MIRA Analysis of RARβ2 Gene Methylation in DNA Circulating in the Blood in Lung Cancer
Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment effi ciency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisul...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2014-08, Vol.157 (4), p.516-519 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Kapitskaya, K. Yu Azhikina, T. L. Ponomaryova, A. A. Cherdyntseva, N. V. Vlasov, V. V. Laktionov, P. P. Rykova, E. Yu |
| description | Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment effi ciency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfi te conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for
RARβ2
tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors. |
| doi_str_mv | 10.1007/s10517-014-2604-z |
| format | Article |
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RARβ2
tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-014-2604-z</identifier><identifier>PMID: 25110096</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Aged ; Biological Assay ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - genetics ; Biomedical and Life Sciences ; Biomedicine ; Cancer ; Carcinoma, Non-Small-Cell Lung - blood ; Carcinoma, Non-Small-Cell Lung - diagnosis ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Care and treatment ; Case-Control Studies ; Cell Biology ; CpG Islands ; DNA ; DNA Methylation ; DNA, Neoplasm - blood ; DNA, Neoplasm - genetics ; Epigenesis, Genetic ; Epigenetic inheritance ; Female ; Genes ; Genetic aspects ; Humans ; Internal Medicine ; Laboratory Medicine ; Lung cancer ; Lung Neoplasms - blood ; Lung Neoplasms - diagnosis ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Male ; Methylation ; Middle Aged ; Pathology ; Protein binding ; Receptors, Retinoic Acid - blood ; Receptors, Retinoic Acid - genetics ; Sulfites ; Sulfites - chemistry</subject><ispartof>Bulletin of experimental biology and medicine, 2014-08, Vol.157 (4), p.516-519</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>COPYRIGHT 2014 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427z-703ad59d5d76967c2736ec1844e50f3f6917ac9a9b0c37f4a762e3f005b01ee63</citedby><cites>FETCH-LOGICAL-c427z-703ad59d5d76967c2736ec1844e50f3f6917ac9a9b0c37f4a762e3f005b01ee63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-014-2604-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-014-2604-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25110096$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapitskaya, K. Yu</creatorcontrib><creatorcontrib>Azhikina, T. L.</creatorcontrib><creatorcontrib>Ponomaryova, A. A.</creatorcontrib><creatorcontrib>Cherdyntseva, N. V.</creatorcontrib><creatorcontrib>Vlasov, V. V.</creatorcontrib><creatorcontrib>Laktionov, P. P.</creatorcontrib><creatorcontrib>Rykova, E. Yu</creatorcontrib><title>MIRA Analysis of RARβ2 Gene Methylation in DNA Circulating in the Blood in Lung Cancer</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment effi ciency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfi te conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for
RARβ2
tumor suppression gene in circulating DNA in lung cancer and a trend was detected to higher methylation of this gene in the patients in comparison with healthy donors.</description><subject>Aged</subject><subject>Biological Assay</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cancer</subject><subject>Carcinoma, Non-Small-Cell Lung - blood</subject><subject>Carcinoma, Non-Small-Cell Lung - diagnosis</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Care and treatment</subject><subject>Case-Control Studies</subject><subject>Cell Biology</subject><subject>CpG Islands</subject><subject>DNA</subject><subject>DNA Methylation</subject><subject>DNA, Neoplasm - blood</subject><subject>DNA, Neoplasm - genetics</subject><subject>Epigenesis, Genetic</subject><subject>Epigenetic inheritance</subject><subject>Female</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - blood</subject><subject>Lung Neoplasms - diagnosis</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Methylation</subject><subject>Middle Aged</subject><subject>Pathology</subject><subject>Protein binding</subject><subject>Receptors, Retinoic Acid - blood</subject><subject>Receptors, Retinoic Acid - genetics</subject><subject>Sulfites</subject><subject>Sulfites - chemistry</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd9qFDEYxYNY7Fp9AG8kIIg3U5NM_sxcjqvWwlZhUbwM2cw3u1OySU1mLnYfywfxmcwwbWlBJBfhO_mdA_kOQq8oOaeEqPeJEkFVQSgvmCS8OD5BCypUWVSM0adoQTJU8KqqTtHzlK6nkUj6DJ0yQXNALRfo59XlusGNN-6Q-oRDh9fN-s9vhi_AA76CYXdwZuiDx73HH782eNlHO06S307SsAP8wYXQTsNqzOLSeAvxBTrpjEvw8vY-Qz8-f_q-_FKsvl1cLptVYTlTx0KR0rSibkWrZC2VZaqUYGnFOQjSlZ2sqTK2NvWG2FJ13CjJoOwIERtCAWR5ht7NuTcx_BohDXrfJwvOGQ9hTJoKwbmsWc0z-mZGt8aB7n0XhmjshOumrGTejpQsU-f_oPJpYd_b4KHrs_7I8PaBYQfGDbsU3DgtLT0G6QzaGFKK0Omb2O9NPGhK9NSnnvvUuU899amP2fP69nvjZg_tveOuwAywGUj5yW8h6uswxlxn-k_qX71JpsA</recordid><startdate>20140801</startdate><enddate>20140801</enddate><creator>Kapitskaya, K. Yu</creator><creator>Azhikina, T. L.</creator><creator>Ponomaryova, A. A.</creator><creator>Cherdyntseva, N. V.</creator><creator>Vlasov, V. V.</creator><creator>Laktionov, P. P.</creator><creator>Rykova, E. Yu</creator><general>Springer US</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140801</creationdate><title>MIRA Analysis of RARβ2 Gene Methylation in DNA Circulating in the Blood in Lung Cancer</title><author>Kapitskaya, K. Yu ; Azhikina, T. L. ; Ponomaryova, A. A. ; Cherdyntseva, N. V. ; Vlasov, V. V. ; Laktionov, P. P. ; Rykova, E. 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Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MIRA Analysis of RARβ2 Gene Methylation in DNA Circulating in the Blood in Lung Cancer</atitle><jtitle>Bulletin of experimental biology and medicine</jtitle><stitle>Bull Exp Biol Med</stitle><addtitle>Bull Exp Biol Med</addtitle><date>2014-08-01</date><risdate>2014</risdate><volume>157</volume><issue>4</issue><spage>516</spage><epage>519</epage><pages>516-519</pages><issn>0007-4888</issn><eissn>1573-8221</eissn><abstract>Analysis of DNA epigenetic mutations in the blood circulating DNA is a prospective trend for creation of noninvasive methods for the diagnosis and treatment effi ciency monitoring in cancer. The methylation status of target genes in circulating DNA was evaluated by methods based on preliminary bisulfi te conversion of DNA. We used a different approach based on selection of hypermethylated sequences of circulating DNA by means of DNA-methyl-binding protein (methylated CpG island recovery assay, MIRA). Methylation was evaluated for
RARβ2
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| subjects | Aged Biological Assay Biomarkers, Tumor - blood Biomarkers, Tumor - genetics Biomedical and Life Sciences Biomedicine Cancer Carcinoma, Non-Small-Cell Lung - blood Carcinoma, Non-Small-Cell Lung - diagnosis Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - pathology Care and treatment Case-Control Studies Cell Biology CpG Islands DNA DNA Methylation DNA, Neoplasm - blood DNA, Neoplasm - genetics Epigenesis, Genetic Epigenetic inheritance Female Genes Genetic aspects Humans Internal Medicine Laboratory Medicine Lung cancer Lung Neoplasms - blood Lung Neoplasms - diagnosis Lung Neoplasms - genetics Lung Neoplasms - pathology Male Methylation Middle Aged Pathology Protein binding Receptors, Retinoic Acid - blood Receptors, Retinoic Acid - genetics Sulfites Sulfites - chemistry |
| title | MIRA Analysis of RARβ2 Gene Methylation in DNA Circulating in the Blood in Lung Cancer |
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