High incidence of false positives by a latex agglutination test for the diagnosis of Clostridium difficile associated colitis in compromised patients
Detection of Clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitics has been used for over a decade. Because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (LA) kit has attracted much atten...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 1989-07, Vol.12 (4), p.291-294 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Hussain Qadri, S.M. Akhter, Javed Ostrawski, S. Qadri, S.G.M. Cunha, Burke A. |
| description | Detection of
Clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitics has been used for over a decade. Because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (LA) kit has attracted much attention. We compared the sensitivity and specificity of this method with the cytotoxic assay (CTA) using diarrheal stools from 652 patients at a referral tertiary care center. Specimens from 71 (10.9%) patients were found positive with CTA and 98 (15%) by LA. Of these, 67 stools were positive by both methods. Four specimens showed cytotoxicity but were negative by LA. Of the 31 patient specimens that were positive by LA but negative by CTA, 23 were obtained from leukemic, bone marrow transplant and four from renal transplant patients. Thirteen of these patients had
Giardia lamblia (three),
Salmonella enteritidis (three),
Blastocystis hominis (four),
Rotavirus (two), and
Shigella boydii (one) in their stools. No significant organisms were found in the rest of the LA-positive and CTA-negative specimens. |
| doi_str_mv | 10.1016/0732-8893(89)90092-8 |
| format | Article |
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Clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitics has been used for over a decade. Because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (LA) kit has attracted much attention. We compared the sensitivity and specificity of this method with the cytotoxic assay (CTA) using diarrheal stools from 652 patients at a referral tertiary care center. Specimens from 71 (10.9%) patients were found positive with CTA and 98 (15%) by LA. Of these, 67 stools were positive by both methods. Four specimens showed cytotoxicity but were negative by LA. Of the 31 patient specimens that were positive by LA but negative by CTA, 23 were obtained from leukemic, bone marrow transplant and four from renal transplant patients. Thirteen of these patients had
Giardia lamblia (three),
Salmonella enteritidis (three),
Blastocystis hominis (four),
Rotavirus (two), and
Shigella boydii (one) in their stools. No significant organisms were found in the rest of the LA-positive and CTA-negative specimens.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/0732-8893(89)90092-8</identifier><identifier>PMID: 2591165</identifier><identifier>CODEN: DMIDDZ</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Bone Marrow Transplantation ; Cytotoxicity Tests, Immunologic ; Enterocolitis, Pseudomembranous - complications ; Enterocolitis, Pseudomembranous - diagnosis ; Evaluation Studies as Topic ; False Positive Reactions ; Human bacterial diseases ; Humans ; Infectious diseases ; Kidney Transplantation ; Latex Fixation Tests - methods ; Leukemia - complications ; Medical sciences</subject><ispartof>Diagnostic microbiology and infectious disease, 1989-07, Vol.12 (4), p.291-294</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-4141b8e43974a3ae6a83f07441a3d28b44a33c14f7c067583788246e540751b93</citedby><cites>FETCH-LOGICAL-c417t-4141b8e43974a3ae6a83f07441a3d28b44a33c14f7c067583788246e540751b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0732889389900928$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27902,27903,65308</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6748768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2591165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hussain Qadri, S.M.</creatorcontrib><creatorcontrib>Akhter, Javed</creatorcontrib><creatorcontrib>Ostrawski, S.</creatorcontrib><creatorcontrib>Qadri, S.G.M.</creatorcontrib><creatorcontrib>Cunha, Burke A.</creatorcontrib><title>High incidence of false positives by a latex agglutination test for the diagnosis of Clostridium difficile associated colitis in compromised patients</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>Detection of
Clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitics has been used for over a decade. Because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (LA) kit has attracted much attention. We compared the sensitivity and specificity of this method with the cytotoxic assay (CTA) using diarrheal stools from 652 patients at a referral tertiary care center. Specimens from 71 (10.9%) patients were found positive with CTA and 98 (15%) by LA. Of these, 67 stools were positive by both methods. Four specimens showed cytotoxicity but were negative by LA. Of the 31 patient specimens that were positive by LA but negative by CTA, 23 were obtained from leukemic, bone marrow transplant and four from renal transplant patients. Thirteen of these patients had
Giardia lamblia (three),
Salmonella enteritidis (three),
Blastocystis hominis (four),
Rotavirus (two), and
Shigella boydii (one) in their stools. No significant organisms were found in the rest of the LA-positive and CTA-negative specimens.</description><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Transplantation</subject><subject>Cytotoxicity Tests, Immunologic</subject><subject>Enterocolitis, Pseudomembranous - complications</subject><subject>Enterocolitis, Pseudomembranous - diagnosis</subject><subject>Evaluation Studies as Topic</subject><subject>False Positive Reactions</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Kidney Transplantation</subject><subject>Latex Fixation Tests - methods</subject><subject>Leukemia - complications</subject><subject>Medical sciences</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcFu1DAQtRCobAt_AJIPCMEhxY6d2L4goRWllSpxKWfLcSZboyRePE5FP6T_i9Nd7ZGT7Zk3b57fI-QdZ5ec8fYLU6KutDbikzafDWOmvF6QDdfKVIwp9pJsTpDX5BzxN2O8NpKdkbO6MZy3zYY8XYfdPQ2zDz3MHmgc6OBGBLqPGHJ4AKTdI3V0dBn-UrfbjUsOs8shzjQDZjrERPM90D643VxmcKXYjhFzCn1YptIYhuDDCNQhRh8KUU99HAs7lsXlOu1TnAKW8r4Qw5zxDXn1rOLt8bwgv66-322vq9ufP262324rL7nKleSSdxqkMEo64aB1WgxMScmd6GvdyVIVnstBedaqRguldS1baCRTDe-MuCAfD7xFwZ-lfMcWHR7G0c0QF7S8aVQrNCtAeQD6FBETDHafwuTSo-XMrmnY1Wq7Wm21sc9pWF3G3h_5l26C_jR0tL_0Pxz7Dr0bh-RKEHiCtUpq1a40Xw8wKF48BEgWfVjj6kMCn20fw_91_AMUwafs</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Hussain Qadri, S.M.</creator><creator>Akhter, Javed</creator><creator>Ostrawski, S.</creator><creator>Qadri, S.G.M.</creator><creator>Cunha, Burke A.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19890701</creationdate><title>High incidence of false positives by a latex agglutination test for the diagnosis of Clostridium difficile associated colitis in compromised patients</title><author>Hussain Qadri, S.M. ; Akhter, Javed ; Ostrawski, S. ; Qadri, S.G.M. ; Cunha, Burke A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-4141b8e43974a3ae6a83f07441a3d28b44a33c14f7c067583788246e540751b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Transplantation</topic><topic>Cytotoxicity Tests, Immunologic</topic><topic>Enterocolitis, Pseudomembranous - complications</topic><topic>Enterocolitis, Pseudomembranous - diagnosis</topic><topic>Evaluation Studies as Topic</topic><topic>False Positive Reactions</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Kidney Transplantation</topic><topic>Latex Fixation Tests - methods</topic><topic>Leukemia - complications</topic><topic>Medical sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hussain Qadri, S.M.</creatorcontrib><creatorcontrib>Akhter, Javed</creatorcontrib><creatorcontrib>Ostrawski, S.</creatorcontrib><creatorcontrib>Qadri, S.G.M.</creatorcontrib><creatorcontrib>Cunha, Burke A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hussain Qadri, S.M.</au><au>Akhter, Javed</au><au>Ostrawski, S.</au><au>Qadri, S.G.M.</au><au>Cunha, Burke A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High incidence of false positives by a latex agglutination test for the diagnosis of Clostridium difficile associated colitis in compromised patients</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>12</volume><issue>4</issue><spage>291</spage><epage>294</epage><pages>291-294</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><coden>DMIDDZ</coden><abstract>Detection of
Clostridium difficile cytotoxin using cell culture assays for the diagnosis of antibiotic-associated colitics has been used for over a decade. Because the methodology is time consuming and cumbersome, a recently introduced commercial latex agglutination (LA) kit has attracted much attention. We compared the sensitivity and specificity of this method with the cytotoxic assay (CTA) using diarrheal stools from 652 patients at a referral tertiary care center. Specimens from 71 (10.9%) patients were found positive with CTA and 98 (15%) by LA. Of these, 67 stools were positive by both methods. Four specimens showed cytotoxicity but were negative by LA. Of the 31 patient specimens that were positive by LA but negative by CTA, 23 were obtained from leukemic, bone marrow transplant and four from renal transplant patients. Thirteen of these patients had
Giardia lamblia (three),
Salmonella enteritidis (three),
Blastocystis hominis (four),
Rotavirus (two), and
Shigella boydii (one) in their stools. No significant organisms were found in the rest of the LA-positive and CTA-negative specimens.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2591165</pmid><doi>10.1016/0732-8893(89)90092-8</doi><tpages>4</tpages></addata></record> |
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| issn | 0732-8893 1879-0070 |
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| subjects | Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Bone Marrow Transplantation Cytotoxicity Tests, Immunologic Enterocolitis, Pseudomembranous - complications Enterocolitis, Pseudomembranous - diagnosis Evaluation Studies as Topic False Positive Reactions Human bacterial diseases Humans Infectious diseases Kidney Transplantation Latex Fixation Tests - methods Leukemia - complications Medical sciences |
| title | High incidence of false positives by a latex agglutination test for the diagnosis of Clostridium difficile associated colitis in compromised patients |
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