject><subject>Profilins - analysis</subject><subject>Prognosis</subject><subject>Proportional Hazards Models</subject><subject>Prospective Studies</subject><subject>Retrospective Studies</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Stomach Neoplasms - chemistry</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - surgery</subject><subject>Up-Regulation</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMotlZ_gBuZpZvRPGaSyVKKLygIouuQydy0KfOoSYr035thqkshcJN7zznkfghdE3xHMBb3AeOC0RyTIidcyJyeoDkZO4Sx8hTNMREkLynhM3QRwhandynoOZrRoqIVLeUcvb820EdnndHRDX022GznhwiuD5kOYTBOR2iybxc3WXvodpusHxrIOog6pOPC6Finu3cmM7o34C_RmdVtgKtjXaDPp8eP5Uu-ent-XT6scsOEiDmzHFeacSkZbSquqxpzXXIsWNqgBmEFhRI4pbSSEqxhqWVLY2vJOG2wZAt0O-WmD3_tIUTVuWCgbXUPwz4oUnIieVWIUUomqfFDCB6s2nnXaX9QBKsRpZpQqoRSjSgVTZ6bY_y-7qD5c_yySwI6CUIa9WvwajvsfZ9W_if1BzeHfsc</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Ma, Yan</creator><creator>Li, Yan-Feng</creator><creator>Wang, Tie</creator><creator>Pang, Rui</creator><creator>Xue, Ying-Wei</creator><creator>Zhao, Shu-Peng</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>Identification of proteins associated with lymph node metastasis of gastric cancer</title><author>Ma, Yan ; Li, Yan-Feng ; Wang, Tie ; Pang, Rui ; Xue, Ying-Wei ; Zhao, Shu-Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-3f608a369932d86a8b06a56073432be7f72e5e6222899efc3e7ff5cfb9362d093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>14-3-3 Proteins - analysis</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Cancer Research</topic><topic>Down-Regulation</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>Gastrectomy</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Hematology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Internal Medicine</topic><topic>Kaplan-Meier Estimate</topic><topic>Lymph Nodes - chemistry</topic><topic>Lymph Nodes - pathology</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Oncology</topic><topic>Original Article – Clinical Oncology</topic><topic>Predictive Value of Tests</topic><topic>Profilins - analysis</topic><topic>Prognosis</topic><topic>Proportional Hazards Models</topic><topic>Prospective Studies</topic><topic>Retrospective Studies</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Stomach Neoplasms - chemistry</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - surgery</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Li, Yan-Feng</creatorcontrib><creatorcontrib>Wang, Tie</creatorcontrib><creatorcontrib>Pang, Rui</creatorcontrib><creatorcontrib>Xue, Ying-Wei</creatorcontrib><creatorcontrib>Zhao, Shu-Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Yan</au><au>Li, Yan-Feng</au><au>Wang, Tie</au><au>Pang, Rui</au><au>Xue, Ying-Wei</au><au>Zhao, Shu-Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of proteins associated with lymph node metastasis of gastric cancer</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><stitle>J Cancer Res Clin Oncol</stitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>140</volume><issue>10</issue><spage>1739</spage><epage>1749</epage><pages>1739-1749</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><abstract>Purpose
The aim of this study was to identify proteins associated with gastric cancer lymph node metastasis and explore the clinicopathological significance of these proteins.
Methods
Gastric cancer tissues were obtained from 24 patients with high or low lymph node metastatic potential. Total cellular proteins were separated by two-dimensional gel electrophoresis (2-DE), analyzed by MALDI/TOF–TOF MS, and identified by a database search. Expression of 14-3-3β and profilin-1 was then immunohistochemically verified in paraffin-embedded gastric cancer tissues from 128 patients and analyzed by multivariate logistic regression models, Kaplan–Meier curves, and Cox proportional hazard models.
Results
A total of 26 differentially expressed proteins were identified, 20 of which were overexpressed and 6 of which were underexpressed. 14-3-3β and profilin-1 were upregulated in gastric cancer tissues with and without lymph node metastasis, respectively. Expression of 14-3-3β protein was associated, but profilin-1 expression was inversely associated with gastric cancer lymph node metastasis. Multivariate analysis showed that overexpression of 14-3-3β and reduced expression of profilin-1 were independent risk factors for gastric cancer lymph node metastasis, while 14-3-3β overexpression was an independent prognostic factor for gastric cancer patients.
Conclusions
The current study identified 26 differentially expressed proteins. Further studies showed that both 14-3-3β and profilin-1 protein may be useful biomarkers for prediction of gastric cancer lymph node metastasis and that expression of 14-3-3β was a prognostic marker for gastric cancer patients.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>24828259</pmid><doi>10.1007/s00432-014-1679-2</doi><tpages>11</tpages></addata></record> |
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| issn | 0171-5216 1432-1335 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | 14-3-3 Proteins - analysis Adult Aged Biomarkers, Tumor - analysis Cancer Research Down-Regulation Electrophoresis, Gel, Two-Dimensional Female Gastrectomy Gene Expression Regulation, Neoplastic Hematology Humans Immunohistochemistry Internal Medicine Kaplan-Meier Estimate Lymph Nodes - chemistry Lymph Nodes - pathology Lymphatic Metastasis Male Medicine Medicine & Public Health Middle Aged Oncology Original Article – Clinical Oncology Predictive Value of Tests Profilins - analysis Prognosis Proportional Hazards Models Prospective Studies Retrospective Studies Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stomach Neoplasms - chemistry Stomach Neoplasms - pathology Stomach Neoplasms - surgery Up-Regulation |
| title | Identification of proteins associated with lymph node metastasis of gastric cancer |
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