Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer
Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding pr...
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| Veröffentlicht in: | Tumor biology 2014-08, Vol.35 (8), p.8143-8148 |
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| container_title | Tumor biology |
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| creator | Wang, Xue-Lei Wen, Xiao-Fei Li, Rong-Bing Liu, Bin Qiu, Guang-Ming Wen, Ji-Ling Wang, Yue-Min |
| description | Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity. |
| doi_str_mv | 10.1007/s13277-014-2085-8 |
| format | Article |
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In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-014-2085-8</identifier><identifier>PMID: 24845031</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antigens ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - physiology ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Cell Line, Tumor ; Humans ; Immunoprecipitation ; Male ; Promoter Regions, Genetic ; Prostate cancer ; Prostate-Specific Antigen - genetics ; Prostatic Neoplasms - genetics ; Protein expression ; Receptors, Androgen - physiology ; Research Article ; Ribonucleic acid ; RNA ; Transcription, Genetic</subject><ispartof>Tumor biology, 2014-08, Vol.35 (8), p.8143-8148</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2014</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ae37709732701105cae567ca88f005aa67868a221b8d2d8fb6e3c745b6ea97ae3</citedby><cites>FETCH-LOGICAL-c405t-ae37709732701105cae567ca88f005aa67868a221b8d2d8fb6e3c745b6ea97ae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-014-2085-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-014-2085-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24845031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xue-Lei</creatorcontrib><creatorcontrib>Wen, Xiao-Fei</creatorcontrib><creatorcontrib>Li, Rong-Bing</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Qiu, Guang-Ming</creatorcontrib><creatorcontrib>Wen, Ji-Ling</creatorcontrib><creatorcontrib>Wang, Yue-Min</creatorcontrib><title>Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.</description><subject>Antigens</subject><subject>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer Research</subject><subject>Cell Line, Tumor</subject><subject>Humans</subject><subject>Immunoprecipitation</subject><subject>Male</subject><subject>Promoter Regions, Genetic</subject><subject>Prostate cancer</subject><subject>Prostate-Specific Antigen - genetics</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Protein expression</subject><subject>Receptors, Androgen - physiology</subject><subject>Research Article</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Transcription, Genetic</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE9LAzEQxYMotlY_gBcJePGyOsluNtmjFP9BwYs9hzSdbbdsd9ckK_Tbm7pVRPA0A_N7b3iPkEsGtwxA3nmWcikTYFnCQYlEHZExy3iaQKrgOO7AIMm4SkfkzPsNABNFkZ-SEc9UJiBlYzKfrh0uOupw1dcmoKdhjTQ403jrqi5UbWNqamyoPqqwo21JTbN07QqbKLHYhdbRqqGda32IcmpNY9Gdk5PS1B4vDnNC5o8Pb9PnZPb69DK9nyU2AxESg6mUUMiYAhgDYQ2KXFqjVAkgjMmlypXhnC3Uki9VucgxtTITcZpCRvWE3Ay-8f97jz7obeUt1rVpsO29ZiJnLI3eRUSv_6Cbtncx3BcFCvKMQ6TYQNkYyDssdeeqrXE7zUDvO9dD5zp2rvedaxU1VwfnfrHF5Y_iu-QI8AHw8dSs0P16_a_rJ5IJi-8</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Wang, Xue-Lei</creator><creator>Wen, Xiao-Fei</creator><creator>Li, Rong-Bing</creator><creator>Liu, Bin</creator><creator>Qiu, Guang-Ming</creator><creator>Wen, Ji-Ling</creator><creator>Wang, Yue-Min</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer</title><author>Wang, Xue-Lei ; Wen, Xiao-Fei ; Li, Rong-Bing ; Liu, Bin ; Qiu, Guang-Ming ; Wen, Ji-Ling ; Wang, Yue-Min</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ae37709732701105cae567ca88f005aa67868a221b8d2d8fb6e3c745b6ea97ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antigens</topic><topic>Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - physiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cancer Research</topic><topic>Cell Line, Tumor</topic><topic>Humans</topic><topic>Immunoprecipitation</topic><topic>Male</topic><topic>Promoter Regions, Genetic</topic><topic>Prostate cancer</topic><topic>Prostate-Specific Antigen - genetics</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Protein expression</topic><topic>Receptors, Androgen - physiology</topic><topic>Research Article</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xue-Lei</creatorcontrib><creatorcontrib>Wen, Xiao-Fei</creatorcontrib><creatorcontrib>Li, Rong-Bing</creatorcontrib><creatorcontrib>Liu, Bin</creatorcontrib><creatorcontrib>Qiu, Guang-Ming</creatorcontrib><creatorcontrib>Wen, Ji-Ling</creatorcontrib><creatorcontrib>Wang, Yue-Min</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xue-Lei</au><au>Wen, Xiao-Fei</au><au>Li, Rong-Bing</au><au>Liu, Bin</au><au>Qiu, Guang-Ming</au><au>Wen, Ji-Ling</au><au>Wang, Yue-Min</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>35</volume><issue>8</issue><spage>8143</spage><epage>8148</epage><pages>8143-8148</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24845031</pmid><doi>10.1007/s13277-014-2085-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Antigens Basic Helix-Loop-Helix Leucine Zipper Transcription Factors - physiology Biomedical and Life Sciences Biomedicine Cancer Research Cell Line, Tumor Humans Immunoprecipitation Male Promoter Regions, Genetic Prostate cancer Prostate-Specific Antigen - genetics Prostatic Neoplasms - genetics Protein expression Receptors, Androgen - physiology Research Article Ribonucleic acid RNA Transcription, Genetic |
| title | Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer |
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