A Dynamic Interface between Vacuoles and Mitochondria in Yeast

Cellular life depends on continuous transport of lipids and small molecules between mitochondria and the endomembrane system. Recently, endoplasmic reticulum-mitochondrial encounter structure (ERMES) was identified as an important yet nonessential contact for such transport. Using a high-content screen in yeast, we found a contact site, marked by Vam6/Vps39, between vacuoles (the yeast lysosomal compartment) and mitochondria, named vCLAMP (vacuole and mitochondria patch). vCLAMP is enriched with ion and amino-acid transporters and has a role in lipid relay between the endomembrane system and mitochondria. Critically, we show that mitochondria are dependent on having one of two contact sites, ERMES or vCLAMP. The absence of one causes expansion of the other, and elimination of both is lethal. Identification of vCLAMP adds to our ability to understand the complexity of interorganellar crosstalk. [Display omitted] •A membrane contact site exists between mitochondria and vacuoles (yeast lysosomes)•Mitochondrial contact sites with the ER and with vacuoles are coregulated•Mitochondria require at least one contact site with the endomembrane system•Loss of both mitochondrial contact sites impairs phospholipid transport Organelle communication is instrumental in coordinating cellular function and can be achieved by creation of membrane contact sites (MCSs). Elbaz-Alon et al. uncover MCSs in yeast between vacuoles (the yeast lysosomes) and mitochondria. These vacuole and mitochondria patches (vCLAMPs) are coregulated with ER/mitochondria MCSs for efficient transfer of phospholipids.