An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus
A single dilution enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to bovine viral diarrhea (BVD) virus in cattle sera. Viral antigen (NADL strain) was grown in a pig kidney cell line (PK15), and after removal of nuclear debris, was purified by ultracentrifugation through...
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| Veröffentlicht in: | Veterinary microbiology 1990, Vol.22 (1), p.1-10 |
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| creator | Durham, P.J.K. Hassard, L.E. |
| description | A single dilution enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to bovine viral diarrhea (BVD) virus in cattle sera. Viral antigen (NADL strain) was grown in a pig kidney cell line (PK15), and after removal of nuclear debris, was purified by ultracentrifugation through a potassium tartrate cushion. Antigen grown in embryonic bovine tracheal epithelial cells was also satisfactory. The test used a high salt buffer to minimize nonspecific reactivity, polyethylene glycol to enhance the reaction, and Protein G as the labelling agent.
Comparative testing with the virus neutralization test (VNT) showed the ELISA results to have a high level of correlation with the VNT titers (
r = 0.83). In vaccinated animals the ELISA detected antibodies earlier than the VNT. All animals sampled from a BVD-free herd were negative for BVD antibody. The single dilution test showed close agreement (
r = 0.84) with ELISA values obtained using a serial dilution technique, and also proved to have a high level of reproducibility. The test procedures were relatively easy to carry out, and were economic in their use of materials. |
| doi_str_mv | 10.1016/0378-1135(90)90118-F |
| format | Article |
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Comparative testing with the virus neutralization test (VNT) showed the ELISA results to have a high level of correlation with the VNT titers (
r = 0.83). In vaccinated animals the ELISA detected antibodies earlier than the VNT. All animals sampled from a BVD-free herd were negative for BVD antibody. The single dilution test showed close agreement (
r = 0.84) with ELISA values obtained using a serial dilution technique, and also proved to have a high level of reproducibility. The test procedures were relatively easy to carry out, and were economic in their use of materials.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(90)90118-F</identifier><identifier>PMID: 2159672</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; ANTIBODIES ; Antibodies, Viral - analysis ; ANTICORPS ; ANTICUERPOS ; Antigens, Viral - biosynthesis ; Antigens, Viral - immunology ; Biological and medical sciences ; BOVIN ; CATTLE ; Cell Line ; DIARREA ; Diarrhea Viruses, Bovine Viral - immunology ; DIARRHEE ; DIARRHOEA ; ELISA ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; GANADO BOVINO ; Humans ; Infant, Newborn ; Microbiology ; Neutralization Tests ; Pestivirus - immunology ; Predictive Value of Tests ; Reproducibility of Results ; Techniques used in virology ; TEST ELISA ; Virology ; VIROSE ; VIROSES ; VIROSIS</subject><ispartof>Veterinary microbiology, 1990, Vol.22 (1), p.1-10</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-6d6b2d2cea4a911a22eb0a9a20bca71d1bd7a291bfdac3e646b634cdb90d70eb3</citedby><cites>FETCH-LOGICAL-c406t-6d6b2d2cea4a911a22eb0a9a20bca71d1bd7a291bfdac3e646b634cdb90d70eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1135(90)90118-F$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19343479$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2159672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Durham, P.J.K.</creatorcontrib><creatorcontrib>Hassard, L.E.</creatorcontrib><title>An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>A single dilution enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to bovine viral diarrhea (BVD) virus in cattle sera. Viral antigen (NADL strain) was grown in a pig kidney cell line (PK15), and after removal of nuclear debris, was purified by ultracentrifugation through a potassium tartrate cushion. Antigen grown in embryonic bovine tracheal epithelial cells was also satisfactory. The test used a high salt buffer to minimize nonspecific reactivity, polyethylene glycol to enhance the reaction, and Protein G as the labelling agent.
Comparative testing with the virus neutralization test (VNT) showed the ELISA results to have a high level of correlation with the VNT titers (
r = 0.83). In vaccinated animals the ELISA detected antibodies earlier than the VNT. All animals sampled from a BVD-free herd were negative for BVD antibody. The single dilution test showed close agreement (
r = 0.84) with ELISA values obtained using a serial dilution technique, and also proved to have a high level of reproducibility. The test procedures were relatively easy to carry out, and were economic in their use of materials.</description><subject>Animals</subject><subject>ANTIBODIES</subject><subject>Antibodies, Viral - analysis</subject><subject>ANTICORPS</subject><subject>ANTICUERPOS</subject><subject>Antigens, Viral - biosynthesis</subject><subject>Antigens, Viral - immunology</subject><subject>Biological and medical sciences</subject><subject>BOVIN</subject><subject>CATTLE</subject><subject>Cell Line</subject><subject>DIARREA</subject><subject>Diarrhea Viruses, Bovine Viral - immunology</subject><subject>DIARRHEE</subject><subject>DIARRHOEA</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANADO BOVINO</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Microbiology</subject><subject>Neutralization Tests</subject><subject>Pestivirus - immunology</subject><subject>Predictive Value of Tests</subject><subject>Reproducibility of Results</subject><subject>Techniques used in virology</subject><subject>TEST ELISA</subject><subject>Virology</subject><subject>VIROSE</subject><subject>VIROSES</subject><subject>VIROSIS</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGKFDEQhoMo67j6AqKQi7J7aK2ku9OTizAsO7ow6EG9eAmVpFqj3clu0jMwPr09zrDePBXF_9VP8TH2QsAbAUK9hbpbVkLU7YWGSw1CLKv1A7YQy66uZNvIh2xxjzxmT0r5CQCNVnDGzqRoterkgn1bRU7x936kagjxF3kexnEbU0nZUpw4loJ7fnG9ufm8uuR9yhzjFGzygQqfErdpFyLxXcg4cB8w5x-Eh3VbnrJHPQ6Fnp3mOfu6vv5y9aHafHp_c7XaVK4BNVXKKyu9dIQNaiFQSrKAGiVYh53wwvoOpRa29-hqUo2yqm6ctxp8B2Trc_b62Hub092WymTGUBwNA0ZK22JE23aihW4GmyPociolU29ucxgx740Ac1BqDr7MwZfRYP4qNev57OWpf2tH8vdHJ4dz_uqUY3E49BmjC-Vft66buun0zD0_cj0mg9_zzHzcaAChl2oO3x1Dmk3tAmVTXKDoyIdMbjI-hf9_-Qfslpt2</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Durham, P.J.K.</creator><creator>Hassard, L.E.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>1990</creationdate><title>An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus</title><author>Durham, P.J.K. ; Hassard, L.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-6d6b2d2cea4a911a22eb0a9a20bca71d1bd7a291bfdac3e646b634cdb90d70eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>ANTIBODIES</topic><topic>Antibodies, Viral - analysis</topic><topic>ANTICORPS</topic><topic>ANTICUERPOS</topic><topic>Antigens, Viral - biosynthesis</topic><topic>Antigens, Viral - immunology</topic><topic>Biological and medical sciences</topic><topic>BOVIN</topic><topic>CATTLE</topic><topic>Cell Line</topic><topic>DIARREA</topic><topic>Diarrhea Viruses, Bovine Viral - immunology</topic><topic>DIARRHEE</topic><topic>DIARRHOEA</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANADO BOVINO</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Microbiology</topic><topic>Neutralization Tests</topic><topic>Pestivirus - immunology</topic><topic>Predictive Value of Tests</topic><topic>Reproducibility of Results</topic><topic>Techniques used in virology</topic><topic>TEST ELISA</topic><topic>Virology</topic><topic>VIROSE</topic><topic>VIROSES</topic><topic>VIROSIS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Durham, P.J.K.</creatorcontrib><creatorcontrib>Hassard, L.E.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Durham, P.J.K.</au><au>Hassard, L.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1990</date><risdate>1990</risdate><volume>22</volume><issue>1</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>A single dilution enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to bovine viral diarrhea (BVD) virus in cattle sera. Viral antigen (NADL strain) was grown in a pig kidney cell line (PK15), and after removal of nuclear debris, was purified by ultracentrifugation through a potassium tartrate cushion. Antigen grown in embryonic bovine tracheal epithelial cells was also satisfactory. The test used a high salt buffer to minimize nonspecific reactivity, polyethylene glycol to enhance the reaction, and Protein G as the labelling agent.
Comparative testing with the virus neutralization test (VNT) showed the ELISA results to have a high level of correlation with the VNT titers (
r = 0.83). In vaccinated animals the ELISA detected antibodies earlier than the VNT. All animals sampled from a BVD-free herd were negative for BVD antibody. The single dilution test showed close agreement (
r = 0.84) with ELISA values obtained using a serial dilution technique, and also proved to have a high level of reproducibility. The test procedures were relatively easy to carry out, and were economic in their use of materials.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>2159672</pmid><doi>10.1016/0378-1135(90)90118-F</doi><tpages>10</tpages></addata></record> |
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| issn | 0378-1135 1873-2542 |
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| subjects | Animals ANTIBODIES Antibodies, Viral - analysis ANTICORPS ANTICUERPOS Antigens, Viral - biosynthesis Antigens, Viral - immunology Biological and medical sciences BOVIN CATTLE Cell Line DIARREA Diarrhea Viruses, Bovine Viral - immunology DIARRHEE DIARRHOEA ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology GANADO BOVINO Humans Infant, Newborn Microbiology Neutralization Tests Pestivirus - immunology Predictive Value of Tests Reproducibility of Results Techniques used in virology TEST ELISA Virology VIROSE VIROSES VIROSIS |
| title | An enzyme-linked immunosorbent assay (ELISA) for antibodies to bovine viral diarrhea virus |
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