Domain Closure in Adenylate Kinase

The method of time-resolved dynamic nonradiative excitation energy transfer (ET) was used to analyze the proposed domain closure in adenylate kinase (AK). A highly active mutant of Escherichia coli AK, (C77S, V169W, A55C)-AK, was prepared, in which the solvent-accessible residues valine 169 and alan...

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Veröffentlicht in:	Biochemistry (Easton) 1996-05, Vol.35 (20), p.6425-6437
Hauptverfasser:	Sinev, Michael A, Sineva, Elena V, Ittah, Varda, Haas, Elisha
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylate Kinase - chemistry Adenylate Kinase - genetics Adenylate Kinase - metabolism Base Sequence Energy Transfer Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Oligodeoxyribonucleotides - genetics Point Mutation Protein Conformation Solutions Spectrometry, Fluorescence Tryptophan - chemistry
Online-Zugang:	Volltext
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