EvaGreen Real-time PCR protocol for specific ‘Candidatus Phytoplasma mali’ detection and quantification in insects

In this paper the validation and implementation of a Real-time PCR protocol based on ribosomal protein genes has been carried out for sensitive and specific quantification of ‘Candidatus (Ca.) Phytoplasma mali’ (apple proliferation phytoplasma, APP) in insects. The method combines the use of EvaGree...

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Veröffentlicht in:	Molecular and cellular probes 2013-06, Vol.27 (3-4), p.129-136
Hauptverfasser:	Monti, Monia, Martini, Marta, Tedeschi, Rosemarie
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cacopsylla melanoneura Candidatus Phytoplasma mali Coloring Agents - chemistry DNA Primers - chemistry DNA, Bacterial - analysis DNA, Ribosomal - chemistry EvaGreen® dye Fluorescent Dyes - chemistry Insecta - genetics Insecta - microbiology Insects Malus Phytoplasma - classification Phytoplasma - genetics Real-time PCR Real-Time Polymerase Chain Reaction - methods Reproducibility of Results Ribosomal protein gene
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