Negative interference of icteric serum on a bichromatic biuret total protein assay

Background Bilirubin is stated to be a negative interferent in some biuret assays and thus could contribute to pseudohypoproteinemia in icteric samples. Objective The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when...

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Veröffentlicht in:Veterinary clinical pathology 2014-09, Vol.43 (3), p.422-427
Hauptverfasser: Gupta, Aradhana, Stockham, Steven L.
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description Background Bilirubin is stated to be a negative interferent in some biuret assays and thus could contribute to pseudohypoproteinemia in icteric samples. Objective The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when the protein concentration is measured with a bichromatic spectrophotometric biuret assay. Methods Commercially available bilirubin was dissolved in 0.1 M NaOH and mixed with sera from 2 dogs to achieve various bilirubin concentrations of up to 40 mg/dL (first set of samples) and 35 mg/dL (second set of samples, for confirmation of first set of results and to explore the interference). Biuret total protein and bilirubin concentrations were determined with a chemistry analyzer (Cobas 6000 with c501 module). Line graphs were drawn to illustrate the effects of increasing bilirubin concentrations on the total protein concentrations. Specific spectrophotometric absorbance readings were examined to identify the reason for the negative interference. Results High bilirubin concentrations created a negative interference in the Cobas biuret assay. The detectable interference occurred with a spiked bilirubin concentration of 10.7 mg/dL in one set of samples, 20.8 mg/dL in a second set. The interference was due to a greater secondary‐absorbance reading at the second measuring point in the samples spiked with bilirubin, which possibly had converted to biliverdin. Conclusion Marked hyperbilirubinemia is associated with a falsely low serum total protein concentration when measured with a bichromatic spectrophotometric biuret assay. This can result in pseudohypoproteinemia and pseudohypoglobulinemia in icteric serum.
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Objective The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when the protein concentration is measured with a bichromatic spectrophotometric biuret assay. Methods Commercially available bilirubin was dissolved in 0.1 M NaOH and mixed with sera from 2 dogs to achieve various bilirubin concentrations of up to 40 mg/dL (first set of samples) and 35 mg/dL (second set of samples, for confirmation of first set of results and to explore the interference). Biuret total protein and bilirubin concentrations were determined with a chemistry analyzer (Cobas 6000 with c501 module). Line graphs were drawn to illustrate the effects of increasing bilirubin concentrations on the total protein concentrations. Specific spectrophotometric absorbance readings were examined to identify the reason for the negative interference. Results High bilirubin concentrations created a negative interference in the Cobas biuret assay. The detectable interference occurred with a spiked bilirubin concentration of 10.7 mg/dL in one set of samples, 20.8 mg/dL in a second set. The interference was due to a greater secondary‐absorbance reading at the second measuring point in the samples spiked with bilirubin, which possibly had converted to biliverdin. Conclusion Marked hyperbilirubinemia is associated with a falsely low serum total protein concentration when measured with a bichromatic spectrophotometric biuret assay. This can result in pseudohypoproteinemia and pseudohypoglobulinemia in icteric serum.</description><identifier>ISSN: 0275-6382</identifier><identifier>EISSN: 1939-165X</identifier><identifier>DOI: 10.1111/vcp.12154</identifier><identifier>PMID: 24975994</identifier><identifier>CODEN: VCPADJ</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; Bilirubin ; Bilirubin - analysis ; Biuret - analysis ; Blood Proteins - analysis ; clinical chemistry ; Dog Diseases - blood ; Dogs ; error ; hypoproteinemia ; Hypoproteinemia - blood ; Hypoproteinemia - veterinary ; Refractometry - veterinary ; Spectrophotometry - veterinary</subject><ispartof>Veterinary clinical pathology, 2014-09, Vol.43 (3), p.422-427</ispartof><rights>2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology</rights><rights>2014 American Society for Veterinary Clinical Pathology and European Society for Veterinary Clinical Pathology.</rights><rights>Copyright © 2014 The American Society for Veterinary Clinical Pathology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4964-187c877c86291cd97645d91184ebc50289f3e3b4f8bf5b303c4261a6803fdd5f3</citedby><cites>FETCH-LOGICAL-c4964-187c877c86291cd97645d91184ebc50289f3e3b4f8bf5b303c4261a6803fdd5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fvcp.12154$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fvcp.12154$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24975994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gupta, Aradhana</creatorcontrib><creatorcontrib>Stockham, Steven L.</creatorcontrib><title>Negative interference of icteric serum on a bichromatic biuret total protein assay</title><title>Veterinary clinical pathology</title><addtitle>Vet Clin Pathol</addtitle><description>Background Bilirubin is stated to be a negative interferent in some biuret assays and thus could contribute to pseudohypoproteinemia in icteric samples. Objective The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when the protein concentration is measured with a bichromatic spectrophotometric biuret assay. Methods Commercially available bilirubin was dissolved in 0.1 M NaOH and mixed with sera from 2 dogs to achieve various bilirubin concentrations of up to 40 mg/dL (first set of samples) and 35 mg/dL (second set of samples, for confirmation of first set of results and to explore the interference). Biuret total protein and bilirubin concentrations were determined with a chemistry analyzer (Cobas 6000 with c501 module). Line graphs were drawn to illustrate the effects of increasing bilirubin concentrations on the total protein concentrations. Specific spectrophotometric absorbance readings were examined to identify the reason for the negative interference. Results High bilirubin concentrations created a negative interference in the Cobas biuret assay. The detectable interference occurred with a spiked bilirubin concentration of 10.7 mg/dL in one set of samples, 20.8 mg/dL in a second set. The interference was due to a greater secondary‐absorbance reading at the second measuring point in the samples spiked with bilirubin, which possibly had converted to biliverdin. Conclusion Marked hyperbilirubinemia is associated with a falsely low serum total protein concentration when measured with a bichromatic spectrophotometric biuret assay. This can result in pseudohypoproteinemia and pseudohypoglobulinemia in icteric serum.</description><subject>Animals</subject><subject>Bilirubin</subject><subject>Bilirubin - analysis</subject><subject>Biuret - analysis</subject><subject>Blood Proteins - analysis</subject><subject>clinical chemistry</subject><subject>Dog Diseases - blood</subject><subject>Dogs</subject><subject>error</subject><subject>hypoproteinemia</subject><subject>Hypoproteinemia - blood</subject><subject>Hypoproteinemia - veterinary</subject><subject>Refractometry - veterinary</subject><subject>Spectrophotometry - veterinary</subject><issn>0275-6382</issn><issn>1939-165X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1PFDEUhhsjkQW98A-YJt7IxUA7_Zj2UjewSGA1BoG7ptM51eJ8LO0MsP-e6gIXJpykOafN8749eRF6T8k-zXVw61b7tKSCv0IzqpkuqBRXr9GMlJUoJFPlNtpJ6ZoQJvLTG7Rdcl0JrfkM_VjCLzuGW8ChHyF6iNA7wIPHweV7cDhBnDo89NjiOrjfcegy7_I8RRjxOIy2xas4jBAykpJdv0Vb3rYJ3j32XfTz6PB8flycflt8nX8-LRzXkhdUVU5V-chSU9foSnLRaEoVh9rlPZX2DFjNvaq9qBlhjpeSWqkI800jPNtFnza--febCdJoupActK3tYZiSoUISoRgvWUY__odeD1Ps83aZEppURFCVqb0N5eKQUgRvVjF0Nq4NJeZv0CYHbf4FndkPj45T3UHzTD4lm4GDDXAXWli_7GQu5t-fLIuNIqQR7p8VNv4xsmKVMJfLhTlbnJ3ML86X5gt7AL9Flck</recordid><startdate>201409</startdate><enddate>201409</enddate><creator>Gupta, Aradhana</creator><creator>Stockham, Steven L.</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201409</creationdate><title>Negative interference of icteric serum on a bichromatic biuret total protein assay</title><author>Gupta, Aradhana ; Stockham, Steven L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4964-187c877c86291cd97645d91184ebc50289f3e3b4f8bf5b303c4261a6803fdd5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Bilirubin</topic><topic>Bilirubin - analysis</topic><topic>Biuret - analysis</topic><topic>Blood Proteins - analysis</topic><topic>clinical chemistry</topic><topic>Dog Diseases - blood</topic><topic>Dogs</topic><topic>error</topic><topic>hypoproteinemia</topic><topic>Hypoproteinemia - blood</topic><topic>Hypoproteinemia - veterinary</topic><topic>Refractometry - veterinary</topic><topic>Spectrophotometry - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gupta, Aradhana</creatorcontrib><creatorcontrib>Stockham, Steven L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gupta, Aradhana</au><au>Stockham, Steven L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Negative interference of icteric serum on a bichromatic biuret total protein assay</atitle><jtitle>Veterinary clinical pathology</jtitle><addtitle>Vet Clin Pathol</addtitle><date>2014-09</date><risdate>2014</risdate><volume>43</volume><issue>3</issue><spage>422</spage><epage>427</epage><pages>422-427</pages><issn>0275-6382</issn><eissn>1939-165X</eissn><coden>VCPADJ</coden><abstract>Background Bilirubin is stated to be a negative interferent in some biuret assays and thus could contribute to pseudohypoproteinemia in icteric samples. Objective The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when the protein concentration is measured with a bichromatic spectrophotometric biuret assay. Methods Commercially available bilirubin was dissolved in 0.1 M NaOH and mixed with sera from 2 dogs to achieve various bilirubin concentrations of up to 40 mg/dL (first set of samples) and 35 mg/dL (second set of samples, for confirmation of first set of results and to explore the interference). Biuret total protein and bilirubin concentrations were determined with a chemistry analyzer (Cobas 6000 with c501 module). Line graphs were drawn to illustrate the effects of increasing bilirubin concentrations on the total protein concentrations. Specific spectrophotometric absorbance readings were examined to identify the reason for the negative interference. Results High bilirubin concentrations created a negative interference in the Cobas biuret assay. The detectable interference occurred with a spiked bilirubin concentration of 10.7 mg/dL in one set of samples, 20.8 mg/dL in a second set. The interference was due to a greater secondary‐absorbance reading at the second measuring point in the samples spiked with bilirubin, which possibly had converted to biliverdin. Conclusion Marked hyperbilirubinemia is associated with a falsely low serum total protein concentration when measured with a bichromatic spectrophotometric biuret assay. This can result in pseudohypoproteinemia and pseudohypoglobulinemia in icteric serum.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24975994</pmid><doi>10.1111/vcp.12154</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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source Wiley Online Library - AutoHoldings Journals; MEDLINE
subjects Animals
Bilirubin
Bilirubin - analysis
Biuret - analysis
Blood Proteins - analysis
clinical chemistry
Dog Diseases - blood
Dogs
error
hypoproteinemia
Hypoproteinemia - blood
Hypoproteinemia - veterinary
Refractometry - veterinary
Spectrophotometry - veterinary
title Negative interference of icteric serum on a bichromatic biuret total protein assay
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