Interaction of the unique N-terminal region of tyrosine kinase p56 super(lck) with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs
p56 super(lck), a lymphocyte-specific member of the src family of cytoplasmic protein-tyrosine kinases, is associated noncovalently with the cell surface glycoproteins CD4 and CD8, which are expressed on functionally distinct subpopulations of T cells. Using transient co-expression of p56 super(lck)...
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| Veröffentlicht in: | Cell 1990-01, Vol.60 (5), p.755-765 |
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| container_issue | 5 |
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| container_title | Cell |
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| creator | Turner, J M Brodsky, M H Irving, BA Levin, S D Perlmutter, R M Littman, DR |
| description | p56 super(lck), a lymphocyte-specific member of the src family of cytoplasmic protein-tyrosine kinases, is associated noncovalently with the cell surface glycoproteins CD4 and CD8, which are expressed on functionally distinct subpopulations of T cells. Using transient co-expression of p56 super(lck) with CD4 or CD8 alpha in COS-7 cells, we show that the unique N-terminal region of p56 super(lck) binds to the membrane-proximal 10 and 28 cytoplasmic residues of CD8 alpha and CD4, respectively. Two cysteine residues in each of the critical sequences in CD4, CD8 alpha , and p56 super(lck) are required for association. Our results suggest a novel role for cysteine-mediated interactions between unrelated proteins and provide a model for the association of other src -like cytoplasmic kinases with transmembrane proteins. |
| format | Article |
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Using transient co-expression of p56 super(lck) with CD4 or CD8 alpha in COS-7 cells, we show that the unique N-terminal region of p56 super(lck) binds to the membrane-proximal 10 and 28 cytoplasmic residues of CD8 alpha and CD4, respectively. Two cysteine residues in each of the critical sequences in CD4, CD8 alpha , and p56 super(lck) are required for association. 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Using transient co-expression of p56 super(lck) with CD4 or CD8 alpha in COS-7 cells, we show that the unique N-terminal region of p56 super(lck) binds to the membrane-proximal 10 and 28 cytoplasmic residues of CD8 alpha and CD4, respectively. Two cysteine residues in each of the critical sequences in CD4, CD8 alpha , and p56 super(lck) are required for association. Our results suggest a novel role for cysteine-mediated interactions between unrelated proteins and provide a model for the association of other src -like cytoplasmic kinases with transmembrane proteins.</abstract></addata></record> |
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| identifier | ISSN: 0092-8674 |
| ispartof | Cell, 1990-01, Vol.60 (5), p.755-765 |
| issn | 0092-8674 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15612774 |
| source | Elsevier ScienceDirect Journals |
| subjects | CD4 antigen CD8 antigen cysteine lck protein p56 |
| title | Interaction of the unique N-terminal region of tyrosine kinase p56 super(lck) with cytoplasmic domains of CD4 and CD8 is mediated by cysteine motifs |
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