Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis
The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Ps...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2013-10, Vol.116 (4), p.485-492 |
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| creator | Możejko, Justyna Ciesielski, Slawomir |
| description | The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Pseudomonas sp. Gl01 was grown for 48 h in a biofermentor containing 15 g/l of SWPO. The results clearly indicate that lower carbon source supplementation decreased mcl-PHAs production. Furthermore, the obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis. Additionally, in the present study the mcl-PHAs biosynthesis at the molecular level was also investigated. Using the RT real-time PCR technique, the expression of PHA synthase genes (phaC1 and phaC2) and PHA depolymerase gene (phaZ) was analyzed. The data suggest that the phaZ gene could be transcribed together with the phaC1 or phaC2 gene, which means that PHA synthesis and degradation followed simultaneously. Depending on the oily substrate concentration a wide range of repeat-unit components were observed. The purified polymers consisted of monomers ranging from C6 to C16. Moreover, a differential scanning calorimetric and gel permeation chromatography analysis confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties. |
| doi_str_mv | 10.1016/j.jbiosc.2013.04.014 |
| format | Article |
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Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Pseudomonas sp. Gl01 was grown for 48 h in a biofermentor containing 15 g/l of SWPO. The results clearly indicate that lower carbon source supplementation decreased mcl-PHAs production. Furthermore, the obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis. Additionally, in the present study the mcl-PHAs biosynthesis at the molecular level was also investigated. Using the RT real-time PCR technique, the expression of PHA synthase genes (phaC1 and phaC2) and PHA depolymerase gene (phaZ) was analyzed. The data suggest that the phaZ gene could be transcribed together with the phaC1 or phaC2 gene, which means that PHA synthesis and degradation followed simultaneously. Depending on the oily substrate concentration a wide range of repeat-unit components were observed. The purified polymers consisted of monomers ranging from C6 to C16. Moreover, a differential scanning calorimetric and gel permeation chromatography analysis confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties.</description><identifier>ISSN: 1389-1723</identifier><identifier>EISSN: 1347-4421</identifier><identifier>DOI: 10.1016/j.jbiosc.2013.04.014</identifier><identifier>PMID: 23706994</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acyltransferases - genetics ; Batch Cell Culture Techniques ; Biological and medical sciences ; Biopolymers ; biosynthesis ; Biotechnology ; calorimetry ; carbon ; Carbon - metabolism ; Carbon - pharmacology ; Carboxylic Ester Hydrolases - genetics ; Conservation of Natural Resources - methods ; Fundamental and applied biological sciences. Psychology ; gel chromatography ; Gene Expression Regulation, Bacterial ; genes ; Mcl-polyhydroxyalkanoates ; Molecular Weight ; nitrogen ; Palm Oil ; palm oils ; physicochemical properties ; Plant Oils - chemistry ; Plant Oils - metabolism ; Polyhydroxyalkanoates - biosynthesis ; Polyhydroxyalkanoates - chemistry ; Pseudomonas ; Pseudomonas - drug effects ; Pseudomonas - genetics ; Pseudomonas - growth & development ; Pseudomonas - metabolism ; quantitative polymerase chain reaction ; Real-time PCR ; Real-Time Polymerase Chain Reaction ; Recycling ; renewable resources ; Temperature ; Thermal properties ; Transcription, Genetic ; Waste plant oil</subject><ispartof>Journal of bioscience and bioengineering, 2013-10, Vol.116 (4), p.485-492</ispartof><rights>2013 The Society for Biotechnology, Japan</rights><rights>2014 INIST-CNRS</rights><rights>Copyright © 2013 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c542t-7ca288b8901a0b760e60b58c363d4d405b14a7a51920067cd4106e99b64326223</citedby><cites>FETCH-LOGICAL-c542t-7ca288b8901a0b760e60b58c363d4d405b14a7a51920067cd4106e99b64326223</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jbiosc.2013.04.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27864133$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23706994$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Możejko, Justyna</creatorcontrib><creatorcontrib>Ciesielski, Slawomir</creatorcontrib><title>Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis</title><title>Journal of bioscience and bioengineering</title><addtitle>J Biosci Bioeng</addtitle><description>The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Pseudomonas sp. Gl01 was grown for 48 h in a biofermentor containing 15 g/l of SWPO. The results clearly indicate that lower carbon source supplementation decreased mcl-PHAs production. Furthermore, the obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis. Additionally, in the present study the mcl-PHAs biosynthesis at the molecular level was also investigated. Using the RT real-time PCR technique, the expression of PHA synthase genes (phaC1 and phaC2) and PHA depolymerase gene (phaZ) was analyzed. The data suggest that the phaZ gene could be transcribed together with the phaC1 or phaC2 gene, which means that PHA synthesis and degradation followed simultaneously. Depending on the oily substrate concentration a wide range of repeat-unit components were observed. The purified polymers consisted of monomers ranging from C6 to C16. Moreover, a differential scanning calorimetric and gel permeation chromatography analysis confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties.</description><subject>Acyltransferases - genetics</subject><subject>Batch Cell Culture Techniques</subject><subject>Biological and medical sciences</subject><subject>Biopolymers</subject><subject>biosynthesis</subject><subject>Biotechnology</subject><subject>calorimetry</subject><subject>carbon</subject><subject>Carbon - metabolism</subject><subject>Carbon - pharmacology</subject><subject>Carboxylic Ester Hydrolases - genetics</subject><subject>Conservation of Natural Resources - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gel chromatography</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>genes</subject><subject>Mcl-polyhydroxyalkanoates</subject><subject>Molecular Weight</subject><subject>nitrogen</subject><subject>Palm Oil</subject><subject>palm oils</subject><subject>physicochemical properties</subject><subject>Plant Oils - chemistry</subject><subject>Plant Oils - metabolism</subject><subject>Polyhydroxyalkanoates - biosynthesis</subject><subject>Polyhydroxyalkanoates - chemistry</subject><subject>Pseudomonas</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - growth & development</subject><subject>Pseudomonas - metabolism</subject><subject>quantitative polymerase chain reaction</subject><subject>Real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Recycling</subject><subject>renewable resources</subject><subject>Temperature</subject><subject>Thermal properties</subject><subject>Transcription, Genetic</subject><subject>Waste plant oil</subject><issn>1389-1723</issn><issn>1347-4421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1TAQBuAIgWgpvAGCbJDYJIwvseMNEqq4SZVYlK6tiTOhPiRxsHNa8vYkygF2sPIsvpmx5s-y5wxKBky9OZSHxofkSg5MlCBLYPJBds6E1IWUnD3c6toUTHNxlj1J6QDANGj2ODvjQoMyRp5n7TVOYfSdpza_xzRTPmE_5MH3OaYcxxznOaKb_R3lkUa6x6bfqhSO0VHehZgPri-m0C-3SxvDzwX77zgGXCelZZxvKfn0NHvUYZ_o2em9yG4-vP96-am4-vLx8-W7q8JVks-FdsjruqkNMIRGKyAFTVU7oUQrWwlVwyRqrJjhAEq7VjJQZEyjpOCKc3GRvd7nTjH8OFKa7eCTo77HkcIxWVYpYKyujfw_ldwoWQm2TZU7dTGkFKmzU_QDxsUysFsU9mD3KOwWhQVp1yjWthenDcdmoPZP0-_br-DVCWBy2HcRR-fTX6drJZkQq3u5uw6DxW9xNTfX6yYFAJzXRq_i7S5oPe6dp2iT8zQ6an0kN9s2-H__9RcjJrHc</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Możejko, Justyna</creator><creator>Ciesielski, Slawomir</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20131001</creationdate><title>Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis</title><author>Możejko, Justyna ; Ciesielski, Slawomir</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c542t-7ca288b8901a0b760e60b58c363d4d405b14a7a51920067cd4106e99b64326223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Acyltransferases - genetics</topic><topic>Batch Cell Culture Techniques</topic><topic>Biological and medical sciences</topic><topic>Biopolymers</topic><topic>biosynthesis</topic><topic>Biotechnology</topic><topic>calorimetry</topic><topic>carbon</topic><topic>Carbon - metabolism</topic><topic>Carbon - pharmacology</topic><topic>Carboxylic Ester Hydrolases - genetics</topic><topic>Conservation of Natural Resources - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gel chromatography</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>genes</topic><topic>Mcl-polyhydroxyalkanoates</topic><topic>Molecular Weight</topic><topic>nitrogen</topic><topic>Palm Oil</topic><topic>palm oils</topic><topic>physicochemical properties</topic><topic>Plant Oils - chemistry</topic><topic>Plant Oils - metabolism</topic><topic>Polyhydroxyalkanoates - biosynthesis</topic><topic>Polyhydroxyalkanoates - chemistry</topic><topic>Pseudomonas</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - growth & development</topic><topic>Pseudomonas - metabolism</topic><topic>quantitative polymerase chain reaction</topic><topic>Real-time PCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Recycling</topic><topic>renewable resources</topic><topic>Temperature</topic><topic>Thermal properties</topic><topic>Transcription, Genetic</topic><topic>Waste plant oil</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Możejko, Justyna</creatorcontrib><creatorcontrib>Ciesielski, Slawomir</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Możejko, Justyna</au><au>Ciesielski, Slawomir</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2013-10-01</date><risdate>2013</risdate><volume>116</volume><issue>4</issue><spage>485</spage><epage>492</epage><pages>485-492</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>The synthesis of mcl-polyhydroxyalkanoates (mcl-PHAs) by Pseudomonas sp. Gl01 using saponified waste palm oil (SWPO) as the sole carbon source was investigated. It was shown that the analyzed strain accumulated biopolymers during the growth phase. Up to 43% of mcl-PHAs at 17 h were produced, when Pseudomonas sp. Gl01 was grown for 48 h in a biofermentor containing 15 g/l of SWPO. The results clearly indicate that lower carbon source supplementation decreased mcl-PHAs production. Furthermore, the obtained results confirmed that nitrogen limitation is unnecessary for the stimulation of biopolymer synthesis. Additionally, in the present study the mcl-PHAs biosynthesis at the molecular level was also investigated. Using the RT real-time PCR technique, the expression of PHA synthase genes (phaC1 and phaC2) and PHA depolymerase gene (phaZ) was analyzed. The data suggest that the phaZ gene could be transcribed together with the phaC1 or phaC2 gene, which means that PHA synthesis and degradation followed simultaneously. Depending on the oily substrate concentration a wide range of repeat-unit components were observed. The purified polymers consisted of monomers ranging from C6 to C16. Moreover, a differential scanning calorimetric and gel permeation chromatography analysis confirmed that the extracted mcl-PHAs are elastomers with useful physical and chemical properties.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>23706994</pmid><doi>10.1016/j.jbiosc.2013.04.014</doi><tpages>8</tpages></addata></record> |
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| subjects | Acyltransferases - genetics Batch Cell Culture Techniques Biological and medical sciences Biopolymers biosynthesis Biotechnology calorimetry carbon Carbon - metabolism Carbon - pharmacology Carboxylic Ester Hydrolases - genetics Conservation of Natural Resources - methods Fundamental and applied biological sciences. Psychology gel chromatography Gene Expression Regulation, Bacterial genes Mcl-polyhydroxyalkanoates Molecular Weight nitrogen Palm Oil palm oils physicochemical properties Plant Oils - chemistry Plant Oils - metabolism Polyhydroxyalkanoates - biosynthesis Polyhydroxyalkanoates - chemistry Pseudomonas Pseudomonas - drug effects Pseudomonas - genetics Pseudomonas - growth & development Pseudomonas - metabolism quantitative polymerase chain reaction Real-time PCR Real-Time Polymerase Chain Reaction Recycling renewable resources Temperature Thermal properties Transcription, Genetic Waste plant oil |
| title | Saponified waste palm oil as an attractive renewable resource for mcl-polyhydroxyalkanoate synthesis |
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