Radiation-induced neoplastic transformation of human prostate epithelial cells
We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray do...
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| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1996, Vol.56 (1), p.5-10 |
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| container_title | Cancer research (Chicago, Ill.) |
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| creator | KUETTEL, M. R THRAVES, P. J MIRA JUNG VARGHESE, S. P PRASAD, S. C RHIM, J. S DRITSCHILO, A |
| description | We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation. |
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R ; THRAVES, P. J ; MIRA JUNG ; VARGHESE, S. P ; PRASAD, S. C ; RHIM, J. S ; DRITSCHILO, A</creator><creatorcontrib>KUETTEL, M. R ; THRAVES, P. J ; MIRA JUNG ; VARGHESE, S. P ; PRASAD, S. C ; RHIM, J. S ; DRITSCHILO, A</creatorcontrib><description>We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 8548774</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animals ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Cell Transformation, Neoplastic - radiation effects ; Cells, Cultured ; Epithelium - metabolism ; Epithelium - pathology ; Epithelium - radiation effects ; Humans ; Karyotyping ; Keratins - biosynthesis ; Male ; Medical sciences ; Mice ; Mice, Nude ; Neoplasm Transplantation ; Physical agents ; Prostate - metabolism ; Prostate - pathology ; Prostate - radiation effects ; Prostate-Specific Antigen - biosynthesis ; Tumors</subject><ispartof>Cancer research (Chicago, Ill.), 1996, Vol.56 (1), p.5-10</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2975192$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8548774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KUETTEL, M. R</creatorcontrib><creatorcontrib>THRAVES, P. J</creatorcontrib><creatorcontrib>MIRA JUNG</creatorcontrib><creatorcontrib>VARGHESE, S. P</creatorcontrib><creatorcontrib>PRASAD, S. C</creatorcontrib><creatorcontrib>RHIM, J. S</creatorcontrib><creatorcontrib>DRITSCHILO, A</creatorcontrib><title>Radiation-induced neoplastic transformation of human prostate epithelial cells</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carcinogenesis, carcinogens and anticarcinogens</subject><subject>Cell Transformation, Neoplastic - radiation effects</subject><subject>Cells, Cultured</subject><subject>Epithelium - metabolism</subject><subject>Epithelium - pathology</subject><subject>Epithelium - radiation effects</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Keratins - biosynthesis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Neoplasm Transplantation</subject><subject>Physical agents</subject><subject>Prostate - metabolism</subject><subject>Prostate - pathology</subject><subject>Prostate - radiation effects</subject><subject>Prostate-Specific Antigen - biosynthesis</subject><subject>Tumors</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo67r6E4QcxFshSZMmPYr4BYuC6LlMmwkbST9s0oP_3qjF0zA8DzPvzBHZclWaQkupjsmWMWYKJbU4JWcxfuRWcaY2ZGOUNFrLLXl-Besh-XEo_GCXDi0dcJwCxOQ7mmYYohvn_tego6OHpYeBTvMYEySkOPl0wOAh0A5DiOfkxEGIeLHWHXm_v3u7fSz2Lw9Ptzf74iDqKhVWcDC1KZ00YAGtsaaTmhvBELV1NRqsXKZVVbe80kZIg6LWVpq25q115Y5c_83NST4XjKnpffxJADn9EhuuKqZLprJ4uYpL26Ntptn3MH816wMyv1o5xA6Cywd3Pv5reanitSi_AVDUZi8</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>KUETTEL, M. 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Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>8548774</pmid><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Carcinogenesis, carcinogens and anticarcinogens Cell Transformation, Neoplastic - radiation effects Cells, Cultured Epithelium - metabolism Epithelium - pathology Epithelium - radiation effects Humans Karyotyping Keratins - biosynthesis Male Medical sciences Mice Mice, Nude Neoplasm Transplantation Physical agents Prostate - metabolism Prostate - pathology Prostate - radiation effects Prostate-Specific Antigen - biosynthesis Tumors |
| title | Radiation-induced neoplastic transformation of human prostate epithelial cells |
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