Identification of novel virus-specific antigens by CD4+ and CD8+ T cells from asymptomatic HSV-2 seropositive and seronegative donors
Abstract Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2pos ) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an imp...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2014-09, Vol.464, p.296-311 |
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| container_title | Virology (New York, N.Y.) |
| container_volume | 464 |
| creator | Long, Deborah Skoberne, Mojca Gierahn, Todd M Larson, Shane Price, Jessica A Clemens, Veronica Baccari, Amy E Cohane, Kenya P Garvie, Danielle Siber, George R Flechtner, Jessica B |
| description | Abstract Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2pos ) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controlling their recurrent disease symptoms. Our proteomic screening technology, ATLAS™, was used to characterize the antigenic repertoire of T cell responses in infected (HSV-2pos ) and virus-exposed seronegative (HSV-2neg ) subjects. T cell responses, determined by IFN-γ secretion, were generated to gL, UL2, UL11, UL21, ICP4, ICP0, ICP47 and UL40 with greater magnitude and/or frequency among cohorts of exposed HSV-2neg or asymptomatic HSV-2pos individuals, compared to symptomatic recurrent HSV-2pos subjects. T cell antigens recognized preferentially among individuals who are resistant to infection or who are infected and have mild or no clinical disease may provide new targets for the design of vaccines aimed at treating and/or preventing HSV-2 infection. |
| doi_str_mv | 10.1016/j.virol.2014.07.018 |
| format | Article |
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We hypothesize that infected (HSV-2pos ) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controlling their recurrent disease symptoms. Our proteomic screening technology, ATLAS™, was used to characterize the antigenic repertoire of T cell responses in infected (HSV-2pos ) and virus-exposed seronegative (HSV-2neg ) subjects. T cell responses, determined by IFN-γ secretion, were generated to gL, UL2, UL11, UL21, ICP4, ICP0, ICP47 and UL40 with greater magnitude and/or frequency among cohorts of exposed HSV-2neg or asymptomatic HSV-2pos individuals, compared to symptomatic recurrent HSV-2pos subjects. T cell antigens recognized preferentially among individuals who are resistant to infection or who are infected and have mild or no clinical disease may provide new targets for the design of vaccines aimed at treating and/or preventing HSV-2 infection.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2014.07.018</identifier><identifier>PMID: 25108380</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Antibodies, Viral - immunology ; Antigens ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; Cohort Studies ; Epitopes, T-Lymphocyte - genetics ; Epitopes, T-Lymphocyte - immunology ; Female ; Herpes Genitalis - genetics ; Herpes Genitalis - immunology ; Herpes Genitalis - virology ; Herpes simplex virus ; Herpesvirus 2, Human - genetics ; Herpesvirus 2, Human - immunology ; Humans ; Infectious Disease ; Male ; Middle Aged ; Recurrent genital herpes ; T cells ; Young Adult</subject><ispartof>Virology (New York, N.Y.), 2014-09, Vol.464, p.296-311</ispartof><rights>Elsevier Inc.</rights><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-2a51e5ee45d2c55cd61d5f03f26da5de3ce29e47d6c915672490ba28ed13788a3</citedby><cites>FETCH-LOGICAL-c459t-2a51e5ee45d2c55cd61d5f03f26da5de3ce29e47d6c915672490ba28ed13788a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2014.07.018$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25108380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Long, Deborah</creatorcontrib><creatorcontrib>Skoberne, Mojca</creatorcontrib><creatorcontrib>Gierahn, Todd M</creatorcontrib><creatorcontrib>Larson, Shane</creatorcontrib><creatorcontrib>Price, Jessica A</creatorcontrib><creatorcontrib>Clemens, Veronica</creatorcontrib><creatorcontrib>Baccari, Amy E</creatorcontrib><creatorcontrib>Cohane, Kenya P</creatorcontrib><creatorcontrib>Garvie, Danielle</creatorcontrib><creatorcontrib>Siber, George R</creatorcontrib><creatorcontrib>Flechtner, Jessica B</creatorcontrib><title>Identification of novel virus-specific antigens by CD4+ and CD8+ T cells from asymptomatic HSV-2 seropositive and seronegative donors</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2pos ) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controlling their recurrent disease symptoms. Our proteomic screening technology, ATLAS™, was used to characterize the antigenic repertoire of T cell responses in infected (HSV-2pos ) and virus-exposed seronegative (HSV-2neg ) subjects. T cell responses, determined by IFN-γ secretion, were generated to gL, UL2, UL11, UL21, ICP4, ICP0, ICP47 and UL40 with greater magnitude and/or frequency among cohorts of exposed HSV-2neg or asymptomatic HSV-2pos individuals, compared to symptomatic recurrent HSV-2pos subjects. T cell antigens recognized preferentially among individuals who are resistant to infection or who are infected and have mild or no clinical disease may provide new targets for the design of vaccines aimed at treating and/or preventing HSV-2 infection.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cohort Studies</subject><subject>Epitopes, T-Lymphocyte - genetics</subject><subject>Epitopes, T-Lymphocyte - immunology</subject><subject>Female</subject><subject>Herpes Genitalis - genetics</subject><subject>Herpes Genitalis - immunology</subject><subject>Herpes Genitalis - virology</subject><subject>Herpes simplex virus</subject><subject>Herpesvirus 2, Human - genetics</subject><subject>Herpesvirus 2, Human - immunology</subject><subject>Humans</subject><subject>Infectious Disease</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Recurrent genital herpes</subject><subject>T cells</subject><subject>Young Adult</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUk1v1DAUtBCIbgu_AAn5iFQlPNtxPg4gVUuhlSpxaOFqee2XyktiL3ay0v4A_jfObuHAhZPt8cy85_Ej5A2DkgGr32_LvYthKDmwqoSmBNY-IysGXV2AqNhzsgKoeFG3nJ-R85S2kM9NAy_JGZcMWtHCivy6tegn1zujJxc8DT31YY8Dzd5zKtIOzXJJdSY9ok90c6DrT9VlBmzetJf0gRochkT7GEaq02HcTWHMZobe3H8vOE0Ywy4kN7k9HlUL4PFRHwEbfIjpFXnR6yHh66f1gnz7fP2wvinuvn65XV_dFaaS3VRwLRlKxEpabqQ0tmZW9iB6XlstLQqDvMOqsbXpmKwbXnWw0bxFy0TTtlpckHcn310MP2dMkxpdWtrXHsOcVBYxEJLzLlPFiWpiSClir3bRjToeFAO15K-26pi_WvJX0Kicf1a9fSowb0a0fzV_As-EDycC5mfuHUaVjENv0LqIZlI2uP8U-PiP3gzO598bfuAB0zbM0ecEFVOJK1D3ywgsE8AqAMFrLn4Dw_KtWw</recordid><startdate>20140901</startdate><enddate>20140901</enddate><creator>Long, Deborah</creator><creator>Skoberne, Mojca</creator><creator>Gierahn, Todd M</creator><creator>Larson, Shane</creator><creator>Price, Jessica A</creator><creator>Clemens, Veronica</creator><creator>Baccari, Amy E</creator><creator>Cohane, Kenya P</creator><creator>Garvie, Danielle</creator><creator>Siber, George R</creator><creator>Flechtner, Jessica B</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140901</creationdate><title>Identification of novel virus-specific antigens by CD4+ and CD8+ T cells from asymptomatic HSV-2 seropositive and seronegative donors</title><author>Long, Deborah ; Skoberne, Mojca ; Gierahn, Todd M ; Larson, Shane ; Price, Jessica A ; Clemens, Veronica ; Baccari, Amy E ; Cohane, Kenya P ; Garvie, Danielle ; Siber, George R ; Flechtner, Jessica B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-2a51e5ee45d2c55cd61d5f03f26da5de3ce29e47d6c915672490ba28ed13788a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cohort Studies</topic><topic>Epitopes, T-Lymphocyte - genetics</topic><topic>Epitopes, T-Lymphocyte - immunology</topic><topic>Female</topic><topic>Herpes Genitalis - genetics</topic><topic>Herpes Genitalis - immunology</topic><topic>Herpes Genitalis - virology</topic><topic>Herpes simplex virus</topic><topic>Herpesvirus 2, Human - genetics</topic><topic>Herpesvirus 2, Human - immunology</topic><topic>Humans</topic><topic>Infectious Disease</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Recurrent genital herpes</topic><topic>T cells</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Long, Deborah</creatorcontrib><creatorcontrib>Skoberne, Mojca</creatorcontrib><creatorcontrib>Gierahn, Todd M</creatorcontrib><creatorcontrib>Larson, Shane</creatorcontrib><creatorcontrib>Price, Jessica A</creatorcontrib><creatorcontrib>Clemens, Veronica</creatorcontrib><creatorcontrib>Baccari, Amy E</creatorcontrib><creatorcontrib>Cohane, Kenya P</creatorcontrib><creatorcontrib>Garvie, Danielle</creatorcontrib><creatorcontrib>Siber, George R</creatorcontrib><creatorcontrib>Flechtner, Jessica B</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Long, Deborah</au><au>Skoberne, Mojca</au><au>Gierahn, Todd M</au><au>Larson, Shane</au><au>Price, Jessica A</au><au>Clemens, Veronica</au><au>Baccari, Amy E</au><au>Cohane, Kenya P</au><au>Garvie, Danielle</au><au>Siber, George R</au><au>Flechtner, Jessica B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of novel virus-specific antigens by CD4+ and CD8+ T cells from asymptomatic HSV-2 seropositive and seronegative donors</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2014-09-01</date><risdate>2014</risdate><volume>464</volume><spage>296</spage><epage>311</epage><pages>296-311</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Abstract Reactivation of latent herpes simplex virus 2 (HSV-2) infections can be characterized by episodic recurrent genital lesions and/or viral shedding. We hypothesize that infected (HSV-2pos ) asymptomatic individuals have acquired T cell responses to specific HSV-2 antigen(s) that may be an important factor in controlling their recurrent disease symptoms. Our proteomic screening technology, ATLAS™, was used to characterize the antigenic repertoire of T cell responses in infected (HSV-2pos ) and virus-exposed seronegative (HSV-2neg ) subjects. T cell responses, determined by IFN-γ secretion, were generated to gL, UL2, UL11, UL21, ICP4, ICP0, ICP47 and UL40 with greater magnitude and/or frequency among cohorts of exposed HSV-2neg or asymptomatic HSV-2pos individuals, compared to symptomatic recurrent HSV-2pos subjects. 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| ispartof | Virology (New York, N.Y.), 2014-09, Vol.464, p.296-311 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present) |
| subjects | Adult Aged Antibodies, Viral - immunology Antigens CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology Cohort Studies Epitopes, T-Lymphocyte - genetics Epitopes, T-Lymphocyte - immunology Female Herpes Genitalis - genetics Herpes Genitalis - immunology Herpes Genitalis - virology Herpes simplex virus Herpesvirus 2, Human - genetics Herpesvirus 2, Human - immunology Humans Infectious Disease Male Middle Aged Recurrent genital herpes T cells Young Adult |
| title | Identification of novel virus-specific antigens by CD4+ and CD8+ T cells from asymptomatic HSV-2 seropositive and seronegative donors |
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