Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy
The objective of the study was to investigate transcriptomic profile of pig endometrium on Days 12 and 16 of pregnancy in comparison with the respective days of the estrous cycle. Labeled complementary DNA was hybridized to Porcine Long Oligo microarray containing 13,297 oligonucleotide probes, whic...
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| Veröffentlicht in: | Theriogenology 2014-10, Vol.82 (6), p.897-909 |
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| creator | Kiewisz, Jolanta Krawczynski, Kamil Lisowski, Pawel Blitek, Agnieszka Zwierzchowski, Lech Ziecik, Adam J. Kaczmarek, Monika M. |
| description | The objective of the study was to investigate transcriptomic profile of pig endometrium on Days 12 and 16 of pregnancy in comparison with the respective days of the estrous cycle. Labeled complementary DNA was hybridized to Porcine Long Oligo microarray containing 13,297 oligonucleotide probes, which represented complementary DNA and expressed sequence tags. Statistical analysis revealed 110 differentially expressed genes (DEGs) on Day 12 of pregnancy and 179 DEGs on Day 16 of pregnancy. In silico analysis of gene function and functionality networks revealed links between genes implicated in cell death and survival, protein synthesis, lipid metabolism, cellular movement, tissue development, and cell-to-cell signaling. On Day 12 of pregnancy, estrogen, transforming growth factor (TGF) β1, and fibroblast growth factor (FGF) 2, and on Day 16 of pregnancy, epidermal growth factor (EGF), insulin, interleukin 11 (IL-11), and FGF family members were indicated as possible upstream regulators of several DEGs. Obtained results showed changes in global endometrial gene expression at the time of maternal recognition of pregnancy and embryo implantation. Additionally, these data revealed signaling molecules, which together with E2, may evoke molecular changes in the uterus, leading to successful pregnancy establishment. |
| doi_str_mv | 10.1016/j.theriogenology.2014.07.009 |
| format | Article |
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Labeled complementary DNA was hybridized to Porcine Long Oligo microarray containing 13,297 oligonucleotide probes, which represented complementary DNA and expressed sequence tags. Statistical analysis revealed 110 differentially expressed genes (DEGs) on Day 12 of pregnancy and 179 DEGs on Day 16 of pregnancy. In silico analysis of gene function and functionality networks revealed links between genes implicated in cell death and survival, protein synthesis, lipid metabolism, cellular movement, tissue development, and cell-to-cell signaling. On Day 12 of pregnancy, estrogen, transforming growth factor (TGF) β1, and fibroblast growth factor (FGF) 2, and on Day 16 of pregnancy, epidermal growth factor (EGF), insulin, interleukin 11 (IL-11), and FGF family members were indicated as possible upstream regulators of several DEGs. Obtained results showed changes in global endometrial gene expression at the time of maternal recognition of pregnancy and embryo implantation. Additionally, these data revealed signaling molecules, which together with E2, may evoke molecular changes in the uterus, leading to successful pregnancy establishment.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2014.07.009</identifier><identifier>PMID: 25123632</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Computer Simulation ; Endometrium ; Endometrium - physiology ; Estrogens - metabolism ; Estrous cycle ; Estrous Cycle - physiology ; Female ; Gene Expression Regulation - physiology ; Microarray ; Pig ; Pregnancy ; Progesterone - metabolism ; Swine - physiology ; Transcriptome - physiology</subject><ispartof>Theriogenology, 2014-10, Vol.82 (6), p.897-909</ispartof><rights>2014 Elsevier Inc.</rights><rights>Copyright © 2014 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-6738efcb74b3b543c3cfe17d626c18c24181511244801c9dad3a33ad0d69bc873</citedby><cites>FETCH-LOGICAL-c456t-6738efcb74b3b543c3cfe17d626c18c24181511244801c9dad3a33ad0d69bc873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.theriogenology.2014.07.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25123632$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiewisz, Jolanta</creatorcontrib><creatorcontrib>Krawczynski, Kamil</creatorcontrib><creatorcontrib>Lisowski, Pawel</creatorcontrib><creatorcontrib>Blitek, Agnieszka</creatorcontrib><creatorcontrib>Zwierzchowski, Lech</creatorcontrib><creatorcontrib>Ziecik, Adam J.</creatorcontrib><creatorcontrib>Kaczmarek, Monika M.</creatorcontrib><title>Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>The objective of the study was to investigate transcriptomic profile of pig endometrium on Days 12 and 16 of pregnancy in comparison with the respective days of the estrous cycle. Labeled complementary DNA was hybridized to Porcine Long Oligo microarray containing 13,297 oligonucleotide probes, which represented complementary DNA and expressed sequence tags. Statistical analysis revealed 110 differentially expressed genes (DEGs) on Day 12 of pregnancy and 179 DEGs on Day 16 of pregnancy. In silico analysis of gene function and functionality networks revealed links between genes implicated in cell death and survival, protein synthesis, lipid metabolism, cellular movement, tissue development, and cell-to-cell signaling. On Day 12 of pregnancy, estrogen, transforming growth factor (TGF) β1, and fibroblast growth factor (FGF) 2, and on Day 16 of pregnancy, epidermal growth factor (EGF), insulin, interleukin 11 (IL-11), and FGF family members were indicated as possible upstream regulators of several DEGs. Obtained results showed changes in global endometrial gene expression at the time of maternal recognition of pregnancy and embryo implantation. Additionally, these data revealed signaling molecules, which together with E2, may evoke molecular changes in the uterus, leading to successful pregnancy establishment.</description><subject>Animals</subject><subject>Computer Simulation</subject><subject>Endometrium</subject><subject>Endometrium - physiology</subject><subject>Estrogens - metabolism</subject><subject>Estrous cycle</subject><subject>Estrous Cycle - physiology</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Microarray</subject><subject>Pig</subject><subject>Pregnancy</subject><subject>Progesterone - metabolism</subject><subject>Swine - physiology</subject><subject>Transcriptome - physiology</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEFv1DAQhS0EapelfwH50AOXBI-dOInUCyptQarEpZW4WY49Wbxk7cXOIvLv67Btpd56GmnmmzdvHiHnwEpgID9vy-kXRhc26MMYNnPJGVQla0rGujdkBW3TFYILeEtWuSMK2cHPU_I-pS1jTEgJJ-SU18CFFHxFft-ModcjzWpI8d8-YkoueLqPYXCj8xsaBroP0bhl7m3Y4RSdphn5qudEgVPtLQW5cNkYxTTFcEjUzGbE_7OsufHam_kDeTfoMeHZY12T--uru8tvxe2Pm--XX24LU9VyKmQjWhxM31S96OtKGGEGhMZKLg20hlfQQg3Aq6plYDqrrdBCaMus7HrTNmJNPh118xN_DtmQ2rlkcBy1x2xNQV13LO9zkdGLI2piSCnioPbR7XScFTC1xK226mXcaolbsUYt4a7Jx8dLh36H9nn5Kd8MXB8BzP_-dRhVMg69QesimknZ4F536QFR-JpJ</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Kiewisz, Jolanta</creator><creator>Krawczynski, Kamil</creator><creator>Lisowski, Pawel</creator><creator>Blitek, Agnieszka</creator><creator>Zwierzchowski, Lech</creator><creator>Ziecik, Adam J.</creator><creator>Kaczmarek, Monika M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy</title><author>Kiewisz, Jolanta ; Krawczynski, Kamil ; Lisowski, Pawel ; Blitek, Agnieszka ; Zwierzchowski, Lech ; Ziecik, Adam J. ; Kaczmarek, Monika M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-6738efcb74b3b543c3cfe17d626c18c24181511244801c9dad3a33ad0d69bc873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Computer Simulation</topic><topic>Endometrium</topic><topic>Endometrium - physiology</topic><topic>Estrogens - metabolism</topic><topic>Estrous cycle</topic><topic>Estrous Cycle - physiology</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Microarray</topic><topic>Pig</topic><topic>Pregnancy</topic><topic>Progesterone - metabolism</topic><topic>Swine - physiology</topic><topic>Transcriptome - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiewisz, Jolanta</creatorcontrib><creatorcontrib>Krawczynski, Kamil</creatorcontrib><creatorcontrib>Lisowski, Pawel</creatorcontrib><creatorcontrib>Blitek, Agnieszka</creatorcontrib><creatorcontrib>Zwierzchowski, Lech</creatorcontrib><creatorcontrib>Ziecik, Adam J.</creatorcontrib><creatorcontrib>Kaczmarek, Monika M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiewisz, Jolanta</au><au>Krawczynski, Kamil</au><au>Lisowski, Pawel</au><au>Blitek, Agnieszka</au><au>Zwierzchowski, Lech</au><au>Ziecik, Adam J.</au><au>Kaczmarek, Monika M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>82</volume><issue>6</issue><spage>897</spage><epage>909</epage><pages>897-909</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>The objective of the study was to investigate transcriptomic profile of pig endometrium on Days 12 and 16 of pregnancy in comparison with the respective days of the estrous cycle. Labeled complementary DNA was hybridized to Porcine Long Oligo microarray containing 13,297 oligonucleotide probes, which represented complementary DNA and expressed sequence tags. Statistical analysis revealed 110 differentially expressed genes (DEGs) on Day 12 of pregnancy and 179 DEGs on Day 16 of pregnancy. In silico analysis of gene function and functionality networks revealed links between genes implicated in cell death and survival, protein synthesis, lipid metabolism, cellular movement, tissue development, and cell-to-cell signaling. On Day 12 of pregnancy, estrogen, transforming growth factor (TGF) β1, and fibroblast growth factor (FGF) 2, and on Day 16 of pregnancy, epidermal growth factor (EGF), insulin, interleukin 11 (IL-11), and FGF family members were indicated as possible upstream regulators of several DEGs. Obtained results showed changes in global endometrial gene expression at the time of maternal recognition of pregnancy and embryo implantation. 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| subjects | Animals Computer Simulation Endometrium Endometrium - physiology Estrogens - metabolism Estrous cycle Estrous Cycle - physiology Female Gene Expression Regulation - physiology Microarray Pig Pregnancy Progesterone - metabolism Swine - physiology Transcriptome - physiology |
| title | Global gene expression profiling of porcine endometria on Days 12 and 16 of the estrous cycle and pregnancy |
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