Multiplex real‐time PCR assays for detection of four seedborne spinach pathogens

AIMS: To develop multiplex TaqMan real‐time PCR assays for detection of spinach seedborne pathogens that cause economically important diseases on spinach. METHODS AND RESULTS: Primers and probes were designed from conserved sequences of the internal transcribed spacer (for Peronospora farinosa f. sp...

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Veröffentlicht in:	Journal of applied microbiology 2014-08, Vol.117 (2), p.472-484
Hauptverfasser:	Feng, C, Mansouri, S, Bluhm, B.H, du Toit, L.J, Correll, J.C
Format:	Artikel
Sprache:	eng
Schlagworte:	Ascomycota - genetics Ascomycota - isolation & purification Biological and medical sciences Cladosporium Cladosporium - genetics Cladosporium - isolation & purification Cladosporium leaf spot conserved sequences Deoxyribonucleic acid DNA downy mildew Economic importance Food contamination & poisoning Fundamental and applied biological sciences. Psychology fungi intergenic DNA internal transcribed spacers Microbiology Multiplex Polymerase Chain Reaction - methods Oomycetes Pathogens Peronospora Peronospora - genetics Peronospora - isolation & purification Peronospora farinosa Polymerase chain reaction quantitative polymerase chain reaction Real-Time Polymerase Chain Reaction - methods real‐time PCR saprophytes seed assay,spinach seed industry Seeds - microbiology Spinach Spinacia oleracea Spinacia oleracea - microbiology Stemphylium Stemphylium botryosum Stemphylium leaf spot Vegetables Verticillium - genetics Verticillium - isolation & purification Verticillium dahliae Verticillium wilt
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