Effects of membrane-lipid composition on nascent protein translocation induced by heat and ethanol in Escherichia coli
1. 1.|In fatty acid supplemented E. coli auxotrophs the degree of saturation in acyl chains 18 carbons long did not influence the translocation of protein to the outer membrane under hyperthermal stress. However, in cells grown in medium supplemented with 20-carbon fatty acids the degree of saturati...
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| Veröffentlicht in: | Journal of thermal biology 1994, Vol.19 (2), p.111-122 |
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| container_title | Journal of thermal biology |
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| creator | Stowell, Michael H.B. McClard, Ronald W. Yatvin, Milton B. |
| description | 1.
1.|In fatty acid supplemented
E. coli auxotrophs the degree of saturation in acyl chains 18 carbons long did not influence the translocation of protein to the outer membrane under hyperthermal stress. However, in cells grown in medium supplemented with 20-carbon fatty acids the degree of saturation markedly affected protein translocation and acute exposure of cells to 6% (v/v) ethanol mimicked the effects of hyperthermic treatment.
2.
2.|
31PNMR studies revealed that whole membranes exhibited lamellar properties at 25°C but at growth temperature (39°C) and above they exhibited a mainly isotropic nature irrespective of fatty acid supplement. At temperatures greater than 40°C, a new isotropic component (
ca 20 ppm downfield) was observed. This isotropic component does not appear to arise from any typical phosphorus metabolite or lipid phase.
3.
3.|The role of lipid composition in protein translocation induced by ethanol and heat stress are discussed and a hypothesis for heat shock activation is presented. |
| doi_str_mv | 10.1016/0306-4565(94)90058-2 |
| format | Article |
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1.|In fatty acid supplemented
E. coli auxotrophs the degree of saturation in acyl chains 18 carbons long did not influence the translocation of protein to the outer membrane under hyperthermal stress. However, in cells grown in medium supplemented with 20-carbon fatty acids the degree of saturation markedly affected protein translocation and acute exposure of cells to 6% (v/v) ethanol mimicked the effects of hyperthermic treatment.
2.
2.|
31PNMR studies revealed that whole membranes exhibited lamellar properties at 25°C but at growth temperature (39°C) and above they exhibited a mainly isotropic nature irrespective of fatty acid supplement. At temperatures greater than 40°C, a new isotropic component (
ca 20 ppm downfield) was observed. This isotropic component does not appear to arise from any typical phosphorus metabolite or lipid phase.
3.
3.|The role of lipid composition in protein translocation induced by ethanol and heat stress are discussed and a hypothesis for heat shock activation is presented.</description><identifier>ISSN: 0306-4565</identifier><identifier>EISSN: 1879-0992</identifier><identifier>DOI: 10.1016/0306-4565(94)90058-2</identifier><identifier>CODEN: JTBIDS</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Biological and medical sciences ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Heat-stress ; membrane-lipids ; protein-translocation ; Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Journal of thermal biology, 1994, Vol.19 (2), p.111-122</ispartof><rights>1994</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-55dce6c73d2e9f2f55a50556ea2328c0c937343574d71e6a13f013f84ef019e33</citedby><cites>FETCH-LOGICAL-c364t-55dce6c73d2e9f2f55a50556ea2328c0c937343574d71e6a13f013f84ef019e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0306-4565(94)90058-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27910,27911,27912,45982</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4095680$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Stowell, Michael H.B.</creatorcontrib><creatorcontrib>McClard, Ronald W.</creatorcontrib><creatorcontrib>Yatvin, Milton B.</creatorcontrib><title>Effects of membrane-lipid composition on nascent protein translocation induced by heat and ethanol in Escherichia coli</title><title>Journal of thermal biology</title><description>1.
1.|In fatty acid supplemented
E. coli auxotrophs the degree of saturation in acyl chains 18 carbons long did not influence the translocation of protein to the outer membrane under hyperthermal stress. However, in cells grown in medium supplemented with 20-carbon fatty acids the degree of saturation markedly affected protein translocation and acute exposure of cells to 6% (v/v) ethanol mimicked the effects of hyperthermic treatment.
2.
2.|
31PNMR studies revealed that whole membranes exhibited lamellar properties at 25°C but at growth temperature (39°C) and above they exhibited a mainly isotropic nature irrespective of fatty acid supplement. At temperatures greater than 40°C, a new isotropic component (
ca 20 ppm downfield) was observed. This isotropic component does not appear to arise from any typical phosphorus metabolite or lipid phase.
3.
3.|The role of lipid composition in protein translocation induced by ethanol and heat stress are discussed and a hypothesis for heat shock activation is presented.</description><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heat-stress</subject><subject>membrane-lipids</subject><subject>protein-translocation</subject><subject>Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0306-4565</issn><issn>1879-0992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kEFrHSEQx6U00Nek36AHD6W0h210dXbXS6GE1yYQ6CU9i3FHnmVXX9UXyLfPvLyQY0GZg7-Z8f9j7KMU36SQw6VQYug0DPDF6K9GCJi6_g3byGk0nTCmf8s2r8g79r7Wv0JIUCA27GEbAvpWeQ58xfW-uITdEvdx5j6v-1xjizlxOslVj6nxfckNY-KN0Lpk756BmOaDx5nfP_IdusZdmjm2nUt5oTe-rX6HJfpddDR3iRfsLLil4oeXes7-_NzeXV13t79_3Vz9uO28GnTrAGaPgx_V3KMJfQBwIAAGdL3qJy-8UaPSCkY9jxIHJ1UQdCeNVA0qdc4-n-bSr_8dsDa7RoqxLBQzH6qVAEaPYAjUJ9CXXGvBYPclrq48WinsUbI9GrRHg9Zo-yzZ9tT26WU-2XFLICc-1tdeLQwMkyDs-wlDyvoQsdjqIyYSFgvZt3OO_9_zBI4NkXQ</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Stowell, Michael H.B.</creator><creator>McClard, Ronald W.</creator><creator>Yatvin, Milton B.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>1994</creationdate><title>Effects of membrane-lipid composition on nascent protein translocation induced by heat and ethanol in Escherichia coli</title><author>Stowell, Michael H.B. ; McClard, Ronald W. ; Yatvin, Milton B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-55dce6c73d2e9f2f55a50556ea2328c0c937343574d71e6a13f013f84ef019e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Biological and medical sciences</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heat-stress</topic><topic>membrane-lipids</topic><topic>protein-translocation</topic><topic>Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stowell, Michael H.B.</creatorcontrib><creatorcontrib>McClard, Ronald W.</creatorcontrib><creatorcontrib>Yatvin, Milton B.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Journal of thermal biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stowell, Michael H.B.</au><au>McClard, Ronald W.</au><au>Yatvin, Milton B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of membrane-lipid composition on nascent protein translocation induced by heat and ethanol in Escherichia coli</atitle><jtitle>Journal of thermal biology</jtitle><date>1994</date><risdate>1994</risdate><volume>19</volume><issue>2</issue><spage>111</spage><epage>122</epage><pages>111-122</pages><issn>0306-4565</issn><eissn>1879-0992</eissn><coden>JTBIDS</coden><abstract>1.
1.|In fatty acid supplemented
E. coli auxotrophs the degree of saturation in acyl chains 18 carbons long did not influence the translocation of protein to the outer membrane under hyperthermal stress. However, in cells grown in medium supplemented with 20-carbon fatty acids the degree of saturation markedly affected protein translocation and acute exposure of cells to 6% (v/v) ethanol mimicked the effects of hyperthermic treatment.
2.
2.|
31PNMR studies revealed that whole membranes exhibited lamellar properties at 25°C but at growth temperature (39°C) and above they exhibited a mainly isotropic nature irrespective of fatty acid supplement. At temperatures greater than 40°C, a new isotropic component (
ca 20 ppm downfield) was observed. This isotropic component does not appear to arise from any typical phosphorus metabolite or lipid phase.
3.
3.|The role of lipid composition in protein translocation induced by ethanol and heat stress are discussed and a hypothesis for heat shock activation is presented.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/0306-4565(94)90058-2</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of thermal biology, 1994, Vol.19 (2), p.111-122 |
| issn | 0306-4565 1879-0992 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15594759 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings |
| subjects | Biological and medical sciences Escherichia coli Fundamental and applied biological sciences. Psychology Heat-stress membrane-lipids protein-translocation Thermoregulation. Hibernation. Estivation. Ecophysiology and environmental effects Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | Effects of membrane-lipid composition on nascent protein translocation induced by heat and ethanol in Escherichia coli |
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