Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay
Dengue virus infects millions of people worldwide and there is no vaccine or anti-dengue therapeutic available. Screening large numbers of medicinal plants for anti-dengue activities is an alternative strategy in order to find the potent therapeutic compounds. Therefore, this study was designed to i...
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| Veröffentlicht in: | Tropical biomedicine 2014-06, Vol.31 (2), p.286-296 |
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| creator | Rothan, H A Zulqarnain, M Ammar, Y A Tan, E C Rahman, N A Yusof, R |
| description | Dengue virus infects millions of people worldwide and there is no vaccine or anti-dengue therapeutic available. Screening large numbers of medicinal plants for anti-dengue activities is an alternative strategy in order to find the potent therapeutic compounds. Therefore, this study was designed to identify anti-dengue activities in nineteen medicinal plant extracts that are used in traditional medicine. Local medicinal plants Vernonia cinerea, Hemigraphis reptans, Hedyotis auricularia, Laurentia longiflora, Tridax procumbers and Senna angustifolia were used in this study. The highest inhibitory activates against dengue NS2B-NS3pro was observed in ethanolic extract of S. angustifolia leaves, methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems. These findings were further verified by in vitro viral inhibition assay. Methanolic extract of V. cinerea leaves, ethanol extract of T. procumbens stems and at less extent ethanolic extract of S. angustifolia leaves were able to maintain the normal morphology of DENV2-infected Vero cells without causing much cytopathic effects (CPE). The percentage of viral inhibition of V. cinerea and T. procumbens extracts were significantly higher than S. angustifolia extract as measured by plaque formation assay and RT-qPCR. In conclusion, The outcome of this study showed that the methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems possessed high inhibitory activates against dengue virus that worth more investigation. |
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Screening large numbers of medicinal plants for anti-dengue activities is an alternative strategy in order to find the potent therapeutic compounds. Therefore, this study was designed to identify anti-dengue activities in nineteen medicinal plant extracts that are used in traditional medicine. Local medicinal plants Vernonia cinerea, Hemigraphis reptans, Hedyotis auricularia, Laurentia longiflora, Tridax procumbers and Senna angustifolia were used in this study. The highest inhibitory activates against dengue NS2B-NS3pro was observed in ethanolic extract of S. angustifolia leaves, methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems. These findings were further verified by in vitro viral inhibition assay. Methanolic extract of V. cinerea leaves, ethanol extract of T. procumbens stems and at less extent ethanolic extract of S. angustifolia leaves were able to maintain the normal morphology of DENV2-infected Vero cells without causing much cytopathic effects (CPE). The percentage of viral inhibition of V. cinerea and T. procumbens extracts were significantly higher than S. angustifolia extract as measured by plaque formation assay and RT-qPCR. In conclusion, The outcome of this study showed that the methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems possessed high inhibitory activates against dengue virus that worth more investigation.</description><identifier>ISSN: 0127-5720</identifier><identifier>PMID: 25134897</identifier><language>eng</language><publisher>Malaysia</publisher><subject>Animals ; Antiviral Agents - isolation & purification ; Antiviral Agents - pharmacology ; Antiviral Agents - toxicity ; Cell Survival - drug effects ; Cercopithecus aethiops ; Drug Evaluation, Preclinical - methods ; Microbial Sensitivity Tests ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Extracts - toxicity ; Plants, Medicinal - chemistry ; Protease Inhibitors - isolation & purification ; Protease Inhibitors - pharmacology ; Protease Inhibitors - toxicity ; Real-Time Polymerase Chain Reaction ; Serine Endopeptidases - metabolism ; Vero Cells ; Viral Nonstructural Proteins - metabolism ; Viral Plaque Assay</subject><ispartof>Tropical biomedicine, 2014-06, Vol.31 (2), p.286-296</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25134897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rothan, H A</creatorcontrib><creatorcontrib>Zulqarnain, M</creatorcontrib><creatorcontrib>Ammar, Y A</creatorcontrib><creatorcontrib>Tan, E C</creatorcontrib><creatorcontrib>Rahman, N A</creatorcontrib><creatorcontrib>Yusof, R</creatorcontrib><title>Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay</title><title>Tropical biomedicine</title><addtitle>Trop Biomed</addtitle><description>Dengue virus infects millions of people worldwide and there is no vaccine or anti-dengue therapeutic available. Screening large numbers of medicinal plants for anti-dengue activities is an alternative strategy in order to find the potent therapeutic compounds. Therefore, this study was designed to identify anti-dengue activities in nineteen medicinal plant extracts that are used in traditional medicine. Local medicinal plants Vernonia cinerea, Hemigraphis reptans, Hedyotis auricularia, Laurentia longiflora, Tridax procumbers and Senna angustifolia were used in this study. The highest inhibitory activates against dengue NS2B-NS3pro was observed in ethanolic extract of S. angustifolia leaves, methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems. These findings were further verified by in vitro viral inhibition assay. Methanolic extract of V. cinerea leaves, ethanol extract of T. procumbens stems and at less extent ethanolic extract of S. angustifolia leaves were able to maintain the normal morphology of DENV2-infected Vero cells without causing much cytopathic effects (CPE). The percentage of viral inhibition of V. cinerea and T. procumbens extracts were significantly higher than S. angustifolia extract as measured by plaque formation assay and RT-qPCR. In conclusion, The outcome of this study showed that the methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems possessed high inhibitory activates against dengue virus that worth more investigation.</description><subject>Animals</subject><subject>Antiviral Agents - isolation & purification</subject><subject>Antiviral Agents - pharmacology</subject><subject>Antiviral Agents - toxicity</subject><subject>Cell Survival - drug effects</subject><subject>Cercopithecus aethiops</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Microbial Sensitivity Tests</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - toxicity</subject><subject>Plants, Medicinal - chemistry</subject><subject>Protease Inhibitors - isolation & purification</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Protease Inhibitors - toxicity</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Serine Endopeptidases - metabolism</subject><subject>Vero Cells</subject><subject>Viral Nonstructural Proteins - metabolism</subject><subject>Viral Plaque Assay</subject><issn>0127-5720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE1PwzAMhnMAsTH4CyhHLpWar7U9wsSXNI1D4Vy5iTsFdVmpU7T9ezIxDpat149fy75g81zIIjOFzGfsmugrz40uS33FZtIIpcuqmLNDbUfE4MOW7zsOIfofP0LPwZ6q6JG4D3yHzlsfkj70iSGOhzgmhDhswQeK3GHYTsjT8ER8opPfWdrU8jHb1IoP4z4iEHIgguMNu-ygJ7w95wX7fH76WL1m6_eXt9XDOhukEDHTrnKtgmXZWa0ktFil6Iwp29Z0aJ1VArVaFkKXaNulUdIJqUBi6sjKObVg93--af33hBSbnSeLfboD9xM1whhdaVFUeULvzujUpoubYfQ7GI_N_7fUL6ISaA0</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Rothan, H A</creator><creator>Zulqarnain, M</creator><creator>Ammar, Y A</creator><creator>Tan, E C</creator><creator>Rahman, N A</creator><creator>Yusof, R</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay</title><author>Rothan, H A ; Zulqarnain, M ; Ammar, Y A ; Tan, E C ; Rahman, N A ; Yusof, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-4d9db3a68fc432abe9abef558bb5fecdc31e4367148ecb6532d123a2edc329dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Antiviral Agents - isolation & purification</topic><topic>Antiviral Agents - pharmacology</topic><topic>Antiviral Agents - toxicity</topic><topic>Cell Survival - drug effects</topic><topic>Cercopithecus aethiops</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Microbial Sensitivity Tests</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - toxicity</topic><topic>Plants, Medicinal - chemistry</topic><topic>Protease Inhibitors - isolation & purification</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Protease Inhibitors - toxicity</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Serine Endopeptidases - metabolism</topic><topic>Vero Cells</topic><topic>Viral Nonstructural Proteins - metabolism</topic><topic>Viral Plaque Assay</topic><toplevel>online_resources</toplevel><creatorcontrib>Rothan, H A</creatorcontrib><creatorcontrib>Zulqarnain, M</creatorcontrib><creatorcontrib>Ammar, Y A</creatorcontrib><creatorcontrib>Tan, E C</creatorcontrib><creatorcontrib>Rahman, N A</creatorcontrib><creatorcontrib>Yusof, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Tropical biomedicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rothan, H A</au><au>Zulqarnain, M</au><au>Ammar, Y A</au><au>Tan, E C</au><au>Rahman, N A</au><au>Yusof, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay</atitle><jtitle>Tropical biomedicine</jtitle><addtitle>Trop Biomed</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>31</volume><issue>2</issue><spage>286</spage><epage>296</epage><pages>286-296</pages><issn>0127-5720</issn><abstract>Dengue virus infects millions of people worldwide and there is no vaccine or anti-dengue therapeutic available. Screening large numbers of medicinal plants for anti-dengue activities is an alternative strategy in order to find the potent therapeutic compounds. Therefore, this study was designed to identify anti-dengue activities in nineteen medicinal plant extracts that are used in traditional medicine. Local medicinal plants Vernonia cinerea, Hemigraphis reptans, Hedyotis auricularia, Laurentia longiflora, Tridax procumbers and Senna angustifolia were used in this study. The highest inhibitory activates against dengue NS2B-NS3pro was observed in ethanolic extract of S. angustifolia leaves, methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems. These findings were further verified by in vitro viral inhibition assay. Methanolic extract of V. cinerea leaves, ethanol extract of T. procumbens stems and at less extent ethanolic extract of S. angustifolia leaves were able to maintain the normal morphology of DENV2-infected Vero cells without causing much cytopathic effects (CPE). The percentage of viral inhibition of V. cinerea and T. procumbens extracts were significantly higher than S. angustifolia extract as measured by plaque formation assay and RT-qPCR. In conclusion, The outcome of this study showed that the methanolic extract of V. cinerea leaves and ethanol extract of T. procumbens stems possessed high inhibitory activates against dengue virus that worth more investigation.</abstract><cop>Malaysia</cop><pmid>25134897</pmid><tpages>11</tpages></addata></record> |
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| ispartof | Tropical biomedicine, 2014-06, Vol.31 (2), p.286-296 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antiviral Agents - isolation & purification Antiviral Agents - pharmacology Antiviral Agents - toxicity Cell Survival - drug effects Cercopithecus aethiops Drug Evaluation, Preclinical - methods Microbial Sensitivity Tests Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Extracts - toxicity Plants, Medicinal - chemistry Protease Inhibitors - isolation & purification Protease Inhibitors - pharmacology Protease Inhibitors - toxicity Real-Time Polymerase Chain Reaction Serine Endopeptidases - metabolism Vero Cells Viral Nonstructural Proteins - metabolism Viral Plaque Assay |
| title | Screening of antiviral activities in medicinal plants extracts against dengue virus using dengue NS2B-NS3 protease assay |
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