Do spawn storage conditions influence the colonization capacity of a wheat-straw-based substrate by Agaricus subrufescens?

Storage conditions of the spawn of edible fungi are of major importance to facilitate the production of mushrooms. Here, standard storage conditions at 10°C or 15°C were used and the potential of colonization of standard European compost by the tropical species Agaricus subrufescens was assessed dur...

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Veröffentlicht in:Comptes Rendus Biologies 2014-07, Vol.337 (7-8), p.443-450
Hauptverfasser: Farnet, Anne-Marie, Qasemian, Leila, Peter-Valence, Frédérique, Ruaudel, Florence, Savoie, Jean-Michel, Roussos, Sevastianos, Gaime-Perraud, Isabelle, Ziarelli, Fabio, Ferré, Élisée
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container_issue 7-8
container_start_page 443
container_title Comptes Rendus Biologies
container_volume 337
creator Farnet, Anne-Marie
Qasemian, Leila
Peter-Valence, Frédérique
Ruaudel, Florence
Savoie, Jean-Michel
Roussos, Sevastianos
Gaime-Perraud, Isabelle
Ziarelli, Fabio
Ferré, Élisée
description Storage conditions of the spawn of edible fungi are of major importance to facilitate the production of mushrooms. Here, standard storage conditions at 10°C or 15°C were used and the potential of colonization of standard European compost by the tropical species Agaricus subrufescens was assessed during the spawn running phase. Two lignocellulolytic activities, laccase and CMC-cellulase, were enhanced after storage compared to control as well as substrate transformation, as described by the aromaticity ratio and a humification ratio calculated from NMR data. This result indicates that mycelium growth probably occurred during storage at 10 or 15°C, leading to a larger amount of biomass in the inoculum. Moreover, the microbial functional diversity of the substrate was favored, showing that the electivity of the substrate was maintained. Thus, these findings indicate that recommendations for the mushroom producers can be established for A. subrufescens cultivation under European standard conditions. Les conditions de stockage du blanc des champignons comestibles sont de première importance pour faciliter la production de champignons. Ici, des conditions standard de stockage à 10 et 15°C ont été utilisées et le potentiel de colonisation d’un compost européen classique par l’espèce tropicale Agaricus subrufescens a été estimé durant la phase de colonisation du blanc. Les activités lignocellulolytiques laccase et CMC-cellulase ont été activées après stockage en comparaison avec le contrôle ainsi que la transformation du substrat, comme décrit par les ratios d’aromaticité et d’humification calculés à partir des données de RMN. Ce résultat indique qu’une croissance mycélienne a probablement eu lieu durant la période de stockage à 10 ou 15°C, conduisant à une plus grande quantité de biomasse dans l’inoculum. De plus, la diversité fonctionnelle microbienne du substrat a été favorisée, montrant que l’électivité du substrat était maintenue. Ainsi, ces recherches indiquent que des recommandations auprès des producteurs de champignons peuvent être formulées pour la culture d’Agaricus subrufescens dans les conditions standard utilisées en Europe.
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Here, standard storage conditions at 10°C or 15°C were used and the potential of colonization of standard European compost by the tropical species Agaricus subrufescens was assessed during the spawn running phase. Two lignocellulolytic activities, laccase and CMC-cellulase, were enhanced after storage compared to control as well as substrate transformation, as described by the aromaticity ratio and a humification ratio calculated from NMR data. This result indicates that mycelium growth probably occurred during storage at 10 or 15°C, leading to a larger amount of biomass in the inoculum. Moreover, the microbial functional diversity of the substrate was favored, showing that the electivity of the substrate was maintained. Thus, these findings indicate that recommendations for the mushroom producers can be established for A. subrufescens cultivation under European standard conditions. Les conditions de stockage du blanc des champignons comestibles sont de première importance pour faciliter la production de champignons. Ici, des conditions standard de stockage à 10 et 15°C ont été utilisées et le potentiel de colonisation d’un compost européen classique par l’espèce tropicale Agaricus subrufescens a été estimé durant la phase de colonisation du blanc. Les activités lignocellulolytiques laccase et CMC-cellulase ont été activées après stockage en comparaison avec le contrôle ainsi que la transformation du substrat, comme décrit par les ratios d’aromaticité et d’humification calculés à partir des données de RMN. Ce résultat indique qu’une croissance mycélienne a probablement eu lieu durant la période de stockage à 10 ou 15°C, conduisant à une plus grande quantité de biomasse dans l’inoculum. De plus, la diversité fonctionnelle microbienne du substrat a été favorisée, montrant que l’électivité du substrat était maintenue. 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subjects Agaricales - growth & development
Agaricus
Agaricus - growth & development
Agriculture
Almond mushroom
Bacteria - growth & development
Biotechnology
Champignon amande
Culture de champignons
Environmental Sciences
Enzymes lignocellulolytiques
Fermentation
Life Sciences
Lignocellulolytic enzymes
Magnetic Resonance Spectroscopy
Metabolism
Microbiology and Parasitology
Mushroom cultivation
Mycelium - growth & development
Mycology
RMN du solide du 13C
Soil
Solid-state NMR of 13C
Temperature
Triticum - microbiology
title Do spawn storage conditions influence the colonization capacity of a wheat-straw-based substrate by Agaricus subrufescens?
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