Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium
•Using a new defined medium to enriched cancer stem cells (CSC) content in colorectal cancer cell lines.•Morphological, functional and phenotypical characterization of CSC.•Rapid monitoring of CSC emergence with sedimentation field flow fractionation. The development of methods to enrich cell popula...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2014-07, Vol.963, p.40-46 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Mélin, Carole Perraud, Aurélie Bounaix Morand du Puch, Christophe Loum, Elodie Giraud, Stéphanie Cardot, Philippe Jauberteau, Marie-Odile Lautrette, Christophe Battu, Serge Mathonnet, Muriel |
| description | •Using a new defined medium to enriched cancer stem cells (CSC) content in colorectal cancer cell lines.•Morphological, functional and phenotypical characterization of CSC.•Rapid monitoring of CSC emergence with sedimentation field flow fractionation.
The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes. |
| doi_str_mv | 10.1016/j.jchromb.2014.05.039 |
| format | Article |
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The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes.</description><identifier>ISSN: 1570-0232</identifier><identifier>ISSN: 0378-4347</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2014.05.039</identifier><identifier>PMID: 24927420</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Apoptosis ; Cancer ; Cancer stem cells ; Cell Adhesion ; Cell enrichment ; Cell Line, Tumor ; Cell Proliferation ; Cell Separation - instrumentation ; Colon ; Colorectal cancer ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Culture ; Culture Media - metabolism ; Defined medium ; Enrichment ; Fractionation ; Fractionation, Field Flow - instrumentation ; Humans ; In vitro testing ; Life Sciences ; Neoplastic Stem Cells - cytology ; Neoplastic Stem Cells - metabolism ; Neoplastic Stem Cells - pathology ; Sedimentation ; Sedimentation field flow fractionation ; Stem cells</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2014-07, Vol.963, p.40-46</ispartof><rights>2014 Elsevier B.V.</rights><rights>Copyright © 2014 Elsevier B.V. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-9a17e8ef058f10105284dbb6e5a2dac4c2ce1abd83b14746a4e290c96d13283</citedby><cites>FETCH-LOGICAL-c432t-9a17e8ef058f10105284dbb6e5a2dac4c2ce1abd83b14746a4e290c96d13283</cites><orcidid>0000-0002-9127-3068 ; 0000-0001-7882-0613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2014.05.039$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24927420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02421473$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Mélin, Carole</creatorcontrib><creatorcontrib>Perraud, Aurélie</creatorcontrib><creatorcontrib>Bounaix Morand du Puch, Christophe</creatorcontrib><creatorcontrib>Loum, Elodie</creatorcontrib><creatorcontrib>Giraud, Stéphanie</creatorcontrib><creatorcontrib>Cardot, Philippe</creatorcontrib><creatorcontrib>Jauberteau, Marie-Odile</creatorcontrib><creatorcontrib>Lautrette, Christophe</creatorcontrib><creatorcontrib>Battu, Serge</creatorcontrib><creatorcontrib>Mathonnet, Muriel</creatorcontrib><title>Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>•Using a new defined medium to enriched cancer stem cells (CSC) content in colorectal cancer cell lines.•Morphological, functional and phenotypical characterization of CSC.•Rapid monitoring of CSC emergence with sedimentation field flow fractionation.
The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes.</description><subject>Apoptosis</subject><subject>Cancer</subject><subject>Cancer stem cells</subject><subject>Cell Adhesion</subject><subject>Cell enrichment</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cell Separation - instrumentation</subject><subject>Colon</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Culture</subject><subject>Culture Media - metabolism</subject><subject>Defined medium</subject><subject>Enrichment</subject><subject>Fractionation</subject><subject>Fractionation, Field Flow - instrumentation</subject><subject>Humans</subject><subject>In vitro testing</subject><subject>Life Sciences</subject><subject>Neoplastic Stem Cells - cytology</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Neoplastic Stem Cells - pathology</subject><subject>Sedimentation</subject><subject>Sedimentation field flow fractionation</subject><subject>Stem cells</subject><issn>1570-0232</issn><issn>0378-4347</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS1ERcvATwB5CYuk14-8VqiqKEUaiUW76M5ynGvGoyQe7GSqrvnjOM3QLaxsXZ_jY5-PkA8McgasvNzne7MLfmhzDkzmUOQgmlfkgtWVyERVPrxO-6KCDLjg5-RtjHsAVkEl3pBzLhteSQ4X5Pcddm7AcdKT8yO1DvuO2t4_Uhu0WWbrweBHN_ngxp_UW-pGenRT8BTH4Mxu8S8zo0eDgcYJB2qw7yNtn2g8oHHWGRoxzENmAyI1cz_NAemQwufhHTmzuo_4_rRuyN3N1_vr22z749v366ttZqTgU9ZoVmGNForapgqg4LXs2rbEQvNOG2m4QabbrhYtk5UstUTegGnKjgleiw35vN660706BDfo8KS8dur2aquWGXDJk1EcWdJ-WrWH4H_NGCc1uLj8SI_o56hYUTQVZ7Vo_kOaegZRJiwbUqxSE3yMAe3LMxioBaraqxNUtUBVUCh4jvh4ipjb1NiL6y_FJPiyCjC1d3QYVDQOE4vOBTST6rz7R8QfgJu3Og</recordid><startdate>20140715</startdate><enddate>20140715</enddate><creator>Mélin, Carole</creator><creator>Perraud, Aurélie</creator><creator>Bounaix Morand du Puch, Christophe</creator><creator>Loum, Elodie</creator><creator>Giraud, Stéphanie</creator><creator>Cardot, Philippe</creator><creator>Jauberteau, Marie-Odile</creator><creator>Lautrette, Christophe</creator><creator>Battu, Serge</creator><creator>Mathonnet, Muriel</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9127-3068</orcidid><orcidid>https://orcid.org/0000-0001-7882-0613</orcidid></search><sort><creationdate>20140715</creationdate><title>Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium</title><author>Mélin, Carole ; Perraud, Aurélie ; Bounaix Morand du Puch, Christophe ; Loum, Elodie ; Giraud, Stéphanie ; Cardot, Philippe ; Jauberteau, Marie-Odile ; Lautrette, Christophe ; Battu, Serge ; Mathonnet, Muriel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-9a17e8ef058f10105284dbb6e5a2dac4c2ce1abd83b14746a4e290c96d13283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Apoptosis</topic><topic>Cancer</topic><topic>Cancer stem cells</topic><topic>Cell Adhesion</topic><topic>Cell enrichment</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cell Separation - instrumentation</topic><topic>Colon</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Culture</topic><topic>Culture Media - metabolism</topic><topic>Defined medium</topic><topic>Enrichment</topic><topic>Fractionation</topic><topic>Fractionation, Field Flow - instrumentation</topic><topic>Humans</topic><topic>In vitro testing</topic><topic>Life Sciences</topic><topic>Neoplastic Stem Cells - cytology</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Neoplastic Stem Cells - pathology</topic><topic>Sedimentation</topic><topic>Sedimentation field flow fractionation</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mélin, Carole</creatorcontrib><creatorcontrib>Perraud, Aurélie</creatorcontrib><creatorcontrib>Bounaix Morand du Puch, Christophe</creatorcontrib><creatorcontrib>Loum, Elodie</creatorcontrib><creatorcontrib>Giraud, Stéphanie</creatorcontrib><creatorcontrib>Cardot, Philippe</creatorcontrib><creatorcontrib>Jauberteau, Marie-Odile</creatorcontrib><creatorcontrib>Lautrette, Christophe</creatorcontrib><creatorcontrib>Battu, Serge</creatorcontrib><creatorcontrib>Mathonnet, Muriel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of chromatography. 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B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2014-07-15</date><risdate>2014</risdate><volume>963</volume><spage>40</spage><epage>46</epage><pages>40-46</pages><issn>1570-0232</issn><issn>0378-4347</issn><eissn>1873-376X</eissn><abstract>•Using a new defined medium to enriched cancer stem cells (CSC) content in colorectal cancer cell lines.•Morphological, functional and phenotypical characterization of CSC.•Rapid monitoring of CSC emergence with sedimentation field flow fractionation.
The development of methods to enrich cell populations for cancer stem cells (CSC) is urgently needed to help understand tumor progression, therapeutic escape and to evaluate new drugs, in particular for colorectal cancer (CRC). In this work, we describe the in vitro use of OncoMiD for colon, a CRC-specific primary cell culture medium, to enrich CRC cell lines in CSC. Sedimentation field flow fractionation (SdFFF) was used to monitor the evolution of subpopulations composition. In these models, medium induced a loss of adherence properties associated with a balance between proliferation and apoptosis rates and, more important, an increased expression of relevant CSC markers, leading to specific SdFFF elution profile changes.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>24927420</pmid><doi>10.1016/j.jchromb.2014.05.039</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-9127-3068</orcidid><orcidid>https://orcid.org/0000-0001-7882-0613</orcidid></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2014-07, Vol.963, p.40-46 |
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| subjects | Apoptosis Cancer Cancer stem cells Cell Adhesion Cell enrichment Cell Line, Tumor Cell Proliferation Cell Separation - instrumentation Colon Colorectal cancer Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Culture Culture Media - metabolism Defined medium Enrichment Fractionation Fractionation, Field Flow - instrumentation Humans In vitro testing Life Sciences Neoplastic Stem Cells - cytology Neoplastic Stem Cells - metabolism Neoplastic Stem Cells - pathology Sedimentation Sedimentation field flow fractionation Stem cells |
| title | Sedimentation field flow fractionation monitoring of in vitro enrichment in cancer stem cells by specific serum-free culture medium |
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