Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects

The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteri...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1995/11/15, Vol.43(11), pp.1831-1835
Hauptverfasser: SHIBATA, Akira, KAWANAMI, Shinobu, USHIO, Kazumichi, UENO, Satoru, YAMASHITA, Takuya
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container_end_page 1835
container_issue 11
container_start_page 1831
container_title Chemical & pharmaceutical bulletin
container_volume 43
creator SHIBATA, Akira
KAWANAMI, Shinobu
USHIO, Kazumichi
UENO, Satoru
YAMASHITA, Takuya
description The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteriorhodopsin (bR) in the purple membrane were investigated by circular dichroism (CD) and adsorbance measurements. These alcohols increased the 530-nm ellipticity of bR at low concentrations of the alcohols and decreased it at high concentrations. The addition of alcohol at a low concentration in the purple membrane aqueous suspension tended to favor the more folded-compact conformation of the bR by the enhancement of hydrophobic interaction. Alcohols at high concentrations caused the cooperative denaturation of bR due to the access of alcohol to the retinal chromophore. Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR.
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Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR.</description><subject>1-alcohol</subject><subject>anesthetic</subject><subject>Anesthetics. Neuromuscular blocking agents</subject><subject>bacteriorhodopsin</subject><subject>Biological and medical sciences</subject><subject>circular dichroism</subject><subject>Halobacterium salinarum</subject><subject>hydrophobic interaction</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Pharmacology. 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Neuromuscular blocking agents</topic><topic>bacteriorhodopsin</topic><topic>Biological and medical sciences</topic><topic>circular dichroism</topic><topic>Halobacterium salinarum</topic><topic>hydrophobic interaction</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Pharmacology. 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subjects 1-alcohol
anesthetic
Anesthetics. Neuromuscular blocking agents
bacteriorhodopsin
Biological and medical sciences
circular dichroism
Halobacterium salinarum
hydrophobic interaction
Medical sciences
Neuropharmacology
Pharmacology. Drug treatments
title Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects
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