Assessment of methods to determine minimal cellulase concentrations for efficient hydrolysis of cellulose
The enzyme loading needed to achieve substrate saturation appeared to be the most economical enzyme concentration to use for hydrolysis, based on percentage hydrolysis. Saturation was reached at 25 filter paper units per gram substrate on Solka Floc BW300, as determined by studying (a) initial adsor...
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| Veröffentlicht in: | Applied microbiology and biotechnology 1990-02, Vol.32 (5), p.614-620 |
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| container_title | Applied microbiology and biotechnology |
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| creator | Hogan, C.M Mes-Hartree, M Saddler, J.N Kushner, D.J |
| description | The enzyme loading needed to achieve substrate saturation appeared to be the most economical enzyme concentration to use for hydrolysis, based on percentage hydrolysis. Saturation was reached at 25 filter paper units per gram substrate on Solka Floc BW300, as determined by studying (a) initial adsorption of the cellulase preparation onto the substrate, (b) an actual hydrolysis or (c) a combined hydrolysis and fermentation (CHF) process. Initial adsorption of the cellulases onto the substrate can be used to determine the minimal cellulase requirements for efficient hydrolysis since enzymes initially adsorbed to the substrate have a strong role in governing the overall reaction. Trichoderma harzianum E58 produces high levels of beta-glucosidase and is able to cause high conversion of Solka Floc BW300 to glucose without the need for exogenous beta-glucosidase. End-product inhibition of the cellulase and beta-glucosidase can be more effectively reduced by employing a CHF process than by supplemental beta-glucosidase. |
| doi_str_mv | 10.1007/BF00173736 |
| format | Article |
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Saturation was reached at 25 filter paper units per gram substrate on Solka Floc BW300, as determined by studying (a) initial adsorption of the cellulase preparation onto the substrate, (b) an actual hydrolysis or (c) a combined hydrolysis and fermentation (CHF) process. Initial adsorption of the cellulases onto the substrate can be used to determine the minimal cellulase requirements for efficient hydrolysis since enzymes initially adsorbed to the substrate have a strong role in governing the overall reaction. Trichoderma harzianum E58 produces high levels of beta-glucosidase and is able to cause high conversion of Solka Floc BW300 to glucose without the need for exogenous beta-glucosidase. End-product inhibition of the cellulase and beta-glucosidase can be more effectively reduced by employing a CHF process than by supplemental beta-glucosidase.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/BF00173736</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Biological and medical sciences ; Biotechnology ; cellulase ; cellulases ; cellulose ; enzyme activity ; Enzyme engineering ; Fundamental and applied biological sciences. Psychology ; hydrolysis ; Hypocrea lixii ; Methods. Procedures. 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Saturation was reached at 25 filter paper units per gram substrate on Solka Floc BW300, as determined by studying (a) initial adsorption of the cellulase preparation onto the substrate, (b) an actual hydrolysis or (c) a combined hydrolysis and fermentation (CHF) process. Initial adsorption of the cellulases onto the substrate can be used to determine the minimal cellulase requirements for efficient hydrolysis since enzymes initially adsorbed to the substrate have a strong role in governing the overall reaction. Trichoderma harzianum E58 produces high levels of beta-glucosidase and is able to cause high conversion of Solka Floc BW300 to glucose without the need for exogenous beta-glucosidase. End-product inhibition of the cellulase and beta-glucosidase can be more effectively reduced by employing a CHF process than by supplemental beta-glucosidase.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>cellulase</subject><subject>cellulases</subject><subject>cellulose</subject><subject>enzyme activity</subject><subject>Enzyme engineering</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hydrolysis</subject><subject>Hypocrea lixii</subject><subject>Methods. Procedures. 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Psychology</topic><topic>hydrolysis</topic><topic>Hypocrea lixii</topic><topic>Methods. Procedures. Technologies</topic><topic>Others</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hogan, C.M</creatorcontrib><creatorcontrib>Mes-Hartree, M</creatorcontrib><creatorcontrib>Saddler, J.N</creatorcontrib><creatorcontrib>Kushner, D.J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hogan, C.M</au><au>Mes-Hartree, M</au><au>Saddler, J.N</au><au>Kushner, D.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of methods to determine minimal cellulase concentrations for efficient hydrolysis of cellulose</atitle><jtitle>Applied microbiology and biotechnology</jtitle><date>1990-02-01</date><risdate>1990</risdate><volume>32</volume><issue>5</issue><spage>614</spage><epage>620</epage><pages>614-620</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>The enzyme loading needed to achieve substrate saturation appeared to be the most economical enzyme concentration to use for hydrolysis, based on percentage hydrolysis. Saturation was reached at 25 filter paper units per gram substrate on Solka Floc BW300, as determined by studying (a) initial adsorption of the cellulase preparation onto the substrate, (b) an actual hydrolysis or (c) a combined hydrolysis and fermentation (CHF) process. Initial adsorption of the cellulases onto the substrate can be used to determine the minimal cellulase requirements for efficient hydrolysis since enzymes initially adsorbed to the substrate have a strong role in governing the overall reaction. Trichoderma harzianum E58 produces high levels of beta-glucosidase and is able to cause high conversion of Solka Floc BW300 to glucose without the need for exogenous beta-glucosidase. End-product inhibition of the cellulase and beta-glucosidase can be more effectively reduced by employing a CHF process than by supplemental beta-glucosidase.</abstract><cop>Berlin</cop><pub>Springer</pub><doi>10.1007/BF00173736</doi><tpages>7</tpages></addata></record> |
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| ispartof | Applied microbiology and biotechnology, 1990-02, Vol.32 (5), p.614-620 |
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| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Biological and medical sciences Biotechnology cellulase cellulases cellulose enzyme activity Enzyme engineering Fundamental and applied biological sciences. Psychology hydrolysis Hypocrea lixii Methods. Procedures. Technologies Others Various methods and equipments |
| title | Assessment of methods to determine minimal cellulase concentrations for efficient hydrolysis of cellulose |
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