An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus

A single dilution enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to bovine viral diarrhea (BVD) virus in cattle sera. Viral antigen (NADL strain) was grown in a pig kidney cell line (PK15), and after removal of nuclear debris, was purified by ultracentrifugation through...

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Veröffentlicht in:	Veterinary microbiology 1990, Vol.22 (1), p.1-10
Hauptverfasser:	Durham, P.J.K., Hassard, L.E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ANTIBODIES Antibodies, Viral - analysis ANTICORPS ANTICUERPOS Antigens, Viral - biosynthesis Antigens, Viral - immunology Biological and medical sciences BOVIN CATTLE Cell Line DIARREA Diarrhea Viruses, Bovine Viral - immunology DIARRHEE DIARRHOEA ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology GANADO BOVINO Humans Infant, Newborn Microbiology Neutralization Tests Pestivirus - immunology Predictive Value of Tests Reproducibility of Results Techniques used in virology TEST ELISA Virology VIROSE VIROSES VIROSIS
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