Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes
We previously described avian leukosis virus-based packaging cell lines that produce stocks of retroviral vectors in which replication-competent viruses were not detectable. However, following infection of target cells with these retroviral stocks, we recently obtained colonies resulting from the tr...
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| Veröffentlicht in: | Journal of Virology 1996-08, Vol.70 (8), p.5651-5657 |
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| container_title | Journal of Virology |
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| creator | Girod, A. (CNRS UMR, Villeurbanne, France.) Drynda, A Cosset, F.L Verdier, G Ronfort, C |
| description | We previously described avian leukosis virus-based packaging cell lines that produce stocks of retroviral vectors in which replication-competent viruses were not detectable. However, following infection of target cells with these retroviral stocks, we recently obtained colonies resulting from the transmission of recombinant genomes. Here, we have analyzed their genetic structure and shown that (i) each of them results from recombination between the packaging- and integration-defective transcomplementing genomes and the retroviral vector, (ii) recombination probably occurred during the reverse transcription step, involving strand switching of the reverse transcription growing point from the infectious retroviral vector to the transcomplementing RNA; and (iii) sequence identity and nonhomologous sequences were both used for the strand switching |
| doi_str_mv | 10.1128/jvi.70.8.5651-5657.1996 |
| format | Article |
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(CNRS UMR, Villeurbanne, France.) ; Drynda, A ; Cosset, F.L ; Verdier, G ; Ronfort, C</creator><creatorcontrib>Girod, A. (CNRS UMR, Villeurbanne, France.) ; Drynda, A ; Cosset, F.L ; Verdier, G ; Ronfort, C</creatorcontrib><description>We previously described avian leukosis virus-based packaging cell lines that produce stocks of retroviral vectors in which replication-competent viruses were not detectable. However, following infection of target cells with these retroviral stocks, we recently obtained colonies resulting from the transmission of recombinant genomes. Here, we have analyzed their genetic structure and shown that (i) each of them results from recombination between the packaging- and integration-defective transcomplementing genomes and the retroviral vector, (ii) recombination probably occurred during the reverse transcription step, involving strand switching of the reverse transcription growing point from the infectious retroviral vector to the transcomplementing RNA; and (iii) sequence identity and nonhomologous sequences were both used for the strand switching</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.70.8.5651-5657.1996</identifier><identifier>PMID: 8764082</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>ADN ; AGENTES ANTINEOPLASTICOS ; Animals ; ANTIBIOTICOS ; ANTIBIOTIQUE ; Avian Leukosis Virus - genetics ; Base Sequence ; BIOSINTESIS ; BIOSYNTHESE ; Defective Viruses - genetics ; GENOMAS ; GENOME ; Genome, Viral ; Life Sciences ; MARCADORES GENETICOS ; MARQUEUR GENETIQUE ; MEDICAMENT ; MEDICAMENT CYTOSTATIQUE ; MEDICAMENTOS ; Microbiology and Parasitology ; Molecular Sequence Data ; MUTANT ; MUTANTES ; ONCOVIRUS AVIAIRE ; ONCOVIRUS AVIAR ; RECOMBINACION ; RECOMBINAISON ; Recombination, Genetic ; RESISTANCE AUX PRODUITS CHIMIQUES ; RESISTENCIA A PRODUCTOS QUIMICOS ; RETROVIRIDAE ; SECUENCIA NUCLEOTIDICA ; SEQUENCE NUCLEOTIDIQUE ; VECTEUR DE MALADIE ; VECTORES ; Virology</subject><ispartof>Journal of Virology, 1996-08, Vol.70 (8), p.5651-5657</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4906-81e3b2724002871cd5c8b9ac49543ebe8d198ee9b3aec92f83469c7a675f2ec63</citedby><orcidid>0000-0001-8842-3726</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC190528/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC190528/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8764082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02686519$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Girod, A. (CNRS UMR, Villeurbanne, France.)</creatorcontrib><creatorcontrib>Drynda, A</creatorcontrib><creatorcontrib>Cosset, F.L</creatorcontrib><creatorcontrib>Verdier, G</creatorcontrib><creatorcontrib>Ronfort, C</creatorcontrib><title>Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>We previously described avian leukosis virus-based packaging cell lines that produce stocks of retroviral vectors in which replication-competent viruses were not detectable. However, following infection of target cells with these retroviral stocks, we recently obtained colonies resulting from the transmission of recombinant genomes. 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(CNRS UMR, Villeurbanne, France.)</creator><creator>Drynda, A</creator><creator>Cosset, F.L</creator><creator>Verdier, G</creator><creator>Ronfort, C</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8842-3726</orcidid></search><sort><creationdate>19960801</creationdate><title>Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes</title><author>Girod, A. (CNRS UMR, Villeurbanne, France.) ; Drynda, A ; Cosset, F.L ; Verdier, G ; Ronfort, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4906-81e3b2724002871cd5c8b9ac49543ebe8d198ee9b3aec92f83469c7a675f2ec63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADN</topic><topic>AGENTES ANTINEOPLASTICOS</topic><topic>Animals</topic><topic>ANTIBIOTICOS</topic><topic>ANTIBIOTIQUE</topic><topic>Avian Leukosis Virus - genetics</topic><topic>Base Sequence</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>Defective Viruses - genetics</topic><topic>GENOMAS</topic><topic>GENOME</topic><topic>Genome, Viral</topic><topic>Life Sciences</topic><topic>MARCADORES GENETICOS</topic><topic>MARQUEUR GENETIQUE</topic><topic>MEDICAMENT</topic><topic>MEDICAMENT CYTOSTATIQUE</topic><topic>MEDICAMENTOS</topic><topic>Microbiology and Parasitology</topic><topic>Molecular Sequence Data</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>ONCOVIRUS AVIAIRE</topic><topic>ONCOVIRUS AVIAR</topic><topic>RECOMBINACION</topic><topic>RECOMBINAISON</topic><topic>Recombination, Genetic</topic><topic>RESISTANCE AUX PRODUITS CHIMIQUES</topic><topic>RESISTENCIA A PRODUCTOS QUIMICOS</topic><topic>RETROVIRIDAE</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>VECTEUR DE MALADIE</topic><topic>VECTORES</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Girod, A. (CNRS UMR, Villeurbanne, France.)</creatorcontrib><creatorcontrib>Drynda, A</creatorcontrib><creatorcontrib>Cosset, F.L</creatorcontrib><creatorcontrib>Verdier, G</creatorcontrib><creatorcontrib>Ronfort, C</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Girod, A. (CNRS UMR, Villeurbanne, France.)</au><au>Drynda, A</au><au>Cosset, F.L</au><au>Verdier, G</au><au>Ronfort, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>70</volume><issue>8</issue><spage>5651</spage><epage>5657</epage><pages>5651-5657</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>We previously described avian leukosis virus-based packaging cell lines that produce stocks of retroviral vectors in which replication-competent viruses were not detectable. However, following infection of target cells with these retroviral stocks, we recently obtained colonies resulting from the transmission of recombinant genomes. Here, we have analyzed their genetic structure and shown that (i) each of them results from recombination between the packaging- and integration-defective transcomplementing genomes and the retroviral vector, (ii) recombination probably occurred during the reverse transcription step, involving strand switching of the reverse transcription growing point from the infectious retroviral vector to the transcomplementing RNA; and (iii) sequence identity and nonhomologous sequences were both used for the strand switching</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>8764082</pmid><doi>10.1128/jvi.70.8.5651-5657.1996</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8842-3726</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-538X |
| ispartof | Journal of Virology, 1996-08, Vol.70 (8), p.5651-5657 |
| issn | 0022-538X 1098-5514 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ADN AGENTES ANTINEOPLASTICOS Animals ANTIBIOTICOS ANTIBIOTIQUE Avian Leukosis Virus - genetics Base Sequence BIOSINTESIS BIOSYNTHESE Defective Viruses - genetics GENOMAS GENOME Genome, Viral Life Sciences MARCADORES GENETICOS MARQUEUR GENETIQUE MEDICAMENT MEDICAMENT CYTOSTATIQUE MEDICAMENTOS Microbiology and Parasitology Molecular Sequence Data MUTANT MUTANTES ONCOVIRUS AVIAIRE ONCOVIRUS AVIAR RECOMBINACION RECOMBINAISON Recombination, Genetic RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA A PRODUCTOS QUIMICOS RETROVIRIDAE SECUENCIA NUCLEOTIDICA SEQUENCE NUCLEOTIDIQUE VECTEUR DE MALADIE VECTORES Virology |
| title | Homologous and nonhomologous retroviral recombinations are both involved in the transfer by infectious particles of defective avian leukosis virus-derived transcomplementing genomes |
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