Purification of recombinant human tissue factor

Tissue factor (TF) is a 263 amino acid membrane-bound procoagulant protein that serves as a cofactor for the serine protease factor VII (fVII). Recombinant human TF (rTF) produced in both human kidney 293 cells and Escherichia coli has been immunoaffinity purified by using a TF-specific monoclonal a...

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Veröffentlicht in:	Biochemistry (Easton) 1989-10, Vol.28 (20), p.8072-8077
Hauptverfasser:	Paborsky, Lisa R, Tate, Keri M, Harris, Reed J, Yansura, Daniel G, Band, Louis, McCray, Glynis, Gorman, Cornelia M, O'Brien, Donogh P, Chang, Judy Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Cells, Cultured Cyanogen Bromide Cysteine - metabolism Cytoplasm - metabolism Escherichia coli - metabolism Humans Immunoassay Kidney - metabolism Molecular Sequence Data Plasmids Prothrombin Time Recombinant Proteins - isolation & purification Recombinant Proteins - pharmacology Thromboplastin - isolation & purification Thromboplastin - pharmacology Trypsin
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