Development and validation of a mtDNA multiplex PCR for identification and discrimination of Calicophoron daubneyi and Fasciola hepatica in the Galba truncatula snail

Paramphistomosis and Fasciolosis caused by Calicophoron daubneyi and Fasciola hepatica, respectively, are frequent and important trematodoses in ruminant livestock worldwide. Both parasites use the same snail, Galba truncatula, as intermediate host. The aim of this study was to develop and validate...

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Veröffentlicht in:	Veterinary parasitology 2013-07, Vol.195 (1-2), p.57-64
Hauptverfasser:	Martínez-Ibeas, A.M., González-Warleta, M., Martínez-Valladares, M., Castro-Hermida, J.A., González-Lanza, C., Miñambres, B., Ferreras, C., Mezo, M., Manga-González, M.Y.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Calicophoron daubneyi Cattle Cattle Diseases - diagnosis Cattle Diseases - parasitology Cotylophoron Dicrocoelium dendriticum Digenea DNA Primers - genetics DNA, Helminth - chemistry DNA, Helminth - genetics DNA, Mitochondrial - chemistry DNA, Mitochondrial - genetics DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Early Diagnosis Fasciola hepatica Fasciola hepatica - genetics Fasciola hepatica - isolation & purification Fascioliasis - diagnosis Fascioliasis - parasitology Fascioliasis - veterinary Galba truncatula Galba truncatula snail Hepatica Lymnaea - parasitology Marine Mitochondrial DNA (mtDNA) Molecular Sequence Data Mollusca Multiplex PCR identification Multiplex Polymerase Chain Reaction - veterinary Notocotylidae Paramphistomatidae - genetics Paramphistomatidae - isolation & purification Plagiorchiidae Ruminantia Sensitivity and Specificity Sequence Analysis, DNA - veterinary Sheep Sheep Diseases - diagnosis Sheep Diseases - parasitology Species Specificity Trematode Infections - diagnosis Trematode Infections - parasitology Trematode Infections - veterinary
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