The use of TALENs for nonhomologous end joining mutagenesis in silkworm and fruitfly

•We present an easy protocol for manual selection of TALEN targets.•We use TALEN RNA with plasmid-encoded poly(A) tails.•We show two simple TALEN activity evaluation assays based on egg microinjections.•We show mutation detection procedures based solely on molecular assays.•Our method often allows t...

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