Complete Enzymic Dephosphorylation of Corn-Soybean Meal Feed under Simulated Intestinal Conditions of the Turkey
A crude laboratory phytase preparation, a commercial phytase, and a commercial experimental phytase were used in vitro to study the extent of corn-soybean feed (P sub(tot): 4.83 plus or minus 0.06 g/kg) dephosphorylation under simulated intestinal conditions of the turkey. The phytases had different...
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Veröffentlicht in: | Journal of agricultural and food chemistry 1995-02, Vol.43 (2), p.288-294 |
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creator | Zyla, Krzysztof Ledoux, David R Veum, Trygve L |
description | A crude laboratory phytase preparation, a commercial phytase, and a commercial experimental phytase were used in vitro to study the extent of corn-soybean feed (P sub(tot): 4.83 plus or minus 0.06 g/kg) dephosphorylation under simulated intestinal conditions of the turkey. The phytases had different specific activities; varied in side activities of acid protease, acid phosphatase, and pectinase; and revealed different abilities for feed dephosphorylation. Acid phosphatase was shown to be a key activity in feed dephosphorylation, while fungal acid protease enhanced dephosphorylation by stimulating gastric digestion. An "enzymic cocktail" that completely dephosphorylated phytates present in corn-soybean feed also contained pectinase and citric acid. Complete dephosphorylation was accompanied by 12-29% increase in dialyzable protein, and 45-81% increase in the concentration of reducing sugars liberated from feed. The potential use of the "cocktail" as a feed additive for monogastric animal may result in improved digestibility of phytate phosphorus, protein, and carbohydrates. |
doi_str_mv | 10.1021/jf00050a005 |
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The phytases had different specific activities; varied in side activities of acid protease, acid phosphatase, and pectinase; and revealed different abilities for feed dephosphorylation. Acid phosphatase was shown to be a key activity in feed dephosphorylation, while fungal acid protease enhanced dephosphorylation by stimulating gastric digestion. An "enzymic cocktail" that completely dephosphorylated phytates present in corn-soybean feed also contained pectinase and citric acid. Complete dephosphorylation was accompanied by 12-29% increase in dialyzable protein, and 45-81% increase in the concentration of reducing sugars liberated from feed. 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Agric. Food Chem</addtitle><description>A crude laboratory phytase preparation, a commercial phytase, and a commercial experimental phytase were used in vitro to study the extent of corn-soybean feed (P sub(tot): 4.83 plus or minus 0.06 g/kg) dephosphorylation under simulated intestinal conditions of the turkey. The phytases had different specific activities; varied in side activities of acid protease, acid phosphatase, and pectinase; and revealed different abilities for feed dephosphorylation. Acid phosphatase was shown to be a key activity in feed dephosphorylation, while fungal acid protease enhanced dephosphorylation by stimulating gastric digestion. An "enzymic cocktail" that completely dephosphorylated phytates present in corn-soybean feed also contained pectinase and citric acid. Complete dephosphorylation was accompanied by 12-29% increase in dialyzable protein, and 45-81% increase in the concentration of reducing sugars liberated from feed. 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Complete dephosphorylation was accompanied by 12-29% increase in dialyzable protein, and 45-81% increase in the concentration of reducing sugars liberated from feed. The potential use of the "cocktail" as a feed additive for monogastric animal may result in improved digestibility of phytate phosphorus, protein, and carbohydrates.</abstract><pub>American Chemical Society</pub><doi>10.1021/jf00050a005</doi><tpages>7</tpages></addata></record> |
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title | Complete Enzymic Dephosphorylation of Corn-Soybean Meal Feed under Simulated Intestinal Conditions of the Turkey |
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