NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleosides in yeast extracts
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H, 31P‐NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31P‐NMR resonances in the region 20–21 ppm, originating from the...
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| Veröffentlicht in: | FEMS microbiology letters 1990-03, Vol.68 (1/2), p.217-222 |
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| container_title | FEMS microbiology letters |
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| creator | Rayner, M.H Sadler, P.J Scawen, M.D |
| description | The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H, 31P‐NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. 31P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA. |
| doi_str_mv | 10.1111/j.1574-6968.1990.tb04152.x |
| format | Article |
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An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. 31P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1990.tb04152.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>31P‐NMR ; Biological and medical sciences ; culture media ; Cyclic nucleosides ; extracts ; Fundamental and applied biological sciences. 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An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. 31P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.</description><subject>31P‐NMR</subject><subject>Biological and medical sciences</subject><subject>culture media</subject><subject>Cyclic nucleosides</subject><subject>extracts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>LB broth</subject><subject>Microbiology</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>nucleosides</subject><subject>Yeast autolysis</subject><subject>Yeast extracts</subject><subject>yeasts</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqVkF1rFDEUhkOp0LX6GxoKil7MeDL5mvSioMWqsFVQex2ymRPNMrvTJjO4--_NsIteN5DkIm-el_MQcsmgZmW9W9dMalEpo9qaGQP1uALBZFPvTsji39MpWQDXbcXA6DPyPOc1AIgG1ILYr3ffaR6nLmKmQ6COrpwfMUXXU499OaZ-nBLSDXZx2tA3yw90lYbx99sr6ve-j55uJ9_jkGNXCHFL9-jySHE3pgLKL8iz4PqML4_3Obm__fjz5nO1_Pbpy837ZeV5K3UVnA8NFxK9A-TcAPNKiTYYpkXZgSuGQpqulU3HXJnJtx61bAU3DXfC8HPy-sB9SMPjhHm0m5jnAdwWhylbJqVqQfISvDoEfRpyThjsQ4obl_aWgZ2d2rWdxdlZnJ2d2qNTuyufXx1bXPauD8ltfcz_CUYAaK1K7vqQ-xN73D-hwd7eLRumC-DiAAhusO5XKiX3PxpgHBplNNOM_wXb3JO_</recordid><startdate>199003</startdate><enddate>199003</enddate><creator>Rayner, M.H</creator><creator>Sadler, P.J</creator><creator>Scawen, M.D</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>199003</creationdate><title>NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleosides in yeast extracts</title><author>Rayner, M.H ; Sadler, P.J ; Scawen, M.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3857-facf2345eca0e33901c6648f9174917f361e459d852d1a696c8ce75843923a493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>31P‐NMR</topic><topic>Biological and medical sciences</topic><topic>culture media</topic><topic>Cyclic nucleosides</topic><topic>extracts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>LB broth</topic><topic>Microbiology</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>nucleosides</topic><topic>Yeast autolysis</topic><topic>Yeast extracts</topic><topic>yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rayner, M.H</creatorcontrib><creatorcontrib>Sadler, P.J</creatorcontrib><creatorcontrib>Scawen, M.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rayner, M.H</au><au>Sadler, P.J</au><au>Scawen, M.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleosides in yeast extracts</atitle><jtitle>FEMS microbiology letters</jtitle><date>1990-03</date><risdate>1990</risdate><volume>68</volume><issue>1/2</issue><spage>217</spage><epage>222</epage><pages>217-222</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H, 31P‐NMR spectroscopy, FPLC and gel electrophoresis. An enexpected finding was the high level of 2′,3′‐cyclic nucleotides, detected by characteristic 31P‐NMR resonances in the region 20–21 ppm, originating from the yeast component. 31P‐NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1990.tb04152.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1990-03, Vol.68 (1/2), p.217-222 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15568053 |
| source | Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford University Press Journals Digital Archive Legacy |
| subjects | 31P‐NMR Biological and medical sciences culture media Cyclic nucleosides extracts Fundamental and applied biological sciences. Psychology General aspects LB broth Microbiology nuclear magnetic resonance spectroscopy nucleosides Yeast autolysis Yeast extracts yeasts |
| title | NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleosides in yeast extracts |
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