Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination
Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses....
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| Veröffentlicht in: | Gene therapy 2014-07, Vol.21 (7), p.703-708 |
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| creator | Peng, J Shi, S Yang, Z Ding, Q Hai, W Tang, H Yang, Y Bernstein, J R Peyda, P Xu, Y |
| description | Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses. To further improve the efficiency of EP-mediated DNA immunization, many parameters have been tried and optimized in recent years. In our early research, we found that the short noncoding DNA fragments (sf-DNA) can significantly enhance EP-mediated transgene expression of reporter genes. In this study, we tested the effect of sf-DNA on the immune potency of EP-mediated hepatitis B virus (HBV) DNA vaccination in a mouse model. The results show that the use of sf-DNA in EP-mediated HBV DNA vaccination leads to an enhanced expression of the HBV surface antigen, resulting in higher cellular and humoral responses. Furthermore, the immune responses in the sf-DNA-mediated 120 V cm
−1
EP immunization group were higher than that of the 200 V cm
−1
EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application. |
| doi_str_mv | 10.1038/gt.2014.44 |
| format | Article |
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−1
EP immunization group were higher than that of the 200 V cm
−1
EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application.</description><identifier>ISSN: 0969-7128</identifier><identifier>EISSN: 1476-5462</identifier><identifier>DOI: 10.1038/gt.2014.44</identifier><identifier>PMID: 24830435</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/250/251/1567 ; 631/61/24/590/1991 ; 692/699/255 ; Alkaline Phosphatase - genetics ; Alkaline Phosphatase - metabolism ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Clinical trials ; Deoxyribonucleic acid ; Disease Models, Animal ; DNA ; DNA vaccines ; DNA viruses ; Electroporation ; Electroporation - methods ; Female ; Gene Expression ; Gene Therapy ; Genetic vectors ; Genetic Vectors - administration & dosage ; Hepatitis ; Hepatitis B ; Hepatitis B - immunology ; Hepatitis B Surface Antigens - genetics ; Hepatitis B Surface Antigens - metabolism ; Hepatitis B Vaccines - immunology ; Hepatitis B virus ; Human Genetics ; Immune response (humoral) ; Immune system ; Immunization ; Methods ; Mice ; Mice, Inbred ICR ; Muscle, Skeletal - immunology ; Muscle, Skeletal - metabolism ; Nanotechnology ; original-article ; Plasmids - genetics ; Testing ; Vaccination ; Vaccination - methods ; Vaccines, DNA - immunology</subject><ispartof>Gene therapy, 2014-07, Vol.21 (7), p.703-708</ispartof><rights>Macmillan Publishers Limited 2014</rights><rights>COPYRIGHT 2014 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jul 2014</rights><rights>Macmillan Publishers Limited 2014.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c650t-dda7098e41161fd78348d4bcb506094700a18f7f279e9e4c2cf5d8fa07fc5083</citedby><cites>FETCH-LOGICAL-c650t-dda7098e41161fd78348d4bcb506094700a18f7f279e9e4c2cf5d8fa07fc5083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/gt.2014.44$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/gt.2014.44$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24830435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, J</creatorcontrib><creatorcontrib>Shi, S</creatorcontrib><creatorcontrib>Yang, Z</creatorcontrib><creatorcontrib>Ding, Q</creatorcontrib><creatorcontrib>Hai, W</creatorcontrib><creatorcontrib>Tang, H</creatorcontrib><creatorcontrib>Yang, Y</creatorcontrib><creatorcontrib>Bernstein, J R</creatorcontrib><creatorcontrib>Peyda, P</creatorcontrib><creatorcontrib>Xu, Y</creatorcontrib><title>Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination</title><title>Gene therapy</title><addtitle>Gene Ther</addtitle><addtitle>Gene Ther</addtitle><description>Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses. To further improve the efficiency of EP-mediated DNA immunization, many parameters have been tried and optimized in recent years. In our early research, we found that the short noncoding DNA fragments (sf-DNA) can significantly enhance EP-mediated transgene expression of reporter genes. In this study, we tested the effect of sf-DNA on the immune potency of EP-mediated hepatitis B virus (HBV) DNA vaccination in a mouse model. The results show that the use of sf-DNA in EP-mediated HBV DNA vaccination leads to an enhanced expression of the HBV surface antigen, resulting in higher cellular and humoral responses. Furthermore, the immune responses in the sf-DNA-mediated 120 V cm
−1
EP immunization group were higher than that of the 200 V cm
−1
EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application.</description><subject>631/250/251/1567</subject><subject>631/61/24/590/1991</subject><subject>692/699/255</subject><subject>Alkaline Phosphatase - genetics</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Clinical trials</subject><subject>Deoxyribonucleic acid</subject><subject>Disease Models, Animal</subject><subject>DNA</subject><subject>DNA vaccines</subject><subject>DNA viruses</subject><subject>Electroporation</subject><subject>Electroporation - methods</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Therapy</subject><subject>Genetic vectors</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Hepatitis</subject><subject>Hepatitis B</subject><subject>Hepatitis B - immunology</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B Surface Antigens - metabolism</subject><subject>Hepatitis B Vaccines - immunology</subject><subject>Hepatitis B virus</subject><subject>Human Genetics</subject><subject>Immune response (humoral)</subject><subject>Immune system</subject><subject>Immunization</subject><subject>Methods</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Muscle, Skeletal - immunology</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Nanotechnology</subject><subject>original-article</subject><subject>Plasmids - genetics</subject><subject>Testing</subject><subject>Vaccination</subject><subject>Vaccination - methods</subject><subject>Vaccines, DNA - immunology</subject><issn>0969-7128</issn><issn>1476-5462</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqN0ltrFDEUAOBBFLtWX_wBMiCIF2bNdZJ5XOulhaJgi68hmzmZnTKTrEmm2H9vplu1qyKSh4ScLye3UxSPMVpiROXrLi0JwmzJ2J1igZmoK85qcrdYoKZuKoGJPCgexHiBEGJCkvvFAWGSIkb5oticbXxIpfPO-LZ3Xfn246q0QXcjuBTLftwGfwll2kAej5ODcusTOHNVelvCACYFv_VBp967aoS21wna8vjNl-tEl9qY3l0HHxb3rB4iPLrpD4vz9-_Oj46r008fTo5Wp5WpOUpV22qBGgkM4xrbVkjKZMvWZs1RjRomENJYWmGJaKABZoixvJVWI2ENR5IeFs93afO5v04Qkxr7aGAYtAM_RYU5Zw1HvMH_QRklklOCMn36G73wU3D5HorUjNW1aBr-L5VzESpRxr9UpwdQvbM-BW3mrdWKCsEpwnRWy7-o3FoYe-Md2D7P7y14sbcgmwTfUqenGNXJ2ed9--yW3YAe0ib6YZr_Ke7Dlztogo8xgFXb0I86XCmM1Fx7qktqrj3FZvzk5vrTOlfCT_qj2DJ4tQMxh1wH4db7_JnuOyC03Q8</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Peng, J</creator><creator>Shi, S</creator><creator>Yang, Z</creator><creator>Ding, Q</creator><creator>Hai, W</creator><creator>Tang, H</creator><creator>Yang, Y</creator><creator>Bernstein, J R</creator><creator>Peyda, P</creator><creator>Xu, Y</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope><scope>7T5</scope></search><sort><creationdate>20140701</creationdate><title>Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination</title><author>Peng, J ; Shi, S ; Yang, Z ; Ding, Q ; Hai, W ; Tang, H ; Yang, Y ; Bernstein, J R ; Peyda, P ; Xu, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c650t-dda7098e41161fd78348d4bcb506094700a18f7f279e9e4c2cf5d8fa07fc5083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>631/250/251/1567</topic><topic>631/61/24/590/1991</topic><topic>692/699/255</topic><topic>Alkaline Phosphatase - 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Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><jtitle>Gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, J</au><au>Shi, S</au><au>Yang, Z</au><au>Ding, Q</au><au>Hai, W</au><au>Tang, H</au><au>Yang, Y</au><au>Bernstein, J R</au><au>Peyda, P</au><au>Xu, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination</atitle><jtitle>Gene therapy</jtitle><stitle>Gene Ther</stitle><addtitle>Gene Ther</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>21</volume><issue>7</issue><spage>703</spage><epage>708</epage><pages>703-708</pages><issn>0969-7128</issn><eissn>1476-5462</eissn><abstract>Electroporation (EP)-mediated DNA immunization can elicit effective immune responses in a variety of animals, and is widely used in research studies and clinical trials. However, high-pulse voltage, high DNA dose and multiple immunizations are still required to achieve considerable immune responses. To further improve the efficiency of EP-mediated DNA immunization, many parameters have been tried and optimized in recent years. In our early research, we found that the short noncoding DNA fragments (sf-DNA) can significantly enhance EP-mediated transgene expression of reporter genes. In this study, we tested the effect of sf-DNA on the immune potency of EP-mediated hepatitis B virus (HBV) DNA vaccination in a mouse model. The results show that the use of sf-DNA in EP-mediated HBV DNA vaccination leads to an enhanced expression of the HBV surface antigen, resulting in higher cellular and humoral responses. Furthermore, the immune responses in the sf-DNA-mediated 120 V cm
−1
EP immunization group were higher than that of the 200 V cm
−1
EP without sf-DNA groups. These data suggest that the sf-DNA can be used as an effective helper molecule to improve the immune response of EP-mediated HBV DNA vaccination, which may make the EP-mediated DNA vaccination more effective and suitable for animal and clinical application.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>24830435</pmid><doi>10.1038/gt.2014.44</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Gene therapy, 2014-07, Vol.21 (7), p.703-708 |
| issn | 0969-7128 1476-5462 |
| language | eng |
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| subjects | 631/250/251/1567 631/61/24/590/1991 692/699/255 Alkaline Phosphatase - genetics Alkaline Phosphatase - metabolism Animals Biomedical and Life Sciences Biomedicine Cell Biology Clinical trials Deoxyribonucleic acid Disease Models, Animal DNA DNA vaccines DNA viruses Electroporation Electroporation - methods Female Gene Expression Gene Therapy Genetic vectors Genetic Vectors - administration & dosage Hepatitis Hepatitis B Hepatitis B - immunology Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - metabolism Hepatitis B Vaccines - immunology Hepatitis B virus Human Genetics Immune response (humoral) Immune system Immunization Methods Mice Mice, Inbred ICR Muscle, Skeletal - immunology Muscle, Skeletal - metabolism Nanotechnology original-article Plasmids - genetics Testing Vaccination Vaccination - methods Vaccines, DNA - immunology |
| title | Short noncoding DNA fragments improve the immune potency of electroporation-mediated HBV DNA vaccination |
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