Soluble, dye-labelled substrates for a micro-plate assay of proteinase activity
Gelatin, collagen and casein derivatives were covalently linked with Remazol Brilliant Blue R (RBB). New dye-labelled, soluble substrates were obtained, suitable for the detection and assay of proteinase activity. A reliable colorimetric micro-plate assay was designed, based on the precipitability o...
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| Veröffentlicht in: | Journal of microbiological methods 1996, Vol.25 (3), p.337-342 |
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| container_end_page | 342 |
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| container_issue | 3 |
| container_start_page | 337 |
| container_title | Journal of microbiological methods |
| container_volume | 25 |
| creator | Wolf, Gerhard A. Wirth, Stephan J. |
| description | Gelatin, collagen and casein derivatives were covalently linked with Remazol Brilliant Blue R (RBB). New dye-labelled,
soluble substrates were obtained, suitable for the detection and assay of proteinase activity. A reliable colorimetric micro-plate assay was designed, based on the precipitability of gelatin-RBB, collagen-RBB and casein-RBB from buffered solutions with hydrochloric acid. Additionally, a sensitive plate-clearing assay was developed for the detection and isolation of proteolytic microorganisms from mixed populations, or for the screening of pure cultures. |
| doi_str_mv | 10.1016/0167-7012(96)00005-X |
| format | Article |
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soluble substrates were obtained, suitable for the detection and assay of proteinase activity. A reliable colorimetric micro-plate assay was designed, based on the precipitability of gelatin-RBB, collagen-RBB and casein-RBB from buffered solutions with hydrochloric acid. Additionally, a sensitive plate-clearing assay was developed for the detection and isolation of proteolytic microorganisms from mixed populations, or for the screening of pure cultures.</description><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Casein</subject><subject>Collagen</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gelatin</subject><subject>Micro-plate assay</subject><subject>Microbiology</subject><subject>Plate-clearing assay</subject><subject>Proteinase</subject><subject>Remazol Brilliant Blue R</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouK7-Aw89iChYTZo0bS-CLH7Bwh5U2FtI0ilEuu2aSRf6701Z2aOByZDhmck7LyGXjN4zyuRDjCItKMtuKnlL48nT9RGZsbLI0pLn1TGZHZBTcob4TSnLuShnZPXRt4Np4S6pR0hbbaBtoU5wMBi8DoBJ0_tEJxtnfZ9u21hKNKIek75Jtr4P4DqNsWaD27kwnpOTRrcIF395Tr5enj8Xb-ly9fq-eFqmlksR0qpgUoAUhmfMijxjdbwbiNkwzXVuSmEKVhoBRjYQZUNemEZWgkpR80rwObnez40afgbAoDYObRSvO-gHVCzPq4zxMoJiD0b9iB4atfVuo_2oGFWTe2qyRk3WqCo-JvfUOrZd_c3XaHXbeN1Zh4dezrJKChqxxz0GcdedA6_QOugs1M6DDaru3f___AKGnYMF</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Wolf, Gerhard A.</creator><creator>Wirth, Stephan J.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1996</creationdate><title>Soluble, dye-labelled substrates for a micro-plate assay of proteinase activity</title><author>Wolf, Gerhard A. ; Wirth, Stephan J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-97164e64b321c4521dc45fe21db1a3a5b84b718b4eb6fe012e57bf694064d3943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Casein</topic><topic>Collagen</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gelatin</topic><topic>Micro-plate assay</topic><topic>Microbiology</topic><topic>Plate-clearing assay</topic><topic>Proteinase</topic><topic>Remazol Brilliant Blue R</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolf, Gerhard A.</creatorcontrib><creatorcontrib>Wirth, Stephan J.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolf, Gerhard A.</au><au>Wirth, Stephan J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Soluble, dye-labelled substrates for a micro-plate assay of proteinase activity</atitle><jtitle>Journal of microbiological methods</jtitle><date>1996</date><risdate>1996</risdate><volume>25</volume><issue>3</issue><spage>337</spage><epage>342</epage><pages>337-342</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>Gelatin, collagen and casein derivatives were covalently linked with Remazol Brilliant Blue R (RBB). New dye-labelled,
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| fulltext | fulltext |
| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 1996, Vol.25 (3), p.337-342 |
| issn | 0167-7012 1872-8359 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15592138 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Casein Collagen Fundamental and applied biological sciences. Psychology Gelatin Micro-plate assay Microbiology Plate-clearing assay Proteinase Remazol Brilliant Blue R |
| title | Soluble, dye-labelled substrates for a micro-plate assay of proteinase activity |
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