Alcohol-FET sensor based on a complex cell membrane enzyme system
An alcohol -FET sensor was developed by use of a complex enzyme system in a cell membrane and an ion-sensitive field effect transistor (ISFET). The cell membrane of Gluconobacter suboxydans IFO 12528, which converts ethanol to acetic acid, was immobilized on the gate of an ISFET with calcium alginat...
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| Veröffentlicht in: | Analytica chimica acta 1988, Vol.207 (1-2), p.77-84 |
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| container_issue | 1-2 |
| container_start_page | 77 |
| container_title | Analytica chimica acta |
| container_volume | 207 |
| creator | Tamiya, Eiichi Karube, Isao Kitagawa, Yasushi Ameyama, Minoru Nakashima, Koji |
| description | An alcohol -FET sensor was developed by use of a complex enzyme system in a cell membrane and an ion-sensitive field effect transistor (ISFET). The cell membrane of
Gluconobacter suboxydans IFO 12528, which converts ethanol to acetic acid, was immobilized on the gate of an ISFET with calcium alginate gel coated with nitrocellulose. This ISFET (1), a reference ISFET without the cell membrane (ISFET 2) and an Ag/AgCl reference electrode were placed in 5 mM Trismalate buffer (pH 5.5, 25°C), and the differential output between ISFETS 1 and 2 was measured. The output of the sensor was stabilized by adding pyrroloquinoline quinone. The response time was ca. 10 min., and there was a linear relationship between the differential output voltage and the ethanol concentration up to 20 mg l
−1. The output of the sensor was stable for 40 h below 30°C. The sensor responded to ethanol, propan- 1-ol and butan- 1-ol, but not to methanol, propan-2-ol and butan-2-ol. The sensor was used to determine blood ethanol. |
| doi_str_mv | 10.1016/S0003-2670(00)80784-9 |
| format | Article |
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−1. The output of the sensor was stable for 40 h below 30°C. The sensor responded to ethanol, propan- 1-ol and butan- 1-ol, but not to methanol, propan-2-ol and butan-2-ol. The sensor was used to determine blood ethanol.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/S0003-2670(00)80784-9</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>acetic acid ; Alcoholism and acute alcohol poisoning ; alcohols ; Biological and medical sciences ; Biotechnology ; blood ; cell membranes ; enzymes ; ethanol ; Fundamental and applied biological sciences. Psychology ; Gluconobacter suboxydans ; Immobilization of organelles and whole cells ; Immobilization techniques ; Medical sciences ; Methods. Procedures. Technologies ; sensors ; Toxicology</subject><ispartof>Analytica chimica acta, 1988, Vol.207 (1-2), p.77-84</ispartof><rights>1988 Elsevier Science Publishers B.V.</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-6e99b5c91dddc1d47812afc5395572955dfeb2f3127664600c54d1d1e937e1153</citedby><cites>FETCH-LOGICAL-c469t-6e99b5c91dddc1d47812afc5395572955dfeb2f3127664600c54d1d1e937e1153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003267000807849$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7157214$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamiya, Eiichi</creatorcontrib><creatorcontrib>Karube, Isao</creatorcontrib><creatorcontrib>Kitagawa, Yasushi</creatorcontrib><creatorcontrib>Ameyama, Minoru</creatorcontrib><creatorcontrib>Nakashima, Koji</creatorcontrib><title>Alcohol-FET sensor based on a complex cell membrane enzyme system</title><title>Analytica chimica acta</title><description>An alcohol -FET sensor was developed by use of a complex enzyme system in a cell membrane and an ion-sensitive field effect transistor (ISFET). The cell membrane of
Gluconobacter suboxydans IFO 12528, which converts ethanol to acetic acid, was immobilized on the gate of an ISFET with calcium alginate gel coated with nitrocellulose. This ISFET (1), a reference ISFET without the cell membrane (ISFET 2) and an Ag/AgCl reference electrode were placed in 5 mM Trismalate buffer (pH 5.5, 25°C), and the differential output between ISFETS 1 and 2 was measured. The output of the sensor was stabilized by adding pyrroloquinoline quinone. The response time was ca. 10 min., and there was a linear relationship between the differential output voltage and the ethanol concentration up to 20 mg l
−1. The output of the sensor was stable for 40 h below 30°C. The sensor responded to ethanol, propan- 1-ol and butan- 1-ol, but not to methanol, propan-2-ol and butan-2-ol. The sensor was used to determine blood ethanol.</description><subject>acetic acid</subject><subject>Alcoholism and acute alcohol poisoning</subject><subject>alcohols</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>blood</subject><subject>cell membranes</subject><subject>enzymes</subject><subject>ethanol</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gluconobacter suboxydans</subject><subject>Immobilization of organelles and whole cells</subject><subject>Immobilization techniques</subject><subject>Medical sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>sensors</subject><subject>Toxicology</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAQhoMouK7-BCEHET1UM23SNCdZFr9gwYN6DtlkipW2WZOuuP56sx_s1csMA8-8885LyDmwG2BQ3r4yxoosLyW7Yuy6YrLimTogI6hkkfEi54dktEeOyUmMn2nMgfERmUxa6z98mz3cv9GIffSBzk1ER31PDbW-W7T4Qy22Le2wmwfTI8X-d9Uhjas4YHdKjmrTRjzb9TF5T1rTp2z28vg8ncwyy0s1ZCUqNRdWgXPOguOygtzUVhRKCJmn4mqc53UBuSxLXjJmBXfgAFUhEUAUY3K51V0E_7XEOOiuiWtfyZFfRg1CVCKpJlBsQRt8jAFrvQhNZ8JKA9PrwPQmML1OQzOmN4FplfYudgdMtKat06u2iftlCckn8ITdbTFMz343GHS0DfYWXRPQDtr55p9Df2m1fZg</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Tamiya, Eiichi</creator><creator>Karube, Isao</creator><creator>Kitagawa, Yasushi</creator><creator>Ameyama, Minoru</creator><creator>Nakashima, Koji</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1988</creationdate><title>Alcohol-FET sensor based on a complex cell membrane enzyme system</title><author>Tamiya, Eiichi ; Karube, Isao ; Kitagawa, Yasushi ; Ameyama, Minoru ; Nakashima, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-6e99b5c91dddc1d47812afc5395572955dfeb2f3127664600c54d1d1e937e1153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>acetic acid</topic><topic>Alcoholism and acute alcohol poisoning</topic><topic>alcohols</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>blood</topic><topic>cell membranes</topic><topic>enzymes</topic><topic>ethanol</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gluconobacter suboxydans</topic><topic>Immobilization of organelles and whole cells</topic><topic>Immobilization techniques</topic><topic>Medical sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>sensors</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamiya, Eiichi</creatorcontrib><creatorcontrib>Karube, Isao</creatorcontrib><creatorcontrib>Kitagawa, Yasushi</creatorcontrib><creatorcontrib>Ameyama, Minoru</creatorcontrib><creatorcontrib>Nakashima, Koji</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamiya, Eiichi</au><au>Karube, Isao</au><au>Kitagawa, Yasushi</au><au>Ameyama, Minoru</au><au>Nakashima, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alcohol-FET sensor based on a complex cell membrane enzyme system</atitle><jtitle>Analytica chimica acta</jtitle><date>1988</date><risdate>1988</risdate><volume>207</volume><issue>1-2</issue><spage>77</spage><epage>84</epage><pages>77-84</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>An alcohol -FET sensor was developed by use of a complex enzyme system in a cell membrane and an ion-sensitive field effect transistor (ISFET). The cell membrane of
Gluconobacter suboxydans IFO 12528, which converts ethanol to acetic acid, was immobilized on the gate of an ISFET with calcium alginate gel coated with nitrocellulose. This ISFET (1), a reference ISFET without the cell membrane (ISFET 2) and an Ag/AgCl reference electrode were placed in 5 mM Trismalate buffer (pH 5.5, 25°C), and the differential output between ISFETS 1 and 2 was measured. The output of the sensor was stabilized by adding pyrroloquinoline quinone. The response time was ca. 10 min., and there was a linear relationship between the differential output voltage and the ethanol concentration up to 20 mg l
−1. The output of the sensor was stable for 40 h below 30°C. The sensor responded to ethanol, propan- 1-ol and butan- 1-ol, but not to methanol, propan-2-ol and butan-2-ol. The sensor was used to determine blood ethanol.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0003-2670(00)80784-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytica chimica acta, 1988, Vol.207 (1-2), p.77-84 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | acetic acid Alcoholism and acute alcohol poisoning alcohols Biological and medical sciences Biotechnology blood cell membranes enzymes ethanol Fundamental and applied biological sciences. Psychology Gluconobacter suboxydans Immobilization of organelles and whole cells Immobilization techniques Medical sciences Methods. Procedures. Technologies sensors Toxicology |
| title | Alcohol-FET sensor based on a complex cell membrane enzyme system |
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