Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran
CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine ph...
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description | CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran. |
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The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran.</description><identifier>ISSN: 1234-1983</identifier><identifier>EISSN: 2190-3883</identifier><identifier>DOI: 10.1007/s13353-014-0209-x</identifier><identifier>PMID: 24771298</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>3' Untranslated Regions - genetics ; Amino Acid Motifs - genetics ; Animal Genetics and Genomics ; Antigens, Bacterial - genetics ; Bacterial genetics ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; DNA ; DNA, Bacterial - genetics ; Genes ; Genetic research ; Genetic Variation - genetics ; Helicobacter Infections - genetics ; Helicobacter Infections - virology ; Helicobacter pylori ; Helicobacter pylori - genetics ; Helicobacter pylori - isolation & purification ; Helicobacter pylori - pathogenicity ; Human Genetics ; Humans ; Life Sciences ; Microbial Genetics and Genomics ; Microbial Genetics • Original Paper ; monitoring ; Phenols ; Phosphorylation ; Plant Genetics and Genomics ; Polymerase Chain Reaction ; Time Factors ; Tyrosine ; Tyrosine - chemistry ; Tyrosine - genetics ; virulence</subject><ispartof>Journal of applied genetics, 2014-08, Vol.55 (3), p.417-422</ispartof><rights>Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-46eb6178d15ca5972e064fb945046a92966b16dd86a6e1e81da2fee6efe8f0f83</citedby><cites>FETCH-LOGICAL-c501t-46eb6178d15ca5972e064fb945046a92966b16dd86a6e1e81da2fee6efe8f0f83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13353-014-0209-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13353-014-0209-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24771298$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kargar, Mohammad</creatorcontrib><creatorcontrib>Ghorbani-Dalini, Sadegh</creatorcontrib><creatorcontrib>Doosti, Abbas</creatorcontrib><creatorcontrib>Najafi, Akram</creatorcontrib><title>Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran</title><title>Journal of applied genetics</title><addtitle>J Appl Genetics</addtitle><addtitle>J Appl Genet</addtitle><description>CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran.</description><subject>3' Untranslated Regions - genetics</subject><subject>Amino Acid Motifs - genetics</subject><subject>Animal Genetics and Genomics</subject><subject>Antigens, Bacterial - genetics</subject><subject>Bacterial genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetic Variation - genetics</subject><subject>Helicobacter Infections - genetics</subject><subject>Helicobacter Infections - virology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - genetics</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Helicobacter pylori - pathogenicity</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbial Genetics • Original Paper</subject><subject>monitoring</subject><subject>Phenols</subject><subject>Phosphorylation</subject><subject>Plant Genetics and Genomics</subject><subject>Polymerase Chain Reaction</subject><subject>Time Factors</subject><subject>Tyrosine</subject><subject>Tyrosine - chemistry</subject><subject>Tyrosine - genetics</subject><subject>virulence</subject><issn>1234-1983</issn><issn>2190-3883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVFr1TAYhoso7jj9Ad5owBtvOvMlbZJeHoZzg4EXuuuQtl96MtrkmPSMFfzxpnQKgkgIgfA8L8n3FsVboBdAqfyUgPOalxSqkjLalI_Pih2DhpZcKf682AHjVQmN4mfFq5TuKeWqkuxlccYqKYE1alf8vHIPWC5oIpmCd3OIzg8kWNIFn1yP0bQjku5g_ICJOE_mJYbkPJLjIaS84zKa2QWf9dnZtKrzAck1jq4LrelmjOS4jDmXdGbYkwGzm3NuovGvixfWjAnfPJ3nxd3V5--X1-Xt1y83l_vbsqspzGUlsBUgVQ91Z-pGMqSism1T1bQSpmGNEC2IvlfCCARU0BtmEQVaVJZaxc-Lj1vuMYYfJ0yznlzqcByNx3BKGmpBIc8EREY_bOhgRtTO2zBH06243ksqZSOlkJm6-AeVV49T_rZH6_L9XwJsQpenlyJafYxuMnHRQPXapd661LlLvXapH7Pz7unVp3bC_o_xu7wMsA1Ix7U1jPo-nKLPk_xv6vtNsiZoM0SX9N03lgFKgdcNSP4Ly6O0Ag</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Kargar, Mohammad</creator><creator>Ghorbani-Dalini, Sadegh</creator><creator>Doosti, Abbas</creator><creator>Najafi, Akram</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20140801</creationdate><title>Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran</title><author>Kargar, Mohammad ; Ghorbani-Dalini, Sadegh ; Doosti, Abbas ; Najafi, Akram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-46eb6178d15ca5972e064fb945046a92966b16dd86a6e1e81da2fee6efe8f0f83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3' Untranslated Regions - genetics</topic><topic>Amino Acid Motifs - genetics</topic><topic>Animal Genetics and Genomics</topic><topic>Antigens, Bacterial - genetics</topic><topic>Bacterial genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genetic Variation - genetics</topic><topic>Helicobacter Infections - genetics</topic><topic>Helicobacter Infections - virology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - genetics</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Helicobacter pylori - pathogenicity</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbial Genetics • Original Paper</topic><topic>monitoring</topic><topic>Phenols</topic><topic>Phosphorylation</topic><topic>Plant Genetics and Genomics</topic><topic>Polymerase Chain Reaction</topic><topic>Time Factors</topic><topic>Tyrosine</topic><topic>Tyrosine - chemistry</topic><topic>Tyrosine - genetics</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kargar, Mohammad</creatorcontrib><creatorcontrib>Ghorbani-Dalini, Sadegh</creatorcontrib><creatorcontrib>Doosti, Abbas</creatorcontrib><creatorcontrib>Najafi, Akram</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kargar, Mohammad</au><au>Ghorbani-Dalini, Sadegh</au><au>Doosti, Abbas</au><au>Najafi, Akram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran</atitle><jtitle>Journal of applied genetics</jtitle><stitle>J Appl Genetics</stitle><addtitle>J Appl Genet</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>55</volume><issue>3</issue><spage>417</spage><epage>422</epage><pages>417-422</pages><issn>1234-1983</issn><eissn>2190-3883</eissn><abstract>CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24771298</pmid><doi>10.1007/s13353-014-0209-x</doi><tpages>6</tpages></addata></record> |
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subjects | 3' Untranslated Regions - genetics Amino Acid Motifs - genetics Animal Genetics and Genomics Antigens, Bacterial - genetics Bacterial genetics Bacterial Proteins - genetics Biomedical and Life Sciences DNA DNA, Bacterial - genetics Genes Genetic research Genetic Variation - genetics Helicobacter Infections - genetics Helicobacter Infections - virology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Helicobacter pylori - pathogenicity Human Genetics Humans Life Sciences Microbial Genetics and Genomics Microbial Genetics • Original Paper monitoring Phenols Phosphorylation Plant Genetics and Genomics Polymerase Chain Reaction Time Factors Tyrosine Tyrosine - chemistry Tyrosine - genetics virulence |
title | Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran |
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