Studies on Identifying the Catalytic Role of Glu-204 in the Active Site of Yeast Invertase

In a previous study on yeast invertase (Reddy, A., and Maley, F. (1990) J. Biol. Chem. 265, 10817-10820), we identified Asp-23 through the procedures of affinity labeling and site-directed mutagenesis as a catalytic nucleophile. In the present study we undertook to determine other residues involved...

Ausführliche Beschreibung