Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities
Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb. were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-1...
Gespeichert in:
| Veröffentlicht in: | Natural toxins 1996, Vol.4 (3), p.117-121 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 121 |
|---|---|
| container_issue | 3 |
| container_start_page | 117 |
| container_title | Natural toxins |
| container_volume | 4 |
| creator | Nikulin, M. (University of Helsinki, Helsinki.) Lappalainen, S Pasanen, A.L Laamanen, I Veijalainen, P Berg, S Hintikka, E.L |
| description | Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb. were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-100%. F. sporotrichioides grew well on oat and wheat grains at RH 97-100% but grew less well at RH 84-88% and on straw. Toxin production was measured with three biological toxicity tests (cytotoxicity test, dermotoxicity test, and yeast cell toxicity test), with chemical analysis, and T-2 ELISA assay. Cytotoxicity and production of trichothecene mycotoxins were detected in all the samples incubated at all three RH levels. On oat and wheat grains, T-2 toxin, neosolaniol, and diacetoxyscirpenol were found, and on straw T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were determined. In the T-2 ELISA assay, all material samples were found to contain T-2 toxin. The cytotoxicity test was the most sensitive method for detecting biological toxicity of samples inoculated with fungus. The T-2 ELISA assay and chemical analysis were about equally sensitive to detect T-2 toxin in samples |
| doi_str_mv | 10.1002/19960403NT3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15588686</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15588686</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3334-e0b84299f7dc05b5871ad9136a78f461d4b6b7c4345f7ea4e0dbf152e1d0ecba3</originalsourceid><addsrcrecordid>eNp9kU-LFDEQxRtR1nX15E0QcvIirZU_3ek-yuCuyjKCO6vgJaSTyk50ujMmaXQ-gt_arD0snjxVFfV7D-pVVT2l8IoCsNe071sQwNcbfq86pQ1jtaRdf7_00LR1D1Q8rB6l9A0AesrZSXXSScF7zk6r36sw7nX0KUwkOGIxo8m-DCPmbbCJuBBJjt5sQ96iwQkT2cdgZ4OWDAdyPqeinkeS9iGGv6AP3haqeLhgLUaiJ0tuovZTIjoT653DiFMm2keynUdvffaYHlcPnN4lfHKsZ9X1-dvN6l19-fHi_erNZW0456JGGDrB-t5Ja6AZmk5SbctVrZadEy21YmgHaQQXjZOoBYIdXMkEqQU0g-Zn1YvFt5zxY8aU1eiTwd1OTxjmpGjTdF3btQV8uYAmhpQiOrWPftTxoCio2-DVP8EX-vnRdh5GtHfsMemyb5b9T7_Dw_-s1HrDWga3E4iiqxedTxl_3el0_K5ayWWjvqwvFL0SHz5_Wn1V68I_W3ing9I35bPq-qqXrAPg_A9nTKfC</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15588686</pqid></control><display><type>article</type><title>Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Nikulin, M. (University of Helsinki, Helsinki.) ; Lappalainen, S ; Pasanen, A.L ; Laamanen, I ; Veijalainen, P ; Berg, S ; Hintikka, E.L</creator><creatorcontrib>Nikulin, M. (University of Helsinki, Helsinki.) ; Lappalainen, S ; Pasanen, A.L ; Laamanen, I ; Veijalainen, P ; Berg, S ; Hintikka, E.L</creatorcontrib><description>Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb. were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-100%. F. sporotrichioides grew well on oat and wheat grains at RH 97-100% but grew less well at RH 84-88% and on straw. Toxin production was measured with three biological toxicity tests (cytotoxicity test, dermotoxicity test, and yeast cell toxicity test), with chemical analysis, and T-2 ELISA assay. Cytotoxicity and production of trichothecene mycotoxins were detected in all the samples incubated at all three RH levels. On oat and wheat grains, T-2 toxin, neosolaniol, and diacetoxyscirpenol were found, and on straw T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were determined. In the T-2 ELISA assay, all material samples were found to contain T-2 toxin. The cytotoxicity test was the most sensitive method for detecting biological toxicity of samples inoculated with fungus. The T-2 ELISA assay and chemical analysis were about equally sensitive to detect T-2 toxin in samples</description><identifier>ISSN: 1056-9014</identifier><identifier>EISSN: 1522-7189</identifier><identifier>DOI: 10.1002/19960403NT3</identifier><identifier>PMID: 8743932</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>ALIMENT POUR ANIMAUX ; Animal Feed - microbiology ; Animals ; Avena - microbiology ; Carbon Dioxide - analysis ; Cats ; Cell Line ; Chemical analysis ; Cytotoxicity ; Cytotoxins - chemistry ; Cytotoxins - isolation & purification ; Cytotoxins - metabolism ; Cytotoxins - toxicity ; Dermotoxicity ; Dermotoxins - chemistry ; Dermotoxins - isolation & purification ; Dermotoxins - metabolism ; Dermotoxins - toxicity ; Enzyme-Linked Immunosorbent Assay ; Food Microbiology ; FUSARIUM ; Fusarium - growth & development ; Fusarium - metabolism ; Fusarium sporotrichioides ; Gas Chromatography-Mass Spectrometry ; GRAIN ; GRANOS ; Humidity ; Lung - cytology ; Lung - drug effects ; Lung - embryology ; PIENSOS ; Poaceae - microbiology ; Rabbits ; Relative humidity of air ; Saccharomyces cerevisiae - drug effects ; Skin - drug effects ; Species Specificity ; T-2 ELISA ; T-2 toxin ; TRICHOTHECENE ; TRICHOTHECENES ; Trichothecenes - chemistry ; Trichothecenes - isolation & purification ; Trichothecenes - metabolism ; Trichothecenes - toxicity ; Triticum - microbiology ; Yeast cell toxicity test</subject><ispartof>Natural toxins, 1996, Vol.4 (3), p.117-121</ispartof><rights>Copyright © 1996 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3334-e0b84299f7dc05b5871ad9136a78f461d4b6b7c4345f7ea4e0dbf152e1d0ecba3</citedby><cites>FETCH-LOGICAL-c3334-e0b84299f7dc05b5871ad9136a78f461d4b6b7c4345f7ea4e0dbf152e1d0ecba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F19960403NT3$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F19960403NT3$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,4010,27904,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8743932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nikulin, M. (University of Helsinki, Helsinki.)</creatorcontrib><creatorcontrib>Lappalainen, S</creatorcontrib><creatorcontrib>Pasanen, A.L</creatorcontrib><creatorcontrib>Laamanen, I</creatorcontrib><creatorcontrib>Veijalainen, P</creatorcontrib><creatorcontrib>Berg, S</creatorcontrib><creatorcontrib>Hintikka, E.L</creatorcontrib><title>Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities</title><title>Natural toxins</title><addtitle>Nat. Toxins</addtitle><description>Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb. were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-100%. F. sporotrichioides grew well on oat and wheat grains at RH 97-100% but grew less well at RH 84-88% and on straw. Toxin production was measured with three biological toxicity tests (cytotoxicity test, dermotoxicity test, and yeast cell toxicity test), with chemical analysis, and T-2 ELISA assay. Cytotoxicity and production of trichothecene mycotoxins were detected in all the samples incubated at all three RH levels. On oat and wheat grains, T-2 toxin, neosolaniol, and diacetoxyscirpenol were found, and on straw T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were determined. In the T-2 ELISA assay, all material samples were found to contain T-2 toxin. The cytotoxicity test was the most sensitive method for detecting biological toxicity of samples inoculated with fungus. The T-2 ELISA assay and chemical analysis were about equally sensitive to detect T-2 toxin in samples</description><subject>ALIMENT POUR ANIMAUX</subject><subject>Animal Feed - microbiology</subject><subject>Animals</subject><subject>Avena - microbiology</subject><subject>Carbon Dioxide - analysis</subject><subject>Cats</subject><subject>Cell Line</subject><subject>Chemical analysis</subject><subject>Cytotoxicity</subject><subject>Cytotoxins - chemistry</subject><subject>Cytotoxins - isolation & purification</subject><subject>Cytotoxins - metabolism</subject><subject>Cytotoxins - toxicity</subject><subject>Dermotoxicity</subject><subject>Dermotoxins - chemistry</subject><subject>Dermotoxins - isolation & purification</subject><subject>Dermotoxins - metabolism</subject><subject>Dermotoxins - toxicity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Food Microbiology</subject><subject>FUSARIUM</subject><subject>Fusarium - growth & development</subject><subject>Fusarium - metabolism</subject><subject>Fusarium sporotrichioides</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>GRAIN</subject><subject>GRANOS</subject><subject>Humidity</subject><subject>Lung - cytology</subject><subject>Lung - drug effects</subject><subject>Lung - embryology</subject><subject>PIENSOS</subject><subject>Poaceae - microbiology</subject><subject>Rabbits</subject><subject>Relative humidity of air</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Skin - drug effects</subject><subject>Species Specificity</subject><subject>T-2 ELISA</subject><subject>T-2 toxin</subject><subject>TRICHOTHECENE</subject><subject>TRICHOTHECENES</subject><subject>Trichothecenes - chemistry</subject><subject>Trichothecenes - isolation & purification</subject><subject>Trichothecenes - metabolism</subject><subject>Trichothecenes - toxicity</subject><subject>Triticum - microbiology</subject><subject>Yeast cell toxicity test</subject><issn>1056-9014</issn><issn>1522-7189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU-LFDEQxRtR1nX15E0QcvIirZU_3ek-yuCuyjKCO6vgJaSTyk50ujMmaXQ-gt_arD0snjxVFfV7D-pVVT2l8IoCsNe071sQwNcbfq86pQ1jtaRdf7_00LR1D1Q8rB6l9A0AesrZSXXSScF7zk6r36sw7nX0KUwkOGIxo8m-DCPmbbCJuBBJjt5sQ96iwQkT2cdgZ4OWDAdyPqeinkeS9iGGv6AP3haqeLhgLUaiJ0tuovZTIjoT653DiFMm2keynUdvffaYHlcPnN4lfHKsZ9X1-dvN6l19-fHi_erNZW0456JGGDrB-t5Ja6AZmk5SbctVrZadEy21YmgHaQQXjZOoBYIdXMkEqQU0g-Zn1YvFt5zxY8aU1eiTwd1OTxjmpGjTdF3btQV8uYAmhpQiOrWPftTxoCio2-DVP8EX-vnRdh5GtHfsMemyb5b9T7_Dw_-s1HrDWga3E4iiqxedTxl_3el0_K5ayWWjvqwvFL0SHz5_Wn1V68I_W3ing9I35bPq-qqXrAPg_A9nTKfC</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Nikulin, M. (University of Helsinki, Helsinki.)</creator><creator>Lappalainen, S</creator><creator>Pasanen, A.L</creator><creator>Laamanen, I</creator><creator>Veijalainen, P</creator><creator>Berg, S</creator><creator>Hintikka, E.L</creator><general>John Wiley & Sons, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>1996</creationdate><title>Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities</title><author>Nikulin, M. (University of Helsinki, Helsinki.) ; Lappalainen, S ; Pasanen, A.L ; Laamanen, I ; Veijalainen, P ; Berg, S ; Hintikka, E.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3334-e0b84299f7dc05b5871ad9136a78f461d4b6b7c4345f7ea4e0dbf152e1d0ecba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ALIMENT POUR ANIMAUX</topic><topic>Animal Feed - microbiology</topic><topic>Animals</topic><topic>Avena - microbiology</topic><topic>Carbon Dioxide - analysis</topic><topic>Cats</topic><topic>Cell Line</topic><topic>Chemical analysis</topic><topic>Cytotoxicity</topic><topic>Cytotoxins - chemistry</topic><topic>Cytotoxins - isolation & purification</topic><topic>Cytotoxins - metabolism</topic><topic>Cytotoxins - toxicity</topic><topic>Dermotoxicity</topic><topic>Dermotoxins - chemistry</topic><topic>Dermotoxins - isolation & purification</topic><topic>Dermotoxins - metabolism</topic><topic>Dermotoxins - toxicity</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Food Microbiology</topic><topic>FUSARIUM</topic><topic>Fusarium - growth & development</topic><topic>Fusarium - metabolism</topic><topic>Fusarium sporotrichioides</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>GRAIN</topic><topic>GRANOS</topic><topic>Humidity</topic><topic>Lung - cytology</topic><topic>Lung - drug effects</topic><topic>Lung - embryology</topic><topic>PIENSOS</topic><topic>Poaceae - microbiology</topic><topic>Rabbits</topic><topic>Relative humidity of air</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Skin - drug effects</topic><topic>Species Specificity</topic><topic>T-2 ELISA</topic><topic>T-2 toxin</topic><topic>TRICHOTHECENE</topic><topic>TRICHOTHECENES</topic><topic>Trichothecenes - chemistry</topic><topic>Trichothecenes - isolation & purification</topic><topic>Trichothecenes - metabolism</topic><topic>Trichothecenes - toxicity</topic><topic>Triticum - microbiology</topic><topic>Yeast cell toxicity test</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nikulin, M. (University of Helsinki, Helsinki.)</creatorcontrib><creatorcontrib>Lappalainen, S</creatorcontrib><creatorcontrib>Pasanen, A.L</creatorcontrib><creatorcontrib>Laamanen, I</creatorcontrib><creatorcontrib>Veijalainen, P</creatorcontrib><creatorcontrib>Berg, S</creatorcontrib><creatorcontrib>Hintikka, E.L</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Natural toxins</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nikulin, M. (University of Helsinki, Helsinki.)</au><au>Lappalainen, S</au><au>Pasanen, A.L</au><au>Laamanen, I</au><au>Veijalainen, P</au><au>Berg, S</au><au>Hintikka, E.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities</atitle><jtitle>Natural toxins</jtitle><addtitle>Nat. Toxins</addtitle><date>1996</date><risdate>1996</risdate><volume>4</volume><issue>3</issue><spage>117</spage><epage>121</epage><pages>117-121</pages><issn>1056-9014</issn><eissn>1522-7189</eissn><abstract>Growth and toxin production of a highly toxic strain of Fusarium sporotrichioides Sherb. were studied on oat and wheat grains and on straw under experimental conditions, in which relative humidity (RH) of air was regulated. The materials were incubated at three different RH levels at a range of 84-100%. F. sporotrichioides grew well on oat and wheat grains at RH 97-100% but grew less well at RH 84-88% and on straw. Toxin production was measured with three biological toxicity tests (cytotoxicity test, dermotoxicity test, and yeast cell toxicity test), with chemical analysis, and T-2 ELISA assay. Cytotoxicity and production of trichothecene mycotoxins were detected in all the samples incubated at all three RH levels. On oat and wheat grains, T-2 toxin, neosolaniol, and diacetoxyscirpenol were found, and on straw T-2 toxin, HT-2 toxin, neosolaniol, and T-2 tetraol were determined. In the T-2 ELISA assay, all material samples were found to contain T-2 toxin. The cytotoxicity test was the most sensitive method for detecting biological toxicity of samples inoculated with fungus. The T-2 ELISA assay and chemical analysis were about equally sensitive to detect T-2 toxin in samples</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>8743932</pmid><doi>10.1002/19960403NT3</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1056-9014 |
| ispartof | Natural toxins, 1996, Vol.4 (3), p.117-121 |
| issn | 1056-9014 1522-7189 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15588686 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | ALIMENT POUR ANIMAUX Animal Feed - microbiology Animals Avena - microbiology Carbon Dioxide - analysis Cats Cell Line Chemical analysis Cytotoxicity Cytotoxins - chemistry Cytotoxins - isolation & purification Cytotoxins - metabolism Cytotoxins - toxicity Dermotoxicity Dermotoxins - chemistry Dermotoxins - isolation & purification Dermotoxins - metabolism Dermotoxins - toxicity Enzyme-Linked Immunosorbent Assay Food Microbiology FUSARIUM Fusarium - growth & development Fusarium - metabolism Fusarium sporotrichioides Gas Chromatography-Mass Spectrometry GRAIN GRANOS Humidity Lung - cytology Lung - drug effects Lung - embryology PIENSOS Poaceae - microbiology Rabbits Relative humidity of air Saccharomyces cerevisiae - drug effects Skin - drug effects Species Specificity T-2 ELISA T-2 toxin TRICHOTHECENE TRICHOTHECENES Trichothecenes - chemistry Trichothecenes - isolation & purification Trichothecenes - metabolism Trichothecenes - toxicity Triticum - microbiology Yeast cell toxicity test |
| title | Comparison of detection methods for trichothecenes produced by Fusarium sporotrichioides on fodder and grains at different air humidities |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T20%3A46%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20detection%20methods%20for%20trichothecenes%20produced%20by%20Fusarium%20sporotrichioides%20on%20fodder%20and%20grains%20at%20different%20air%20humidities&rft.jtitle=Natural%20toxins&rft.au=Nikulin,%20M.%20(University%20of%20Helsinki,%20Helsinki.)&rft.date=1996&rft.volume=4&rft.issue=3&rft.spage=117&rft.epage=121&rft.pages=117-121&rft.issn=1056-9014&rft.eissn=1522-7189&rft_id=info:doi/10.1002/19960403NT3&rft_dat=%3Cproquest_cross%3E15588686%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15588686&rft_id=info:pmid/8743932&rfr_iscdi=true |