$Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium$

Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium

•Using a new defined medium to enriched cancer stem cells (CSC) content in colorectal cancer cell lines.•Morphological, functional and phenotypical characterization of CSC.•Rapid monitoring of CSC emergence with sedimentation field flow fractionation. The development of methods to enrich cell popula...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2014-07, Vol.963, p.40-46
Hauptverfasser:	Mélin, Carole, Perraud, Aurélie, Bounaix Morand du Puch, Christophe, Loum, Elodie, Giraud, Stéphanie, Cardot, Philippe, Jauberteau, Marie-Odile, Lautrette, Christophe, Battu, Serge, Mathonnet, Muriel
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Cancer Cancer stem cells Cell Adhesion Cell enrichment Cell Line, Tumor Cell Proliferation Cell Separation - instrumentation Colon Colorectal cancer Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Culture Culture Media - metabolism Defined medium Enrichment Fractionation Fractionation, Field Flow - instrumentation Humans In vitro testing Life Sciences Neoplastic Stem Cells - cytology Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Sedimentation Sedimentation field flow fractionation Stem cells
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