Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran

CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine ph...

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Veröffentlicht in:Journal of applied genetics 2014-08, Vol.55 (3), p.417-422
Hauptverfasser: Kargar, Mohammad, Ghorbani-Dalini, Sadegh, Doosti, Abbas, Najafi, Akram
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container_issue 3
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creator Kargar, Mohammad
Ghorbani-Dalini, Sadegh
Doosti, Abbas
Najafi, Akram
description CagA is a major virulence factor of Helicobacter pylori involved in host cell modulation. The C-terminal part of CagA containing the EPIYA motifs is highly variable and is important for the biological activity of the protein. The aim of this study was consideration of the changes in cagA tyrosine phosphorylation motifs (TPMs) of H. pylori. A set of 302 H. pylori DNA samples from the Iranian population from 2006 to 2011 was selected for the proposed study. The cagA gene and its TPMs were assessed by using polymerase chain reaction (PCR) and specific primers. The prevalence of the cagA gene in our study ranged from 91.43 % to 97.06 % (with an average of 95.03 %). Out of the cagA-positive samples, the prevalence of TPMs A and B increased from 12.5 % and 23.44 % to 71.2 % and 63.63 %, respectively. Also, the prevalence of samples infected with Western and East Asian types of H. pylori ranged from 64.06 % to 5.73 % for the Western type and 17.19 % to 51.59 % for the East Asian type. Overall, our results showed a high prevalence of the cagA gene. Also, it seems that cagA TPMs of H. pylori is undergoing a change from the Western type to the East Asian type in Iran.
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subjects 3' Untranslated Regions - genetics
Amino Acid Motifs - genetics
Animal Genetics and Genomics
Antigens, Bacterial - genetics
Bacterial genetics
Bacterial Proteins - genetics
Biomedical and Life Sciences
DNA
DNA, Bacterial - genetics
Genes
Genetic research
Genetic Variation - genetics
Helicobacter Infections - genetics
Helicobacter Infections - virology
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Helicobacter pylori - pathogenicity
Human Genetics
Humans
Life Sciences
Microbial Genetics and Genomics
Microbial Genetics • Original Paper
monitoring
Phenols
Phosphorylation
Plant Genetics and Genomics
Polymerase Chain Reaction
Time Factors
Tyrosine
Tyrosine - chemistry
Tyrosine - genetics
virulence
title Five-year monitoring of considerable changes in tyrosine phosphorylation motifs of the Helicobacter pylori cagA gene in Iran
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