Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects
The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteri...
Gespeichert in:
Veröffentlicht in: | Chemical & pharmaceutical bulletin 1995/11/15, Vol.43(11), pp.1831-1835 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1835 |
---|---|
container_issue | 11 |
container_start_page | 1831 |
container_title | Chemical & pharmaceutical bulletin |
container_volume | 43 |
creator | SHIBATA, Akira KAWANAMI, Shinobu USHIO, Kazumichi UENO, Satoru YAMASHITA, Takuya |
description | The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteriorhodopsin (bR) in the purple membrane were investigated by circular dichroism (CD) and adsorbance measurements. These alcohols increased the 530-nm ellipticity of bR at low concentrations of the alcohols and decreased it at high concentrations. The addition of alcohol at a low concentration in the purple membrane aqueous suspension tended to favor the more folded-compact conformation of the bR by the enhancement of hydrophobic interaction. Alcohols at high concentrations caused the cooperative denaturation of bR due to the access of alcohol to the retinal chromophore. Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR. |
doi_str_mv | 10.1248/cpb.43.1831 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15573388</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15573388</sourcerecordid><originalsourceid>FETCH-LOGICAL-c587t-b095c104ef0d596b30a358d9ebf09636dbd15d8084d5174bd5e6f1bddc60f5543</originalsourceid><addsrcrecordid>eNpFkD1PwzAQhi0EEqUw8QcyIBaUYsd24rCVqoWKSiwwW44_iKvUDnY68O9xaFWWO-nuuUenF4BbBGeoIOxR9s2M4BliGJ2BCcKkymlR4HMwgRDWeYFLfAmuYtxCWFBY4Ql4mzsdh1YPVubPQg46WB9ar3wfrcusS4M0td5lT9m8k771Xb52ai-1yp5t34poZbY0RsshXoMLI7qob459Cj5Xy4_Fa755f1kv5ptcUlYNeQNrKhEk2kBF67LBUGDKVK0bA-sSl6pRiCoGGVEUVaRRVJcGNUrJEhpKCZ6C-4O3D_57n97nOxul7jrhtN9HjiitMGYsgQ8HUAYfY9CG98HuRPjhCPIxMJ4C4wTzMbBE3x21IkrRmSCctPF0UtSEVbRI2OqAbeMgvvRpL0LKsNOjEtWU_WnRsY7-f6AVgWuHfwGg0IO6</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15573388</pqid></control><display><type>article</type><title>Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects</title><source>J-STAGE Free</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>SHIBATA, Akira ; KAWANAMI, Shinobu ; USHIO, Kazumichi ; UENO, Satoru ; YAMASHITA, Takuya</creator><creatorcontrib>SHIBATA, Akira ; KAWANAMI, Shinobu ; USHIO, Kazumichi ; UENO, Satoru ; YAMASHITA, Takuya</creatorcontrib><description>The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteriorhodopsin (bR) in the purple membrane were investigated by circular dichroism (CD) and adsorbance measurements. These alcohols increased the 530-nm ellipticity of bR at low concentrations of the alcohols and decreased it at high concentrations. The addition of alcohol at a low concentration in the purple membrane aqueous suspension tended to favor the more folded-compact conformation of the bR by the enhancement of hydrophobic interaction. Alcohols at high concentrations caused the cooperative denaturation of bR due to the access of alcohol to the retinal chromophore. Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR.</description><identifier>ISSN: 0009-2363</identifier><identifier>EISSN: 1347-5223</identifier><identifier>DOI: 10.1248/cpb.43.1831</identifier><identifier>CODEN: CPBTAL</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>1-alcohol ; anesthetic ; Anesthetics. Neuromuscular blocking agents ; bacteriorhodopsin ; Biological and medical sciences ; circular dichroism ; Halobacterium salinarum ; hydrophobic interaction ; Medical sciences ; Neuropharmacology ; Pharmacology. Drug treatments</subject><ispartof>Chemical and Pharmaceutical Bulletin, 1995/11/15, Vol.43(11), pp.1831-1835</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-b095c104ef0d596b30a358d9ebf09636dbd15d8084d5174bd5e6f1bddc60f5543</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2948752$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>SHIBATA, Akira</creatorcontrib><creatorcontrib>KAWANAMI, Shinobu</creatorcontrib><creatorcontrib>USHIO, Kazumichi</creatorcontrib><creatorcontrib>UENO, Satoru</creatorcontrib><creatorcontrib>YAMASHITA, Takuya</creatorcontrib><title>Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects</title><title>Chemical & pharmaceutical bulletin</title><addtitle>Chem. Pharm. Bull.</addtitle><description>The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteriorhodopsin (bR) in the purple membrane were investigated by circular dichroism (CD) and adsorbance measurements. These alcohols increased the 530-nm ellipticity of bR at low concentrations of the alcohols and decreased it at high concentrations. The addition of alcohol at a low concentration in the purple membrane aqueous suspension tended to favor the more folded-compact conformation of the bR by the enhancement of hydrophobic interaction. Alcohols at high concentrations caused the cooperative denaturation of bR due to the access of alcohol to the retinal chromophore. Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR.</description><subject>1-alcohol</subject><subject>anesthetic</subject><subject>Anesthetics. Neuromuscular blocking agents</subject><subject>bacteriorhodopsin</subject><subject>Biological and medical sciences</subject><subject>circular dichroism</subject><subject>Halobacterium salinarum</subject><subject>hydrophobic interaction</subject><subject>Medical sciences</subject><subject>Neuropharmacology</subject><subject>Pharmacology. Drug treatments</subject><issn>0009-2363</issn><issn>1347-5223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNpFkD1PwzAQhi0EEqUw8QcyIBaUYsd24rCVqoWKSiwwW44_iKvUDnY68O9xaFWWO-nuuUenF4BbBGeoIOxR9s2M4BliGJ2BCcKkymlR4HMwgRDWeYFLfAmuYtxCWFBY4Ql4mzsdh1YPVubPQg46WB9ar3wfrcusS4M0td5lT9m8k771Xb52ai-1yp5t34poZbY0RsshXoMLI7qob459Cj5Xy4_Fa755f1kv5ptcUlYNeQNrKhEk2kBF67LBUGDKVK0bA-sSl6pRiCoGGVEUVaRRVJcGNUrJEhpKCZ6C-4O3D_57n97nOxul7jrhtN9HjiitMGYsgQ8HUAYfY9CG98HuRPjhCPIxMJ4C4wTzMbBE3x21IkrRmSCctPF0UtSEVbRI2OqAbeMgvvRpL0LKsNOjEtWU_WnRsY7-f6AVgWuHfwGg0IO6</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>SHIBATA, Akira</creator><creator>KAWANAMI, Shinobu</creator><creator>USHIO, Kazumichi</creator><creator>UENO, Satoru</creator><creator>YAMASHITA, Takuya</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>1995</creationdate><title>Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects</title><author>SHIBATA, Akira ; KAWANAMI, Shinobu ; USHIO, Kazumichi ; UENO, Satoru ; YAMASHITA, Takuya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-b095c104ef0d596b30a358d9ebf09636dbd15d8084d5174bd5e6f1bddc60f5543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>1-alcohol</topic><topic>anesthetic</topic><topic>Anesthetics. Neuromuscular blocking agents</topic><topic>bacteriorhodopsin</topic><topic>Biological and medical sciences</topic><topic>circular dichroism</topic><topic>Halobacterium salinarum</topic><topic>hydrophobic interaction</topic><topic>Medical sciences</topic><topic>Neuropharmacology</topic><topic>Pharmacology. Drug treatments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHIBATA, Akira</creatorcontrib><creatorcontrib>KAWANAMI, Shinobu</creatorcontrib><creatorcontrib>USHIO, Kazumichi</creatorcontrib><creatorcontrib>UENO, Satoru</creatorcontrib><creatorcontrib>YAMASHITA, Takuya</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Chemical & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHIBATA, Akira</au><au>KAWANAMI, Shinobu</au><au>USHIO, Kazumichi</au><au>UENO, Satoru</au><au>YAMASHITA, Takuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects</atitle><jtitle>Chemical & pharmaceutical bulletin</jtitle><addtitle>Chem. Pharm. Bull.</addtitle><date>1995</date><risdate>1995</risdate><volume>43</volume><issue>11</issue><spage>1831</spage><epage>1835</epage><pages>1831-1835</pages><issn>0009-2363</issn><eissn>1347-5223</eissn><coden>CPBTAL</coden><abstract>The purple membrane is an appropriate model system for investigating the effects of anesthetics on the structures and functions of excitable cell membranes. The effects of anesthetically active alcohols (methanol, ethanol, 1-propanol, 1-butanol, 1-pentanol, and 1-hexanol) on the structure of bacteriorhodopsin (bR) in the purple membrane were investigated by circular dichroism (CD) and adsorbance measurements. These alcohols increased the 530-nm ellipticity of bR at low concentrations of the alcohols and decreased it at high concentrations. The addition of alcohol at a low concentration in the purple membrane aqueous suspension tended to favor the more folded-compact conformation of the bR by the enhancement of hydrophobic interaction. Alcohols at high concentrations caused the cooperative denaturation of bR due to the access of alcohol to the retinal chromophore. Our attention is mainly focused on the finding that alcohols at low concentrations induce a more folded conformation of bR.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><doi>10.1248/cpb.43.1831</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0009-2363 |
ispartof | Chemical and Pharmaceutical Bulletin, 1995/11/15, Vol.43(11), pp.1831-1835 |
issn | 0009-2363 1347-5223 |
language | eng |
recordid | cdi_proquest_miscellaneous_15573388 |
source | J-STAGE Free; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
subjects | 1-alcohol anesthetic Anesthetics. Neuromuscular blocking agents bacteriorhodopsin Biological and medical sciences circular dichroism Halobacterium salinarum hydrophobic interaction Medical sciences Neuropharmacology Pharmacology. Drug treatments |
title | Anesthetic-Bacteriorhodopsin interaction : Alcohol-Induced Biphasic Effects |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T16%3A15%3A18IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anesthetic-Bacteriorhodopsin%20interaction%20:%20Alcohol-Induced%20Biphasic%20Effects&rft.jtitle=Chemical%20&%20pharmaceutical%20bulletin&rft.au=SHIBATA,%20Akira&rft.date=1995&rft.volume=43&rft.issue=11&rft.spage=1831&rft.epage=1835&rft.pages=1831-1835&rft.issn=0009-2363&rft.eissn=1347-5223&rft.coden=CPBTAL&rft_id=info:doi/10.1248/cpb.43.1831&rft_dat=%3Cproquest_cross%3E15573388%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15573388&rft_id=info:pmid/&rfr_iscdi=true |