Efficient simultaneous excision of multiple selectable marker cassettes using I‐SceI‐induced double‐strand DNA breaks in Saccharomyces cerevisiae

Large strain construction programs and functional analysis studies are becoming commonplace in Saccharomyces cerevisiae and involve construction of strains that carry multiple selectable marker genes. Extensive strain engineering is, however, severely hampered by the limited number of recyclable mar...

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Veröffentlicht in:	FEMS yeast research 2014-08, Vol.14 (5), p.741-754
Hauptverfasser:	Solis‐Escalante, Daniel, Kuijpers, Niels G.A, Linden, Franka H, Pronk, Jack T, Daran, Jean‐Marc, Daran‐Lapujade, Pascale
Format:	Artikel
Sprache:	eng
Schlagworte:	auxotrophic marker Auxotrophy budding yeast Deoxyribonucleases, Type II Site-Specific - metabolism DNA Breaks, Double-Stranded DNA damage dominant marker double‐strand DNA break engineering Gene Deletion genetic markers Genetics, Microbial - methods genome Genomes Homologous Recombination I‐SceI marker excision Metabolic Engineering - methods Mutagenesis, Insertional - methods Plasmids polymerase chain reaction Recombinase Recycling Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - metabolism Selection, Genetic Yeast yeasts
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