PetCR46, a gene which is essential for respiration and integrity of the mitochondrial genome
In the frame of the European Pilot Project for the functional analysis of newly discovered open reading frames (ORFs) from Saccharomyces cerevisiae chromosome III, we have deleted entirely the YCR46C ORF by a one‐step polymerase chain reaction method and replaced it by the HIS3 marker in the strain...
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| Veröffentlicht in: | Yeast (Chichester, England) England), 1996-05, Vol.12 (6), p.577-582 |
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| container_title | Yeast (Chichester, England) |
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| creator | Coppée, J.‐Y Rieger, K.‐J Kaniak, A Di Rago, J.‐P Groudinsky, O Slonimski, P.P |
| description | In the frame of the European Pilot Project for the functional analysis of newly discovered open reading frames (ORFs) from Saccharomyces cerevisiae chromosome III, we have deleted entirely the YCR46C ORF by a one‐step polymerase chain reaction method and replaced it by the HIS3 marker in the strain W303. The deletion has been checked by meiotic segregation and Southern blot analyses. Characterization of the deleted strain indicates that YCR46C is essential for respiration and maintenance of the mitochondrial genome since its deletion leads to the appearance of 100% of cytoplasmic petites. Hybridization with molecular probes from mtDNA of individual clones of such petites showed that about 50% did hybridize (rho⁻ clones) while others did not (possibly rho° clones). The wild‐type gene has been cloned and shown to complement the deletion. The gene, which probably codes for a mitochondrial ribosomal protein, has been called petCR46. |
| doi_str_mv | 10.1002/(SICI)1097-0061(199605)12:6<577::AID-YEA950>3.0.CO;2-2 |
| format | Article |
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The deletion has been checked by meiotic segregation and Southern blot analyses. Characterization of the deleted strain indicates that YCR46C is essential for respiration and maintenance of the mitochondrial genome since its deletion leads to the appearance of 100% of cytoplasmic petites. Hybridization with molecular probes from mtDNA of individual clones of such petites showed that about 50% did hybridize (rho⁻ clones) while others did not (possibly rho° clones). The wild‐type gene has been cloned and shown to complement the deletion. The gene, which probably codes for a mitochondrial ribosomal protein, has been called petCR46.</description><identifier>ISSN: 0749-503X</identifier><identifier>EISSN: 1097-0061</identifier><identifier>DOI: 10.1002/(SICI)1097-0061(199605)12:6<577::AID-YEA950>3.0.CO;2-2</identifier><identifier>PMID: 8771712</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Base Sequence ; Blotting, Southern ; Chromosome III ; DNA, Mitochondrial - genetics ; Genome ; Meiosis ; mitochondrial DNA ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; nuclear petite ; Polymerase Chain Reaction ; Reading Frames - genetics ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - cytology ; Saccharomyces cerevisiae - genetics ; Sequence Deletion ; YCR46C</subject><ispartof>Yeast (Chichester, England), 1996-05, Vol.12 (6), p.577-582</ispartof><rights>Copyright © 1996 John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4990-39dc766d49bed6d2c6ba1f95c0ada7617869f771a552eca57078c92ffdff3ad33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291097-0061%28199605%2912%3A6%3C577%3A%3AAID-YEA950%3E3.0.CO%3B2-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291097-0061%28199605%2912%3A6%3C577%3A%3AAID-YEA950%3E3.0.CO%3B2-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8771712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coppée, J.‐Y</creatorcontrib><creatorcontrib>Rieger, K.‐J</creatorcontrib><creatorcontrib>Kaniak, A</creatorcontrib><creatorcontrib>Di Rago, J.‐P</creatorcontrib><creatorcontrib>Groudinsky, O</creatorcontrib><creatorcontrib>Slonimski, P.P</creatorcontrib><title>PetCR46, a gene which is essential for respiration and integrity of the mitochondrial genome</title><title>Yeast (Chichester, England)</title><addtitle>Yeast</addtitle><description>In the frame of the European Pilot Project for the functional analysis of newly discovered open reading frames (ORFs) from Saccharomyces cerevisiae chromosome III, we have deleted entirely the YCR46C ORF by a one‐step polymerase chain reaction method and replaced it by the HIS3 marker in the strain W303. The deletion has been checked by meiotic segregation and Southern blot analyses. Characterization of the deleted strain indicates that YCR46C is essential for respiration and maintenance of the mitochondrial genome since its deletion leads to the appearance of 100% of cytoplasmic petites. Hybridization with molecular probes from mtDNA of individual clones of such petites showed that about 50% did hybridize (rho⁻ clones) while others did not (possibly rho° clones). The wild‐type gene has been cloned and shown to complement the deletion. The gene, which probably codes for a mitochondrial ribosomal protein, has been called petCR46.</description><subject>Base Sequence</subject><subject>Blotting, Southern</subject><subject>Chromosome III</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Genome</subject><subject>Meiosis</subject><subject>mitochondrial DNA</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Site-Directed</subject><subject>nuclear petite</subject><subject>Polymerase Chain Reaction</subject><subject>Reading Frames - genetics</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - cytology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Sequence Deletion</subject><subject>YCR46C</subject><issn>0749-503X</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkWFrFDEQhoMo9az-BDGfpAX3TLKbZHNK4VirHhROrJUKwpDLJr3I7uZM9ij3791lj35R8FMgM_O8wzMIXVAyp4Swt2fXq2p1TomSGSGCnlGlBOHnlC3Eey7lYrFcfch-XC4VJxf5nMyr9TuWsUdo9jDyGM2ILFTGSX77FD1L6RchlHJWnqCTUkoqKZuhn19sX30txBus8Z3tLL7ferPFPmGbku16rxvsQsTRpp2Puvehw7qrse96exd9f8DB4X5rcev7YLahq-M4MqBCa5-jJ043yb44vqfo5uPlt-pzdrX-tKqWV5kplCJZrmojhagLtbG1qJkRG02d4oboWktBZSmUGxbWnDNrNJdElkYx52rncl3n-Sl6PXF3Mfze29RD65OxTaM7G_YJKOeqkEU5NH6fGk0MKUXrYBd9q-MBKIFRO8CoHUaHMDqESTtQBgIG7QCDdpi0Qw4EqjUwYAP45XGD_aa19QP26Hmo3071e9_Yw1-p_wn9Z-bxZ0C_mtBOB9DDTRLcXDNCc0IVLUrJ8j-5JKcz</recordid><startdate>199605</startdate><enddate>199605</enddate><creator>Coppée, J.‐Y</creator><creator>Rieger, K.‐J</creator><creator>Kaniak, A</creator><creator>Di Rago, J.‐P</creator><creator>Groudinsky, O</creator><creator>Slonimski, P.P</creator><general>John Wiley & Sons, Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>199605</creationdate><title>PetCR46, a gene which is essential for respiration and integrity of the mitochondrial genome</title><author>Coppée, J.‐Y ; Rieger, K.‐J ; Kaniak, A ; Di Rago, J.‐P ; Groudinsky, O ; Slonimski, P.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4990-39dc766d49bed6d2c6ba1f95c0ada7617869f771a552eca57078c92ffdff3ad33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Sequence</topic><topic>Blotting, Southern</topic><topic>Chromosome III</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Genome</topic><topic>Meiosis</topic><topic>mitochondrial DNA</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Site-Directed</topic><topic>nuclear petite</topic><topic>Polymerase Chain Reaction</topic><topic>Reading Frames - genetics</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - cytology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Sequence Deletion</topic><topic>YCR46C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coppée, J.‐Y</creatorcontrib><creatorcontrib>Rieger, K.‐J</creatorcontrib><creatorcontrib>Kaniak, A</creatorcontrib><creatorcontrib>Di Rago, J.‐P</creatorcontrib><creatorcontrib>Groudinsky, O</creatorcontrib><creatorcontrib>Slonimski, P.P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coppée, J.‐Y</au><au>Rieger, K.‐J</au><au>Kaniak, A</au><au>Di Rago, J.‐P</au><au>Groudinsky, O</au><au>Slonimski, P.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PetCR46, a gene which is essential for respiration and integrity of the mitochondrial genome</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>1996-05</date><risdate>1996</risdate><volume>12</volume><issue>6</issue><spage>577</spage><epage>582</epage><pages>577-582</pages><issn>0749-503X</issn><eissn>1097-0061</eissn><abstract>In the frame of the European Pilot Project for the functional analysis of newly discovered open reading frames (ORFs) from Saccharomyces cerevisiae chromosome III, we have deleted entirely the YCR46C ORF by a one‐step polymerase chain reaction method and replaced it by the HIS3 marker in the strain W303. The deletion has been checked by meiotic segregation and Southern blot analyses. Characterization of the deleted strain indicates that YCR46C is essential for respiration and maintenance of the mitochondrial genome since its deletion leads to the appearance of 100% of cytoplasmic petites. Hybridization with molecular probes from mtDNA of individual clones of such petites showed that about 50% did hybridize (rho⁻ clones) while others did not (possibly rho° clones). The wild‐type gene has been cloned and shown to complement the deletion. The gene, which probably codes for a mitochondrial ribosomal protein, has been called petCR46.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8771712</pmid><doi>10.1002/(SICI)1097-0061(199605)12:6<577::AID-YEA950>3.0.CO;2-2</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Yeast (Chichester, England), 1996-05, Vol.12 (6), p.577-582 |
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| source | Wiley-Blackwell Free Backfiles(OpenAccess); Wiley Online Library - AutoHoldings Journals; MEDLINE; EZB Electronic Journals Library |
| subjects | Base Sequence Blotting, Southern Chromosome III DNA, Mitochondrial - genetics Genome Meiosis mitochondrial DNA Molecular Sequence Data Mutagenesis, Site-Directed nuclear petite Polymerase Chain Reaction Reading Frames - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - cytology Saccharomyces cerevisiae - genetics Sequence Deletion YCR46C |
| title | PetCR46, a gene which is essential for respiration and integrity of the mitochondrial genome |
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